##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062718_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4504610 Sequences flagged as poor quality 0 Sequence length 38 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.318764776528933 32.0 32.0 32.0 32.0 32.0 2 30.980601206319747 32.0 32.0 32.0 32.0 32.0 3 31.01055918270394 32.0 32.0 32.0 32.0 32.0 4 31.089548040784884 32.0 32.0 32.0 32.0 32.0 5 31.00783508450232 32.0 32.0 32.0 32.0 32.0 6 34.66507844186289 36.0 36.0 36.0 32.0 36.0 7 34.62463098914223 36.0 36.0 36.0 32.0 36.0 8 34.6140480529946 36.0 36.0 36.0 32.0 36.0 9 34.74235505404464 36.0 36.0 36.0 32.0 36.0 10 34.489149560117305 36.0 36.0 36.0 32.0 36.0 11 34.708128783623884 36.0 36.0 36.0 32.0 36.0 12 34.556068782869104 36.0 36.0 36.0 32.0 36.0 13 34.62128241956573 36.0 36.0 36.0 32.0 36.0 14 34.53524322860358 36.0 36.0 36.0 32.0 36.0 15 34.48767862256666 36.0 36.0 36.0 32.0 36.0 16 34.503118138973186 36.0 36.0 36.0 32.0 36.0 17 34.45164154055512 36.0 36.0 36.0 32.0 36.0 18 34.46407502536291 36.0 36.0 36.0 32.0 36.0 19 34.449752364799615 36.0 36.0 36.0 32.0 36.0 20 34.43856271686117 36.0 36.0 36.0 32.0 36.0 21 34.429862074630215 36.0 36.0 36.0 32.0 36.0 22 34.403631390952825 36.0 36.0 36.0 32.0 36.0 23 34.35182735020346 36.0 36.0 36.0 32.0 36.0 24 34.33853274756305 36.0 36.0 36.0 32.0 36.0 25 34.318775432279374 36.0 36.0 36.0 32.0 36.0 26 34.276154872452885 36.0 36.0 36.0 32.0 36.0 27 34.278271148889694 36.0 36.0 36.0 32.0 36.0 28 34.26158979356703 36.0 36.0 36.0 32.0 36.0 29 34.232931596742006 36.0 36.0 36.0 32.0 36.0 30 34.20909246305452 36.0 36.0 36.0 32.0 36.0 31 34.22637586827717 36.0 36.0 36.0 32.0 36.0 32 34.18717824628547 36.0 36.0 36.0 32.0 36.0 33 34.16129276452345 36.0 36.0 36.0 32.0 36.0 34 34.163765342615676 36.0 36.0 36.0 32.0 36.0 35 34.14410215312758 36.0 36.0 36.0 32.0 36.0 36 34.1052612767809 36.0 36.0 36.0 32.0 36.0 37 34.09783865861861 36.0 36.0 36.0 32.0 36.0 38 33.563452773936035 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 12.0 8 4.0 9 14.0 10 43.0 11 47.0 12 25.0 13 19.0 14 272.0 15 625.0 16 1038.0 17 1296.0 18 1502.0 19 1983.0 20 2644.0 21 4046.0 22 6461.0 23 10379.0 24 16004.0 25 25223.0 26 37839.0 27 54909.0 28 78109.0 29 105964.0 30 143756.0 31 194949.0 32 268413.0 33 410254.0 34 950872.0 35 2187906.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.91001699906114 17.067364114199552 11.593301907593387 26.429316979145923 2 16.779843939524177 19.18389757606732 37.52661181827934 26.509646666129154 3 18.816855991140486 23.06324660019662 28.608680477539938 29.511216931122952 4 12.834728316780161 15.632036060148199 35.75567313741848 35.77756248565315 5 14.796367001813035 36.14186301599765 34.10965104774032 14.952118934448993 6 34.62168416435125 35.28840322195376 16.812317908198647 13.277594705496343 7 30.629901647379747 30.0298494341794 20.830691594029936 18.509557324410913 8 28.14616728884658 33.30956499500954 19.12008901139104 19.42417870475284 9 27.493082063981333 14.241435689556997 18.224923661283647 40.04055858517802 10 15.814345440785724 26.847336078538714 31.961929422514544 25.376389058161013 11 37.39458897860145 20.878519528206176 22.42248324457044 19.304408248621936 12 24.648083373071586 23.314631934990885 29.105893782095855 22.931390909841678 13 29.830293220720943 19.51402603717408 24.81639984502475 25.839280897080226 14 23.653659916335382 19.259846533459854 25.076451309385472 32.01004224081929 15 25.35456114932192 27.199538139568446 21.893205877618087 25.55269483349154 16 25.838796315270766 25.405546898017207 23.75973640034431 24.995920386367718 17 24.354038215926916 25.66774400977691 24.997757385528963 24.980460388767213 18 24.82612355883693 25.129985516216774 25.73437931527493 24.309511609671368 19 25.729085100020182 24.835993455945186 25.01431428735718 24.420607156677452 20 25.92567431662544 24.583545906190878 24.59455751322392 24.896222263959768 21 26.73112513032003 24.26687766523337 24.252202785962556 24.749794418484043 22 26.034161950566254 24.343647811811554 24.69442755299383 24.927762684628366 23 25.115987040444992 24.351805783400128 24.990858465061354 25.541348711093526 24 25.27172766708748 24.68113601414578 24.84965445947764 25.197481859289095 25 25.304289460601844 24.431103768262417 24.917509519071782 25.347097252063953 26 24.880582290808352 25.190325616838848 25.28384811785967 24.645243974493138 27 25.41179453433116 24.703298352555443 24.680608358265683 25.20429875484771 28 25.031736101274905 24.744079812909078 24.973769707130955 25.25041437868506 29 24.838364545148362 24.803152315981134 25.061715759159895 25.296767379710605 30 24.908357901466985 25.072860796362733 25.102364756864276 24.916416545306006 31 25.50412036770832 24.650564090427636 24.59142101235455 25.253894529509484 32 25.08418644633736 24.92957821524734 24.447637451246457 25.53859788716885 33 24.879634616742322 24.73710104475776 24.8649372297246 25.518327108775317 34 25.53095971726553 24.5392443273933 25.081493974041823 24.848301981299347 35 25.51532961134492 24.677937726819078 25.059077037166965 24.747655624669036 36 24.744101035407777 24.905404751321402 24.961466951277618 25.389027261993203 37 25.4802749204676 24.7780541408691 24.649061674162365 25.092609264500943 38 24.98854431042965 24.806563133282065 24.874187223265174 25.33070533302311 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 38.0 1 99.0 2 160.0 3 160.0 4 465.5 5 771.0 6 771.0 7 924.0 8 1077.0 9 1109.0 10 1141.0 11 1141.0 12 1709.0 13 2277.0 14 3463.0 15 4649.0 16 4649.0 17 7410.5 18 10172.0 19 10172.0 20 14075.5 21 17979.0 22 22346.0 23 26713.0 24 26713.0 25 35542.0 26 44371.0 27 44371.0 28 58131.5 29 71892.0 30 89108.5 31 106325.0 32 106325.0 33 132426.0 34 158527.0 35 158527.0 36 178205.0 37 197883.0 38 222201.0 39 246519.0 40 246519.0 41 265823.0 42 285127.0 43 317933.5 44 350740.0 45 350740.0 46 364045.0 47 377350.0 48 377350.0 49 392122.5 50 406895.0 51 414486.5 52 422078.0 53 422078.0 54 414043.5 55 406009.0 56 406009.0 57 387041.0 58 368073.0 59 334799.0 60 301525.0 61 301525.0 62 275030.5 63 248536.0 64 207957.5 65 167379.0 66 167379.0 67 139648.5 68 111918.0 69 111918.0 70 90493.0 71 69068.0 72 54304.5 73 39541.0 74 39541.0 75 30830.5 76 22120.0 77 22120.0 78 18854.0 79 15588.0 80 12112.0 81 8636.0 82 8636.0 83 7593.5 84 6551.0 85 6551.0 86 4481.5 87 2412.0 88 1887.5 89 1363.0 90 1363.0 91 1048.0 92 733.0 93 632.5 94 532.0 95 532.0 96 473.5 97 415.0 98 415.0 99 971.0 100 1527.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12294072072832055 2 0.052856962089947855 3 0.011676926526380752 4 0.0030191292919919815 5 2.441942809699397E-4 6 3.107927212344687E-4 7 4.439896017635267E-5 8 3.9959064158717403E-4 9 3.32992201322645E-4 10 6.437849225571137E-4 11 0.0022199480088176335 12 0.002974730331815629 13 0.014762654258637263 14 0.009678973318444882 15 0.024086435895671325 16 0.011255136404705402 17 0.020823112322709402 18 0.0058162637831022 19 0.008191608152537068 20 0.006038258583983963 21 0.006970636747687369 22 0.007969613351655304 23 0.009834369679062116 24 0.01447406101749097 25 0.016805006426749487 26 0.019557741957683353 27 0.009368180597210413 28 0.006127056504336668 29 0.010944343683470933 30 0.0028859324114629235 31 0.0061936549446011974 32 0.00714823258839278 33 0.009057387875975945 34 0.013230890132553096 35 0.01653861266569137 36 0.015184444380312613 37 0.010455955121531054 38 0.006615445066276548 >>END_MODULE >>Sequence Length Distribution pass #Length Count 38 4504610.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.252477371712054 #Duplication Level Percentage of deduplicated Percentage of total 1 58.75486552269796 18.949899707488008 2 14.601422463912018 9.418640952242606 3 7.793318979578094 7.540615321181296 4 4.748347060725834 6.125838245163815 5 3.134357764854983 5.0545401442917655 6 2.249108589826619 4.352359433994374 7 1.6587811497843108 3.7449861048628694 8 1.264854027032844 3.2635740708316527 9 0.9262797190918652 2.688733411189756 >10 4.622848825486972 24.621955153014458 >50 0.14696464223651587 3.2326760792172045 >100 0.08212037087043171 5.339336544280876 >500 0.01092346998922903 2.466987757659338 >1k 0.005807413912520053 3.199857074582082 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 4989 0.11075320615991173 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.879792035270534E-5 2 0.0 0.0 0.0 0.0 8.879792035270534E-5 3 0.0 0.0 0.0 0.0 8.879792035270534E-5 4 0.0 0.0 0.0 0.0 8.879792035270534E-5 5 0.0 0.0 0.0 0.0 1.1099740044088167E-4 6 0.0 0.0 0.0 0.0 2.2199480088176335E-4 7 0.0 0.0 0.0 0.0 2.2199480088176335E-4 8 0.0 0.0 0.0 0.0 2.2199480088176335E-4 9 0.0 0.0 0.0 4.439896017635267E-5 2.2199480088176335E-4 10 0.0 0.0 0.0 4.439896017635267E-5 2.2199480088176335E-4 11 0.0 0.0 0.0 4.439896017635267E-5 2.441942809699397E-4 12 0.0 0.0 0.0 4.439896017635267E-5 6.6598440264529E-4 13 0.0 0.0 0.0 4.439896017635267E-5 7.769818030861717E-4 14 0.0 0.0 0.0 4.439896017635267E-5 7.769818030861717E-4 15 0.0 0.0 0.0 4.439896017635267E-5 0.0010211760840561114 16 0.0 0.0 0.0 4.439896017635267E-5 0.0010877745243206404 17 0.0 0.0 0.0 4.439896017635267E-5 0.001132173484496993 18 0.0 0.0 0.0 8.879792035270534E-5 0.0012209714048496984 19 0.0 0.0 0.0 8.879792035270534E-5 0.0012431708849378748 20 0.0 0.0 0.0 1.1099740044088167E-4 0.0014207667256432854 21 0.0 0.0 0.0 1.33196880529058E-4 0.0015095646459959908 22 0.0 0.0 0.0 1.7759584070541068E-4 0.0015761630862605198 23 2.2199480088176335E-5 0.0 0.0 2.441942809699397E-4 0.0015761630862605198 24 2.2199480088176335E-5 0.0 0.0 4.2179012167535036E-4 0.0015761630862605198 25 2.2199480088176335E-5 0.0 0.0 5.771864822925847E-4 0.0015761630862605198 26 2.2199480088176335E-5 0.0 0.0 7.547823229979954E-4 0.0015761630862605198 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4430 0.0 21.623314 1 GGTATCA 1445 0.0 20.06483 1 ACCGTCG 800 0.0 15.598722 8 TACCGTC 865 0.0 14.611359 7 ACGATTA 165 4.8567017E-10 14.544587 32 TATCAAC 6600 0.0 14.43043 2 ATACCGT 945 0.0 14.218848 6 CGTCGTA 895 0.0 14.123003 10 TCAACGC 6620 0.0 14.111924 4 AACCGCG 320 0.0 13.998698 7 ATCAACG 6700 0.0 13.944973 3 CAACGCA 6720 0.0 13.877813 5 CCGTCGT 940 0.0 13.617116 9 TAGGACG 985 0.0 13.479483 4 AACGCAG 6895 0.0 13.479034 6 TCTAGCG 630 0.0 13.459065 28 GTATTAG 830 0.0 13.316674 1 GTAAACG 545 0.0 13.209782 27 CTAGCGG 630 0.0 13.205708 29 GTCCTAT 715 0.0 12.770079 1 >>END_MODULE