FastQCFastQC Report
Thu 2 Feb 2017
SRR4062718_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062718_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4504610
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46720.10371597097195984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39900.020.2116761
GGTATCA14600.019.0695781
TACCGTC7500.016.0016217
CTAGCGG7700.015.9994929
ATACCGT7850.015.2881736
TCTAGCG7900.015.18938828
ACCGTCG7600.015.15943058
CGTCGTA8350.014.37191610
GCATTCG10300.013.35879822
TAGGACG9600.013.3342414
TAGCGGC9250.013.31849430
TAAACGC4700.013.27617128
TATCAAC61550.013.1014172
TTTCGTT8550.013.09899728
TAATACC3950.012.9629074
CCGTCGT9050.012.9070989
CTTTCGT8900.012.76363827
TCGCGTA4150.012.7238979
GATATAC7950.012.6799671
TCAACGC62900.012.592274
Produced by FastQC (version 0.11.5)