##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062718_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4504610
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27040498511525	32.0	32.0	32.0	32.0	32.0
2	31.339074414877203	32.0	32.0	32.0	32.0	32.0
3	31.396569070352374	32.0	32.0	32.0	32.0	32.0
4	31.488518650893194	32.0	32.0	32.0	32.0	32.0
5	31.418901303331477	32.0	32.0	32.0	32.0	32.0
6	34.94979609777539	36.0	36.0	36.0	36.0	36.0
7	34.9788776386857	36.0	36.0	36.0	36.0	36.0
8	34.91144915986067	36.0	36.0	36.0	36.0	36.0
9	35.01822177724598	36.0	36.0	36.0	36.0	36.0
10	34.876495856467045	36.0	36.0	36.0	32.0	36.0
11	35.03996794395075	36.0	36.0	36.0	36.0	36.0
12	34.95163354874229	36.0	36.0	36.0	36.0	36.0
13	34.99969298119038	36.0	36.0	36.0	36.0	36.0
14	34.94331251762084	36.0	36.0	36.0	32.0	36.0
15	34.916995033976306	36.0	36.0	36.0	32.0	36.0
16	34.934994372431795	36.0	36.0	36.0	36.0	36.0
17	34.90751408002025	36.0	36.0	36.0	32.0	36.0
18	34.90050193024479	36.0	36.0	36.0	32.0	36.0
19	34.89708987015524	36.0	36.0	36.0	32.0	36.0
20	34.890152754622484	36.0	36.0	36.0	32.0	36.0
21	34.881591747121284	36.0	36.0	36.0	32.0	36.0
22	34.864085237123746	36.0	36.0	36.0	32.0	36.0
23	34.815824677386054	36.0	36.0	36.0	32.0	36.0
24	34.796306006513326	36.0	36.0	36.0	32.0	36.0
25	34.78445747800586	36.0	36.0	36.0	32.0	36.0
26	34.7188569043713	36.0	36.0	36.0	32.0	36.0
27	34.70011588128606	36.0	36.0	36.0	32.0	36.0
28	34.67732656101194	36.0	36.0	36.0	32.0	36.0
29	34.64636650009657	36.0	36.0	36.0	32.0	36.0
30	34.62849347668278	36.0	36.0	36.0	32.0	36.0
31	34.62227939821649	36.0	36.0	36.0	32.0	36.0
32	34.60443412415281	36.0	36.0	36.0	32.0	36.0
33	34.57947191876766	36.0	36.0	36.0	32.0	36.0
34	34.57721178969989	36.0	36.0	36.0	32.0	36.0
35	34.55501630551812	36.0	36.0	36.0	32.0	36.0
36	34.52957503535267	36.0	36.0	36.0	32.0	36.0
37	34.527744466224604	36.0	36.0	36.0	32.0	36.0
38	34.12937590601628	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	4.0
20	21.0
21	83.0
22	310.0
23	1074.0
24	2874.0
25	6798.0
26	14831.0
27	28653.0
28	49810.0
29	78315.0
30	118775.0
31	171265.0
32	245213.0
33	391322.0
34	984006.0
35	2411253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.330710968972326	18.185917381646703	12.237298257961173	27.246073391419795
2	15.824991691842733	20.122501198217204	36.79704498215051	27.25546212778956
3	17.844252887597374	23.789717644812757	28.883144156763844	29.482885310826017
4	12.342594097228696	16.511847011363507	35.66524056763359	35.480318323774206
5	14.336728747581642	36.68423757465971	33.759519425121624	15.219514252637023
6	34.70723260666874	35.60957687697021	16.5286151933579	13.154575323003154
7	30.443694295682533	30.269782719307514	20.75293012368723	18.533592861322724
8	28.05935550499398	33.50439858284536	18.971992348128623	19.46425356403203
9	27.42362875418143	14.078385700876733	17.997026936065804	40.50095860887603
10	15.597363476949738	27.046649746068933	31.986944810924197	25.369041966057132
11	37.53142782041169	20.851644259162605	22.327988670555186	19.288939249870516
12	24.51423534037103	23.349070583197307	29.085319248172816	23.05137482825885
13	29.81763748560083	19.503400857656807	24.815727215674467	25.863234441067895
14	23.536066385325256	19.21897789153778	25.003785011355035	32.24117071178193
15	25.288138151804485	27.306936671543152	21.78006531087042	25.624859865781946
16	25.829783266475896	25.517103589433937	23.650105114538217	25.00300802955195
17	24.33200654440673	25.71667691542664	24.931858695869344	25.01945784429729
18	24.93529961528301	25.214879867513503	25.57475563922293	24.275064877980558
19	25.71800888423193	24.887370937772637	24.93882933261703	24.4557908453784
20	25.93793913346549	24.618957024026496	24.503164535886572	24.93993930662144
21	26.748609423949876	24.28062552834786	24.1808166215573	24.78994842614496
22	26.031699095681578	24.448786664677595	24.634241203674105	24.885273035966726
23	25.120632135477393	24.31470113688129	24.908049630999752	25.656617096641572
24	25.331094145775108	24.761100295031092	24.72471534716657	25.18309021202724
25	25.35121281444211	24.45433062388378	24.915192342058848	25.27926421961526
26	24.95507931215294	25.19907401446431	25.13418487654636	24.711661796836392
27	25.48806144464989	24.712766285715933	24.560455551394384	25.238716718239797
28	25.03131236666437	24.76403062640273	24.936587185128126	25.268069821804772
29	24.896050934487114	24.692903492200212	25.02241037514901	25.388635198163662
30	24.94417940731828	25.03999236337885	25.031134770823666	24.9846934584792
31	25.477921507078307	24.62510628001092	24.573847680487322	25.323124532423453
32	25.156650631242215	24.893275999476092	24.36865788603231	25.58141548324938
33	24.964314335758257	24.658494298063538	24.797884833537196	25.579306532641006
34	25.46564519458954	24.51968094907217	25.088054237769754	24.926619618568534
35	25.573001880295966	24.567565227622367	24.977634023811163	24.881798868270504
36	24.85971038558277	24.89986924506228	24.818752344820084	25.421668024534867
37	25.498100834478453	24.80905117202155	24.556287891737576	25.136560101762417
38	25.085784398061804	24.724021429616393	24.780230550634048	25.409963621687755
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	50.0
2	85.0
3	85.0
4	295.5
5	506.0
6	506.0
7	679.0
8	852.0
9	865.0
10	878.0
11	878.0
12	1482.5
13	2087.0
14	3309.0
15	4531.0
16	4531.0
17	7255.0
18	9979.0
19	9979.0
20	13678.5
21	17378.0
22	21936.5
23	26495.0
24	26495.0
25	35395.5
26	44296.0
27	44296.0
28	57926.0
29	71556.0
30	89132.0
31	106708.0
32	106708.0
33	133079.0
34	159450.0
35	159450.0
36	178628.5
37	197807.0
38	223524.0
39	249241.0
40	249241.0
41	267365.0
42	285489.0
43	319097.5
44	352706.0
45	352706.0
46	364777.5
47	376849.0
48	376849.0
49	392442.5
50	408036.0
51	414826.5
52	421617.0
53	421617.0
54	414131.0
55	406645.0
56	406645.0
57	387861.5
58	369078.0
59	335690.0
60	302302.0
61	302302.0
62	275267.0
63	248232.0
64	208249.5
65	168267.0
66	168267.0
67	139953.5
68	111640.0
69	111640.0
70	90336.5
71	69033.0
72	54151.5
73	39270.0
74	39270.0
75	30626.0
76	21982.0
77	21982.0
78	18593.5
79	15205.0
80	11648.0
81	8091.0
82	8091.0
83	6765.5
84	5440.0
85	5440.0
86	3592.5
87	1745.0
88	1274.5
89	804.0
90	804.0
91	482.0
92	160.0
93	132.0
94	104.0
95	104.0
96	66.5
97	29.0
98	29.0
99	25.5
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008990789435711416
2	2.2199480088176335E-5
3	0.0
4	3.107927212344687E-4
5	1.1099740044088167E-4
6	2.2199480088176335E-4
7	4.883885619398794E-4
8	0.0020201526880240465
9	0.0036851136946372716
10	9.101786836152297E-4
11	0.005350074701250497
12	2.441942809699397E-4
13	2.441942809699397E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	4.439896017635267E-5
22	4.439896017635267E-5
23	1.33196880529058E-4
24	0.0
25	1.1099740044088167E-4
26	8.879792035270534E-5
27	6.6598440264529E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.6598440264529E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4504610.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.024242627291777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.71631142438918	16.428158098763177
2	15.940862720800519	9.572246600353306
3	8.5698884789732	7.7191323294457135
4	5.1846144865312285	6.226564930903415
5	3.5011113715497455	5.2559108642289925
6	2.4648950294799032	4.440396384954607
7	1.8446370826869591	3.876868193092505
8	1.3600352320391302	3.266722223072634
9	1.031187547399695	2.7864562605633343
>10	5.106495063586472	25.454824054035434
>50	0.16928283365541297	3.457817871597927
>100	0.09111724448077749	5.3972517831746405
>500	0.012867440766270964	2.682103894955512
>1k	0.006694043661443049	3.435546510858666
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4672	0.10371597097195984	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7759584070541068E-4
2	0.0	0.0	0.0	0.0	1.7759584070541068E-4
3	0.0	0.0	0.0	0.0	1.7759584070541068E-4
4	0.0	0.0	0.0	0.0	1.7759584070541068E-4
5	0.0	0.0	0.0	0.0	1.7759584070541068E-4
6	0.0	0.0	0.0	0.0	2.66393761058116E-4
7	0.0	0.0	0.0	0.0	2.66393761058116E-4
8	0.0	0.0	0.0	0.0	2.66393761058116E-4
9	0.0	0.0	0.0	2.2199480088176335E-5	2.66393761058116E-4
10	0.0	0.0	0.0	2.2199480088176335E-5	2.66393761058116E-4
11	0.0	0.0	0.0	2.2199480088176335E-5	2.66393761058116E-4
12	0.0	0.0	0.0	2.2199480088176335E-5	6.437849225571137E-4
13	0.0	0.0	0.0	2.2199480088176335E-5	8.213807632625244E-4
14	0.0	0.0	0.0	2.2199480088176335E-5	8.213807632625244E-4
15	0.0	0.0	0.0	4.439896017635267E-5	8.213807632625244E-4
16	0.0	0.0	0.0	4.439896017635267E-5	8.435802433507007E-4
17	0.0	0.0	0.0	4.439896017635267E-5	8.657797234388771E-4
18	0.0	0.0	0.0	8.879792035270534E-5	8.657797234388771E-4
19	0.0	0.0	0.0	8.879792035270534E-5	8.657797234388771E-4
20	0.0	0.0	0.0	1.1099740044088167E-4	9.767771238797587E-4
21	0.0	0.0	0.0	1.33196880529058E-4	0.0010211760840561114
22	0.0	0.0	0.0	1.33196880529058E-4	0.0011765724446733457
23	0.0	0.0	0.0	1.7759584070541068E-4	0.0011765724446733457
24	0.0	2.2199480088176335E-5	0.0	2.66393761058116E-4	0.001198771924761522
25	0.0	2.2199480088176335E-5	0.0	3.773911614989977E-4	0.001198771924761522
26	0.0	2.2199480088176335E-5	0.0	5.549870022044084E-4	0.0013097693252024038
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3990	0.0	20.211676	1
GGTATCA	1460	0.0	19.069578	1
TACCGTC	750	0.0	16.001621	7
CTAGCGG	770	0.0	15.99949	29
ATACCGT	785	0.0	15.288173	6
TCTAGCG	790	0.0	15.189388	28
ACCGTCG	760	0.0	15.1594305	8
CGTCGTA	835	0.0	14.371916	10
GCATTCG	1030	0.0	13.358798	22
TAGGACG	960	0.0	13.334241	4
TAGCGGC	925	0.0	13.318494	30
TAAACGC	470	0.0	13.276171	28
TATCAAC	6155	0.0	13.101417	2
TTTCGTT	855	0.0	13.098997	28
TAATACC	395	0.0	12.962907	4
CCGTCGT	905	0.0	12.907098	9
CTTTCGT	890	0.0	12.763638	27
TCGCGTA	415	0.0	12.723897	9
GATATAC	795	0.0	12.679967	1
TCAACGC	6290	0.0	12.59227	4
>>END_MODULE