FastQCFastQC Report
Thu 2 Feb 2017
SRR4062717_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062717_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3406755
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT45710.13417460310471402No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC41940.12310835384405396No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA41280.12117102638728057No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT38980.11441973373488848No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG37960.11142568221078415No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG37650.11051572537502698No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC37400.10978188921715827No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC36150.10611270842781474No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG36080.1059072343036115No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA35540.1043221482026151No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG34100.10009525193329136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT7500.019.1988229
TCTAGCG9450.017.60686728
CATCGTT8400.017.33175828
TAACGCT652.106221E-417.226174
CGTTTAT8500.016.75191131
CTAGCGG10050.016.7153929
TAGCGGC10100.016.31582830
CGGCATC9050.015.55751125
GCATCGT9200.015.47662727
TCGTTTA9250.015.39364730
AAGACGG10300.015.064165
TTTTCGG9750.014.76832229
GTATTAG8800.014.7415151
CAAGACG10100.014.5703674
CGGTCCA11850.014.44806610
GTATCAA51100.014.4170611
GTTTTCG10200.014.116364528
GGCATCG10100.013.93973726
CGCCGGT11950.013.9234217
AACCGCG6600.013.8169267
Produced by FastQC (version 0.11.5)