##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062717_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3406755
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259950011080925	32.0	32.0	32.0	32.0	32.0
2	30.882276242347924	32.0	32.0	32.0	32.0	32.0
3	30.930232728799105	32.0	32.0	32.0	32.0	32.0
4	31.00244161966446	32.0	32.0	32.0	32.0	32.0
5	30.904557269307595	32.0	32.0	32.0	32.0	32.0
6	34.55794619806825	36.0	36.0	36.0	32.0	36.0
7	34.50794260227108	36.0	36.0	36.0	32.0	36.0
8	34.48550013135667	36.0	36.0	36.0	32.0	36.0
9	34.63502394507383	36.0	36.0	36.0	32.0	36.0
10	34.34637771134114	36.0	36.0	36.0	32.0	36.0
11	34.60900710500168	36.0	36.0	36.0	32.0	36.0
12	34.43091563672762	36.0	36.0	36.0	32.0	36.0
13	34.51798412272089	36.0	36.0	36.0	32.0	36.0
14	34.41615437564486	36.0	36.0	36.0	32.0	36.0
15	34.36002911861875	36.0	36.0	36.0	32.0	36.0
16	34.37982713755465	36.0	36.0	36.0	32.0	36.0
17	34.30247816470512	36.0	36.0	36.0	32.0	36.0
18	34.32920535817809	36.0	36.0	36.0	32.0	36.0
19	34.32259408146462	36.0	36.0	36.0	32.0	36.0
20	34.30053731483479	36.0	36.0	36.0	32.0	36.0
21	34.289187511282734	36.0	36.0	36.0	32.0	36.0
22	34.26442435690268	36.0	36.0	36.0	32.0	36.0
23	34.21241474658436	36.0	36.0	36.0	32.0	36.0
24	34.191635148403684	36.0	36.0	36.0	32.0	36.0
25	34.1667448936011	36.0	36.0	36.0	32.0	36.0
26	34.12069109754003	36.0	36.0	36.0	32.0	36.0
27	34.11321712303938	36.0	36.0	36.0	32.0	36.0
28	34.094604689800114	36.0	36.0	36.0	32.0	36.0
29	34.07232806585739	36.0	36.0	36.0	32.0	36.0
30	34.04124159207222	36.0	36.0	36.0	32.0	36.0
31	34.057573849601745	36.0	36.0	36.0	32.0	36.0
32	34.01489746107366	36.0	36.0	36.0	32.0	36.0
33	33.98478728291292	36.0	36.0	36.0	32.0	36.0
34	33.995160203771626	36.0	36.0	36.0	32.0	36.0
35	33.9495895654369	36.0	36.0	36.0	32.0	36.0
36	33.91634355860636	36.0	36.0	36.0	32.0	36.0
37	33.908495327665186	36.0	36.0	36.0	32.0	36.0
38	33.30753165402267	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	6.0
8	5.0
9	16.0
10	32.0
11	35.0
12	16.0
13	13.0
14	244.0
15	643.0
16	922.0
17	1152.0
18	1407.0
19	1875.0
20	2640.0
21	3936.0
22	5966.0
23	9214.0
24	14210.0
25	21888.0
26	31557.0
27	45901.0
28	65146.0
29	88155.0
30	118954.0
31	160328.0
32	219062.0
33	329479.0
34	732685.0
35	1551265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.63769632276874	16.942654942161194	11.228516176055676	26.19113255901439
2	16.79515848695081	19.174122129199038	37.64058473617031	26.390134647679837
3	18.96696028452701	23.39715032637526	28.250725482223665	29.385163906874062
4	12.410832986709304	15.10522448761532	36.007754202125305	36.476188323550076
5	14.784321921459318	36.36991802143043	33.716798209668816	15.128961847441428
6	34.96080867712884	35.30210179851817	16.635067626056397	13.102021898296586
7	30.78065829838559	30.032203684857695	20.82657592141256	18.36056209534416
8	28.40663754986807	33.135323839014376	19.14854645454201	19.30949215657554
9	27.396729900027765	14.203964721760883	18.274623350258576	40.12468202795277
10	15.549684184695153	26.672670454845658	31.811163640996163	25.96648171946302
11	38.12940770692831	20.88141663058554	21.92116946876353	19.068006193722617
12	24.719894348160853	23.43999006651968	28.834274968950535	23.005840616368932
13	29.53402223113404	19.251931910326245	25.173022012122548	26.041023846417165
14	23.53294316718001	19.658485233142674	24.898485561934375	31.91008603774294
15	25.33094728126592	27.133698679205676	22.038870725989128	25.49648331353928
16	26.10371816915992	25.543672113687265	23.557162891290236	24.79544682586258
17	24.331982486257385	25.779217976688862	25.084182053434358	24.804617483619403
18	25.220517005524666	24.69479295707668	25.94227574019386	24.14241429720479
19	25.76315160638621	25.041846887654245	25.02614146156163	24.16886004439792
20	25.744868037773188	24.37427033799368	24.75797311233118	25.122888511901948
21	26.98218859394838	24.21452946918047	24.142579314697528	24.660702622173623
22	25.71220145135154	24.301272868180643	24.817201333928278	25.169324346539533
23	24.45493122514792	23.99984734639598	25.35949189835031	26.18572953010578
24	24.869370789670384	25.087061675071915	24.89232888439513	25.151238650862574
25	25.052192863603924	24.50564675259247	24.956101038220353	25.486059345583257
26	24.81109499286129	25.258325046673928	25.43771030425912	24.492869656205656
27	25.51038740829114	24.774573512695984	24.45755558895642	25.257483490056458
28	24.572786124608808	24.66739800308171	25.401924352181638	25.357891520127847
29	24.567626114493173	24.96197564033726	25.429423529864202	25.04097471530536
30	24.633576651763452	25.09091784385057	25.592173183778655	24.683332320607327
31	25.242305193728203	24.873750605818028	24.496330746259193	25.38761345419458
32	24.87371991610194	24.845509266801997	24.456489990503492	25.82428082659257
33	24.526786445568003	24.458181158469404	25.143353345410446	25.871679050552142
34	25.36083097691809	24.654346764117356	25.244546581867034	24.74027567709752
35	25.903125267896044	24.455517807086665	25.20923781887703	24.432119106140266
36	24.57771172836389	25.234307751385415	24.829164665416396	25.358815854834297
37	25.679901808677812	24.905949399529298	24.72757984870293	24.686568943089956
38	24.7705662550759	24.750105605718883	25.16565933928835	25.313668799916865
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	82.0
1	153.0
2	224.0
3	224.0
4	564.0
5	904.0
6	904.0
7	1069.0
8	1234.0
9	1328.0
10	1422.0
11	1422.0
12	1906.5
13	2391.0
14	3542.0
15	4693.0
16	4693.0
17	7144.5
18	9596.0
19	9596.0
20	12209.0
21	14822.0
22	17764.0
23	20706.0
24	20706.0
25	26701.0
26	32696.0
27	32696.0
28	42021.0
29	51346.0
30	63421.5
31	75497.0
32	75497.0
33	96093.0
34	116689.0
35	116689.0
36	131630.5
37	146572.0
38	166850.0
39	187128.0
40	187128.0
41	200951.0
42	214774.0
43	242569.5
44	270365.0
45	270365.0
46	279670.5
47	288976.0
48	288976.0
49	305754.0
50	322532.0
51	324262.0
52	325992.0
53	325992.0
54	310189.0
55	294386.0
56	294386.0
57	285834.5
58	277283.0
59	248951.5
60	220620.0
61	220620.0
62	206062.0
63	191504.0
64	159003.0
65	126502.0
66	126502.0
67	104405.0
68	82308.0
69	82308.0
70	65571.5
71	48835.0
72	38465.0
73	28095.0
74	28095.0
75	20971.0
76	13847.0
77	13847.0
78	13365.0
79	12883.0
80	10027.0
81	7171.0
82	7171.0
83	7161.0
84	7151.0
85	7151.0
86	4748.0
87	2345.0
88	2064.5
89	1784.0
90	1784.0
91	1288.0
92	792.0
93	684.0
94	576.0
95	576.0
96	552.0
97	528.0
98	528.0
99	1016.0
100	1504.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12313770729036869
2	0.054861591162264384
3	0.01174137852589928
4	0.002524396383068345
5	3.228879094622302E-4
6	1.761206778884892E-4
7	5.87068926294964E-5
8	4.696551410359712E-4
9	2.6418101683273377E-4
10	4.990085873507194E-4
11	0.002348275705179856
12	0.002759223953586331
13	0.015880214456278775
14	0.010068232085958632
15	0.02495042936753597
16	0.01256327502271223
17	0.02183896405817266
18	0.006310990957670863
19	0.008483145984962229
20	0.0062229306187266175
21	0.0067512926523920855
22	0.008072197736555755
23	0.01027370621016187
24	0.01611504202679676
25	0.01872749874880935
26	0.019901636601399278
27	0.009481163159663668
28	0.006046809940838129
29	0.011330430277492805
30	0.003170172201992806
31	0.005988103048208633
32	0.0070448271155395675
33	0.009158275250201438
34	0.013238404287951437
35	0.0171130592014982
36	0.01617374891942626
37	0.01009758553227338
38	0.006340344403985612
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3406755.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.12300444721786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.78195540764548	37.984192879162805
2	13.881572220555539	13.915722122905624
3	4.622811311453889	6.951275757679569
4	2.015235771591312	4.040386861666554
5	1.0256373827264238	2.5704013547814712
6	0.6283369647154478	1.8896481886070255
7	0.39991819824209346	1.4031571140308263
8	0.2864106621825644	1.1484610315445831
9	0.2078507212209578	0.9376292361703958
>10	0.9974885700902957	8.802711748328235
>50	0.07025570965788104	2.4507755981569863
>100	0.06294759216953705	6.713086066315645
>500	0.011676923418028033	4.045395676622023
>1k	0.00790256433058297	7.14715636402833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4571	0.13417460310471402	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	4194	0.12310835384405396	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	4128	0.12117102638728057	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3898	0.11441973373488848	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3796	0.11142568221078415	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3765	0.11051572537502698	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3740	0.10978188921715827	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3615	0.10611270842781474	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3608	0.1059072343036115	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3554	0.1043221482026151	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3410	0.10009525193329136	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.93534463147482E-5
2	0.0	0.0	0.0	0.0	5.87068926294964E-5
3	0.0	0.0	0.0	0.0	5.87068926294964E-5
4	0.0	0.0	0.0	0.0	5.87068926294964E-5
5	0.0	0.0	0.0	0.0	5.87068926294964E-5
6	0.0	0.0	0.0	0.0	8.80603389442446E-5
7	0.0	0.0	0.0	0.0	8.80603389442446E-5
8	0.0	0.0	0.0	2.93534463147482E-5	8.80603389442446E-5
9	0.0	0.0	0.0	5.87068926294964E-5	8.80603389442446E-5
10	0.0	0.0	0.0	8.80603389442446E-5	8.80603389442446E-5
11	0.0	2.93534463147482E-5	0.0	8.80603389442446E-5	8.80603389442446E-5
12	0.0	2.93534463147482E-5	0.0	8.80603389442446E-5	3.815948020917266E-4
13	0.0	2.93534463147482E-5	0.0	8.80603389442446E-5	4.990085873507194E-4
14	0.0	2.93534463147482E-5	0.0	8.80603389442446E-5	4.990085873507194E-4
15	2.93534463147482E-5	2.93534463147482E-5	0.0	1.46767231573741E-4	6.751292652392085E-4
16	2.93534463147482E-5	2.93534463147482E-5	0.0	2.0547412420323738E-4	7.044827115539568E-4
17	2.93534463147482E-5	2.93534463147482E-5	0.0	2.348275705179856E-4	7.33836157868705E-4
18	2.93534463147482E-5	2.93534463147482E-5	0.0	2.6418101683273377E-4	8.512499431276978E-4
19	2.93534463147482E-5	2.93534463147482E-5	0.0	3.815948020917266E-4	8.512499431276978E-4
20	2.93534463147482E-5	2.93534463147482E-5	0.0	5.283620336654675E-4	9.099568357571942E-4
21	2.93534463147482E-5	5.87068926294964E-5	0.0	6.164223726097122E-4	9.393102820719424E-4
22	2.93534463147482E-5	5.87068926294964E-5	0.0	8.512499431276978E-4	9.686637283866905E-4
23	2.93534463147482E-5	5.87068926294964E-5	0.0	0.0012915516378489208	0.001027370621016187
24	2.93534463147482E-5	5.87068926294964E-5	0.0	0.0017318533325701437	0.001027370621016187
25	5.87068926294964E-5	5.87068926294964E-5	0.0	0.0019960343494028776	0.001027370621016187
26	5.87068926294964E-5	5.87068926294964E-5	0.0	0.0022895688125503595	0.0011154309599604316
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	750	0.0	19.19882	29
TCTAGCG	945	0.0	17.606867	28
CATCGTT	840	0.0	17.331758	28
TAACGCT	65	2.106221E-4	17.22617	4
CGTTTAT	850	0.0	16.751911	31
CTAGCGG	1005	0.0	16.71539	29
TAGCGGC	1010	0.0	16.315828	30
CGGCATC	905	0.0	15.557511	25
GCATCGT	920	0.0	15.476627	27
TCGTTTA	925	0.0	15.393647	30
AAGACGG	1030	0.0	15.06416	5
TTTTCGG	975	0.0	14.768322	29
GTATTAG	880	0.0	14.741515	1
CAAGACG	1010	0.0	14.570367	4
CGGTCCA	1185	0.0	14.448066	10
GTATCAA	5110	0.0	14.417061	1
GTTTTCG	1020	0.0	14.1163645	28
GGCATCG	1010	0.0	13.939737	26
CGCCGGT	1195	0.0	13.923421	7
AACCGCG	660	0.0	13.816926	7
>>END_MODULE