FastQCFastQC Report
Thu 2 Feb 2017
SRR4062717_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062717_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3406755
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC47020.13801990457194602No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46410.13622934434674638No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA41210.12096555226307733No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC39680.11647447497692086No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG39020.11453714752014749No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA38660.11348042345281655No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT38440.11283464763389207No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG37810.11098538051606294No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC37430.1098699495561025No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG36210.10628882910570323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT9000.016.71068229
CTAGCGG8650.016.46200629
CGTTTAT9300.016.17162731
TCTAGCG8950.015.91020528
CGGCATC9700.015.83464425
CATCGTT9800.015.67306828
ACCGTCG6950.015.4251978
GCATCGT10050.015.2831927
CGTCGTA6800.015.05865810
GTTTTCG10000.015.03961428
TCGTTTA10150.014.97498430
TTTTCGG10000.014.87961829
ATACCGT7350.014.8036446
GGCATCG10500.014.78057326
TAGCGGC9700.014.68003530
TACCGTC7150.014.5463637
GTATCAA48750.014.5079981
CGCGTAA4550.014.41742410
GTATTAG8550.014.2235271
GCGTAAC4750.014.14721311
Produced by FastQC (version 0.11.5)