##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062717_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3406755
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19120746869088	32.0	32.0	32.0	32.0	32.0
2	31.227078847759817	32.0	32.0	32.0	32.0	32.0
3	31.341413750034857	32.0	32.0	32.0	32.0	32.0
4	31.450077272947425	32.0	32.0	32.0	32.0	32.0
5	31.358210379085083	32.0	32.0	32.0	32.0	32.0
6	34.88091541657677	36.0	36.0	36.0	36.0	36.0
7	34.92032506006449	36.0	36.0	36.0	36.0	36.0
8	34.84262912947952	36.0	36.0	36.0	32.0	36.0
9	34.97646587441715	36.0	36.0	36.0	36.0	36.0
10	34.80330519805504	36.0	36.0	36.0	32.0	36.0
11	35.00781799689147	36.0	36.0	36.0	36.0	36.0
12	34.891501736990186	36.0	36.0	36.0	32.0	36.0
13	34.954854986636846	36.0	36.0	36.0	36.0	36.0
14	34.89423659758333	36.0	36.0	36.0	32.0	36.0
15	34.86112561660583	36.0	36.0	36.0	32.0	36.0
16	34.88051386143118	36.0	36.0	36.0	32.0	36.0
17	34.84180723298271	36.0	36.0	36.0	32.0	36.0
18	34.84608902019664	36.0	36.0	36.0	32.0	36.0
19	34.841896467459506	36.0	36.0	36.0	32.0	36.0
20	34.83531836014037	36.0	36.0	36.0	32.0	36.0
21	34.8272916602456	36.0	36.0	36.0	32.0	36.0
22	34.80368561871928	36.0	36.0	36.0	32.0	36.0
23	34.74306047837312	36.0	36.0	36.0	32.0	36.0
24	34.717355958969755	36.0	36.0	36.0	32.0	36.0
25	34.700078814003355	36.0	36.0	36.0	32.0	36.0
26	34.63422641193746	36.0	36.0	36.0	32.0	36.0
27	34.61687265447618	36.0	36.0	36.0	32.0	36.0
28	34.58660837072229	36.0	36.0	36.0	32.0	36.0
29	34.553916263423694	36.0	36.0	36.0	32.0	36.0
30	34.53022304216182	36.0	36.0	36.0	32.0	36.0
31	34.53245008813372	36.0	36.0	36.0	32.0	36.0
32	34.50698802819692	36.0	36.0	36.0	32.0	36.0
33	34.47370444895509	36.0	36.0	36.0	32.0	36.0
34	34.46624515117759	36.0	36.0	36.0	32.0	36.0
35	34.44518640172246	36.0	36.0	36.0	32.0	36.0
36	34.4088882235441	36.0	36.0	36.0	32.0	36.0
37	34.40748923829275	36.0	36.0	36.0	32.0	36.0
38	33.95750031921873	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	12.0
21	69.0
22	243.0
23	793.0
24	2209.0
25	5448.0
26	11648.0
27	22731.0
28	38842.0
29	62646.0
30	95065.0
31	138878.0
32	201545.0
33	326421.0
34	794398.0
35	1705806.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96111396028876	18.168111527544436	12.002174703745053	26.86859980842175
2	15.80339648727308	20.157657360156513	36.869895252226826	27.169050900343585
3	17.892569321832653	24.148052912522328	28.59621546016664	29.36316230547838
4	11.91111413778113	15.979594634301128	36.01187759246273	36.09741363545501
5	14.284519179711108	36.89368550856813	33.31822607522534	15.503569236495418
6	35.122955824198534	35.68903753487193	16.224515315467634	12.963491325461906
7	30.59179743684578	30.23876198359722	20.703458438272364	18.465982141284627
8	28.254091914742226	33.43468391446512	18.874603460225085	19.436620710567574
9	27.279801704556462	13.987149778931343	18.0281474323335	40.704901084178694
10	15.285147173302743	26.94688047078549	31.78852930022086	25.979443055690908
11	38.27859682680027	20.784578609772286	21.78102872870343	19.155795834724017
12	24.49442239180074	23.4898570395747	28.823889769331274	23.191830799293285
13	29.595781784921638	19.166542648137565	25.128795810903593	26.108879756037208
14	23.42111481453759	19.5937776564502	24.851302779331064	32.13380474968115
15	25.329558480137255	27.194969993439503	21.87160509047466	25.60386643594858
16	26.115445964105422	25.602171451181977	23.415984834831043	24.866397749881557
17	24.2905051874878	25.855131936402824	24.91632066291823	24.938042213191146
18	25.295713956536353	24.652433180548645	25.81083758591387	24.241015277001136
19	25.70774828245647	25.076942721152534	24.948991048666546	24.266317947724446
20	25.75897004627571	24.32578802995813	24.71886002955892	25.196381894207242
21	27.01334352487682	24.24639230514984	24.041563415646486	24.698700754326854
22	25.66927344915424	24.371087551375883	24.756439707710037	25.203199291759837
23	24.436082611310667	23.972708291688058	25.26018206800677	26.3310270289945
24	24.9323769980524	25.065935178784503	24.78073709439041	25.22095072877269
25	25.11455185786822	24.47705352367679	24.92304991789839	25.4853447005566
26	24.940111595997713	25.18048710898618	25.25912503782928	24.62027625718683
27	25.59749743964264	24.699867997396932	24.40692025921472	25.295714303745708
28	24.59924473582632	24.61544783819206	25.330908738667734	25.45439868731388
29	24.659595421449442	24.777743042866305	25.356504943854198	25.20615659183005
30	24.634615638635594	24.94902040211286	25.56696915393094	24.849394805320603
31	25.262251027737538	24.77950424964519	24.48461952796723	25.47362519465004
32	24.96155432368926	24.739055200623465	24.46650845159103	25.832882024096243
33	24.549960299463862	24.35282255401401	25.16864288743981	25.92857425908232
34	25.3043732231992	24.532025343765547	25.294686585915336	24.868914847119914
35	25.89942628689178	24.43486543646373	25.143340216716492	24.522368059927995
36	24.747508993162114	25.16359409467367	24.740845760848668	25.34805115131555
37	25.73341074817847	24.902796033995998	24.643340845860898	24.720452371964633
38	24.858288899553973	24.548521980594437	25.18745844652756	25.40573067332403
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	56.0
2	83.0
3	83.0
4	371.5
5	660.0
6	660.0
7	828.0
8	996.0
9	1184.0
10	1372.0
11	1372.0
12	1862.5
13	2353.0
14	3422.0
15	4491.0
16	4491.0
17	7055.5
18	9620.0
19	9620.0
20	12105.5
21	14591.0
22	17812.0
23	21033.0
24	21033.0
25	26614.0
26	32195.0
27	32195.0
28	41654.5
29	51114.0
30	63123.0
31	75132.0
32	75132.0
33	96683.5
34	118235.0
35	118235.0
36	132233.0
37	146231.0
38	166364.5
39	186498.0
40	186498.0
41	199938.5
42	213379.0
43	241105.5
44	268832.0
45	268832.0
46	279140.5
47	289449.0
48	289449.0
49	306567.0
50	323685.0
51	323738.5
52	323792.0
53	323792.0
54	309468.0
55	295144.0
56	295144.0
57	287544.5
58	279945.0
59	252042.0
60	224139.0
61	224139.0
62	208730.5
63	193322.0
64	160253.0
65	127184.0
66	127184.0
67	105378.0
68	83572.0
69	83572.0
70	66359.5
71	49147.0
72	38650.5
73	28154.0
74	28154.0
75	20858.5
76	13563.0
77	13563.0
78	13083.5
79	12604.0
80	9831.0
81	7058.0
82	7058.0
83	6539.0
84	6020.0
85	6020.0
86	3842.0
87	1664.0
88	1386.0
89	1108.0
90	1108.0
91	651.5
92	195.0
93	149.5
94	104.0
95	104.0
96	64.0
97	24.0
98	24.0
99	31.0
100	38.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009216982142830935
2	0.0
3	0.0
4	1.174137852589928E-4
5	1.174137852589928E-4
6	8.80603389442446E-5
7	4.4030169472122297E-4
8	0.0028766377388453235
9	0.003551767004084532
10	6.751292652392085E-4
11	0.004755258302989208
12	2.348275705179856E-4
13	1.761206778884892E-4
14	0.0
15	0.0
16	2.93534463147482E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	2.6418101683273377E-4
22	2.93534463147482E-5
23	1.761206778884892E-4
24	0.0
25	2.93534463147482E-5
26	5.87068926294964E-5
27	1.174137852589928E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	2.93534463147482E-5
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3406755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.89636669911832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.50908294657155	35.208179925248956
2	15.124100374656162	14.487789150776084
3	5.098525566920352	7.326025505341419
4	2.1948872911993424	4.205085062500728
5	1.151591839303675	2.7578532511500478
6	0.6874444235417354	1.9755654117131378
7	0.4551487632242964	1.5259980446228756
8	0.3190385360938716	1.2224629372721574
9	0.22334492686997975	0.962766946597708
>10	1.0726658358359966	9.075967073361767
>50	0.074836349048773	2.504603403158465
>100	0.06786913989229876	6.848727811247432
>500	0.013014096103579183	4.353280829447171
>1k	0.008449910738388886	7.545694647562226
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	4702	0.13801990457194602	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4641	0.13622934434674638	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	4121	0.12096555226307733	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3968	0.11647447497692086	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3902	0.11453714752014749	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3866	0.11348042345281655	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3844	0.11283464763389207	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3781	0.11098538051606294	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3743	0.1098699495561025	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3621	0.10628882910570323	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.46767231573741E-4
2	0.0	0.0	0.0	0.0	1.46767231573741E-4
3	0.0	0.0	0.0	0.0	1.46767231573741E-4
4	0.0	0.0	0.0	0.0	1.46767231573741E-4
5	0.0	0.0	0.0	0.0	1.761206778884892E-4
6	0.0	0.0	0.0	0.0	2.348275705179856E-4
7	0.0	0.0	0.0	0.0	2.6418101683273377E-4
8	0.0	0.0	0.0	0.0	2.6418101683273377E-4
9	0.0	0.0	0.0	2.93534463147482E-5	2.6418101683273377E-4
10	0.0	0.0	2.93534463147482E-5	2.93534463147482E-5	2.93534463147482E-4
11	0.0	0.0	2.93534463147482E-5	2.93534463147482E-5	2.93534463147482E-4
12	0.0	0.0	2.93534463147482E-5	2.93534463147482E-5	5.283620336654675E-4
13	0.0	0.0	2.93534463147482E-5	2.93534463147482E-5	5.577154799802158E-4
14	0.0	0.0	2.93534463147482E-5	2.93534463147482E-5	7.044827115539568E-4
15	0.0	0.0	2.93534463147482E-5	8.80603389442446E-5	7.631896041834532E-4
16	0.0	0.0	2.93534463147482E-5	1.761206778884892E-4	8.218964968129495E-4
17	5.87068926294964E-5	0.0	2.93534463147482E-5	2.0547412420323738E-4	8.512499431276978E-4
18	5.87068926294964E-5	0.0	2.93534463147482E-5	2.0547412420323738E-4	8.512499431276978E-4
19	5.87068926294964E-5	0.0	2.93534463147482E-5	3.522413557769784E-4	8.512499431276978E-4
20	5.87068926294964E-5	0.0	2.93534463147482E-5	4.990085873507194E-4	9.686637283866905E-4
21	5.87068926294964E-5	0.0	2.93534463147482E-5	5.87068926294964E-4	0.001027370621016187
22	5.87068926294964E-5	0.0	2.93534463147482E-5	7.631896041834532E-4	0.001027370621016187
23	5.87068926294964E-5	0.0	2.93534463147482E-5	0.0010860775136456835	0.001056724067330935
24	5.87068926294964E-5	0.0	2.93534463147482E-5	0.0014089654231079136	0.001056724067330935
25	8.80603389442446E-5	0.0	2.93534463147482E-5	0.0017024998862553956	0.001056724067330935
26	8.80603389442446E-5	0.0	2.93534463147482E-5	0.001937327456773381	0.0011154309599604316
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	900	0.0	16.710682	29
CTAGCGG	865	0.0	16.462006	29
CGTTTAT	930	0.0	16.171627	31
TCTAGCG	895	0.0	15.910205	28
CGGCATC	970	0.0	15.834644	25
CATCGTT	980	0.0	15.673068	28
ACCGTCG	695	0.0	15.425197	8
GCATCGT	1005	0.0	15.28319	27
CGTCGTA	680	0.0	15.058658	10
GTTTTCG	1000	0.0	15.039614	28
TCGTTTA	1015	0.0	14.974984	30
TTTTCGG	1000	0.0	14.879618	29
ATACCGT	735	0.0	14.803644	6
GGCATCG	1050	0.0	14.780573	26
TAGCGGC	970	0.0	14.680035	30
TACCGTC	715	0.0	14.546363	7
GTATCAA	4875	0.0	14.507998	1
CGCGTAA	455	0.0	14.417424	10
GTATTAG	855	0.0	14.223527	1
GCGTAAC	475	0.0	14.147213	11
>>END_MODULE