FastQCFastQC Report
Thu 2 Feb 2017
SRR4062716_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062716_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796137
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG38780.4871020942375496No Hit
TCCATGTACTCTGCGTTGATACCAC31210.3920179567084559No Hit
GTATCAACGCAGAGTACTTTTTTTT19920.2502081928110363No Hit
GAGTACATGGAAGCAGTGGTATCAA17900.22483567526694528No Hit
CATGTACTCTGCGTTGATACCACTG15580.19569496204798922No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13860.1740906401787632No Hit
TATCAACGCAGAGTACTTTTTTTTT13470.1691919858014387No Hit
GCTTCCATGTACTCTGCGTTGATAC11480.14419628782483415No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10840.13615747038512216No Hit
GTCTTGCGCCGGTCCAAGAATTTCA10120.12711380076544615No Hit
CCCATGTACTCTGCGTTGATACCAC9840.12359681813557215No Hit
GTACATGGGAAGCAGTGGTATCAAC9460.11882377028074313No Hit
GCGCAAGACGGACCAGAGCGAAAGC9340.11731649201079714No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG9240.11606042678584214No Hit
CATGGAAGCAGTGGTATCAACGCAG8970.11266905067846363No Hit
GAATAGGACCGCGGTTCTATTTTGT8860.11128737893101312No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8570.10764478977864363No Hit
GCGTTGATACCACTGCTTCCATGTA8240.10349977453629212No Hit
GAATAACGCCGCCGCATCGCCAGTC8060.10123885713137312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG401.2889126E-518.9914531
GCGTTAT551.1317434E-515.5384631
GAAAGCG400.00527425114.2507549
TAGGCTG602.5653912E-514.249865
TAGGACT602.5653912E-514.249864
CATCGCC1350.014.07481915
CGCCAGT1301.8189894E-1213.88622418
CGACCAT903.6274287E-813.72294910
ATTTGCG707.243216E-613.57316
CGGACAT707.252631E-613.5712945
TAGAGTG1205.0931703E-1113.45825
TAAACTG500.001498537513.2998675
CGTTATT655.4625547E-513.1495822
AGAGTGT957.363815E-813.0006896
TTGCGCG751.4746776E-512.66813418
TTTGCGC751.4746776E-512.66813417
AACCGCG751.4756315E-512.6673377
CAAGACG1800.012.666544
GTCCTAA604.103671E-412.6609691
CGCATCG1457.2759576E-1212.44815313
Produced by FastQC (version 0.11.5)