##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062716_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	796137
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.147933081869077	32.0	32.0	32.0	32.0	32.0
2	30.774573974077327	32.0	32.0	32.0	32.0	32.0
3	30.762339898786266	32.0	32.0	32.0	32.0	32.0
4	30.848249735912287	32.0	32.0	32.0	32.0	32.0
5	30.61145631970377	32.0	32.0	32.0	32.0	32.0
6	34.24842081199593	36.0	36.0	36.0	32.0	36.0
7	34.13442535643991	36.0	36.0	36.0	32.0	36.0
8	34.11530804371609	36.0	36.0	36.0	32.0	36.0
9	34.32473807899897	36.0	36.0	36.0	32.0	36.0
10	33.9559736578001	36.0	36.0	36.0	32.0	36.0
11	34.30183498568714	36.0	36.0	36.0	32.0	36.0
12	34.073101740027155	36.0	36.0	36.0	32.0	36.0
13	34.20039390205454	36.0	36.0	36.0	32.0	36.0
14	34.0775281138799	36.0	36.0	36.0	32.0	36.0
15	34.012682490576374	36.0	36.0	36.0	32.0	36.0
16	34.01212982187739	36.0	36.0	36.0	32.0	36.0
17	33.92225207470574	36.0	36.0	36.0	32.0	36.0
18	33.93613159544149	36.0	36.0	36.0	32.0	36.0
19	33.94630069950272	36.0	36.0	36.0	32.0	36.0
20	33.93742534262319	36.0	36.0	36.0	32.0	36.0
21	33.90029605457352	36.0	36.0	36.0	32.0	36.0
22	33.84662564357642	36.0	36.0	36.0	32.0	36.0
23	33.79389728149803	36.0	36.0	36.0	32.0	36.0
24	33.757409842778316	36.0	36.0	36.0	32.0	36.0
25	33.25550878806035	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	4.0
4	28.0
5	71.0
6	132.0
7	30.0
8	116.0
9	153.0
10	102.0
11	33.0
12	50.0
13	39.0
14	97.0
15	128.0
16	251.0
17	287.0
18	439.0
19	585.0
20	978.0
21	1418.0
22	2205.0
23	3435.0
24	5008.0
25	6994.0
26	9830.0
27	12925.0
28	17372.0
29	23529.0
30	30893.0
31	42091.0
32	61217.0
33	87107.0
34	182978.0
35	305610.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.52266158176974	17.143061715926606	10.842336791515457	25.491939910788197
2	16.60501263018004	19.930813494132085	36.817194630383526	26.646979245304347
3	19.497198212785094	23.867760165507054	28.198126112455864	28.436915509251993
4	12.26459536973178	14.87067584730592	35.65613053894172	37.208598244020585
5	14.168338864045014	36.970367866669854	33.77281654162358	15.08847672766156
6	34.687743081866515	34.822580868114066	16.802195606524993	13.687480443494424
7	30.135782777721214	30.662549066187033	20.79438518166587	18.40728297442588
8	28.69217249624729	32.60743299816702	19.65349385109363	19.046900654492056
9	26.977269898425526	14.173278835068151	18.69075922487072	40.158692041635604
10	15.50027602042677	27.042205346622556	32.11142422767724	25.346094405273433
11	37.80567551049665	21.241917188753607	21.368802549276538	19.583604751473203
12	23.839151410877516	23.986930077423445	29.299290159324272	22.87462835237477
13	29.690783359720722	19.294444311704055	25.53976783286783	25.47500449570739
14	23.21462705487032	19.541263405702328	25.151159983098253	32.0929495563291
15	25.326593356847805	26.82736983734777	22.984451528126197	24.86158527767823
16	25.914604243632915	25.81991846712153	24.19253026046762	24.07294702877794
17	23.810344220486222	25.892744860334915	25.970085061645797	24.32682585753306
18	25.114415972331393	24.496777236283602	26.808740764030816	23.580066027354192
19	24.874988052178026	26.22760726628803	25.367364084092543	23.530040597441406
20	24.763986332774554	24.398286189286296	26.320098291345307	24.517629186593844
21	26.02771426487271	24.556855998007833	24.642504093752592	24.772925643366865
22	24.759017579169157	24.954243844146042	25.484197616277243	24.80254096040756
23	23.851102767767863	24.39685803948204	25.599934594897146	26.152104597852954
24	23.980897687925975	25.97262941036641	25.330812854442343	24.715660047265267
25	24.677368032488957	24.80250936673454	25.03694499189411	25.483177608882396
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	72.0
1	72.0
2	248.5
3	425.0
4	425.0
5	425.0
6	1282.5
7	2140.0
8	2140.0
9	2140.0
10	2203.0
11	2266.0
12	2266.0
13	2266.0
14	2310.5
15	2355.0
16	2355.0
17	2355.0
18	3844.0
19	5333.0
20	5333.0
21	5333.0
22	8383.0
23	11433.0
24	11433.0
25	11433.0
26	17284.5
27	23136.0
28	23136.0
29	23136.0
30	28876.0
31	34616.0
32	34616.0
33	34616.0
34	41762.0
35	48908.0
36	48908.0
37	48908.0
38	56099.0
39	63290.0
40	63290.0
41	63290.0
42	73288.0
43	83286.0
44	83286.0
45	83286.0
46	99614.0
47	115942.0
48	115942.0
49	115942.0
50	112174.0
51	108406.0
52	108406.0
53	108406.0
54	98270.0
55	88134.0
56	88134.0
57	88134.0
58	80858.5
59	73583.0
60	73583.0
61	73583.0
62	64561.0
63	55539.0
64	55539.0
65	55539.0
66	46280.0
67	37021.0
68	37021.0
69	37021.0
70	27729.5
71	18438.0
72	18438.0
73	18438.0
74	14426.0
75	10414.0
76	10414.0
77	10414.0
78	8610.0
79	6806.0
80	6806.0
81	6806.0
82	4624.0
83	2442.0
84	2442.0
85	2442.0
86	1845.0
87	1248.0
88	1248.0
89	1248.0
90	841.0
91	434.0
92	434.0
93	434.0
94	259.5
95	85.0
96	85.0
97	85.0
98	235.0
99	385.0
100	385.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006782752214757008
2	0.0035169826298740037
3	0.004773047854829006
4	0.014319143564487017
5	0.03491861325374904
6	0.04597198723335305
7	0.06632024387762409
8	0.0893062374943006
9	0.09546095709658012
10	0.11379950938092313
11	0.11693967244331065
12	0.12924911164786965
13	0.11731649201079714
14	0.11970301593821164
15	0.11078495284103113
16	0.10990570718356263
17	0.11920058984822965
18	0.12711380076544615
19	0.12723940728794164
20	0.11932619637072513
21	0.12849547251289664
22	0.14595477913977117
23	0.13716232256508618
24	0.13188684862027517
25	0.13012835730533814
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	796137.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.43751669925516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.82066026129188	44.25059186180942
2	12.78432288155075	14.174622264692793
3	3.573399710324037	5.943012183426071
4	1.3580156396804575	3.0114005881054022
5	0.6487758650299877	1.7983261425836827
6	0.3844684715407593	1.2788386386826773
7	0.2321316020773262	0.9008159696610639
8	0.15825542547100982	0.7018630233837471
9	0.11701379758292318	0.583825892179191
>10	0.7163728920079586	7.715825866232673
>50	0.10091109824779673	3.939986859732478
>100	0.09614823734591198	10.54264620360653
>500	0.00725647074221584	2.8556902465422187
>1k	0.00226764710694245	2.3025542593620036
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3878	0.4871020942375496	No Hit
TCCATGTACTCTGCGTTGATACCAC	3121	0.3920179567084559	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1992	0.2502081928110363	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1790	0.22483567526694528	No Hit
CATGTACTCTGCGTTGATACCACTG	1558	0.19569496204798922	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1386	0.1740906401787632	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1347	0.1691919858014387	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1148	0.14419628782483415	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1084	0.13615747038512216	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1012	0.12711380076544615	No Hit
CCCATGTACTCTGCGTTGATACCAC	984	0.12359681813557215	No Hit
GTACATGGGAAGCAGTGGTATCAAC	946	0.11882377028074313	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	934	0.11731649201079714	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	924	0.11606042678584214	No Hit
CATGGAAGCAGTGGTATCAACGCAG	897	0.11266905067846363	No Hit
GAATAGGACCGCGGTTCTATTTTGT	886	0.11128737893101312	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	857	0.10764478977864363	No Hit
GCGTTGATACCACTGCTTCCATGTA	824	0.10349977453629212	No Hit
GAATAACGCCGCCGCATCGCCAGTC	806	0.10123885713137312	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.2560652249550016E-4	0.0
10	0.0	0.0	0.0	1.2560652249550016E-4	0.0
11	0.0	0.0	0.0	2.512130449910003E-4	0.0
12	0.0	0.0	0.0	2.512130449910003E-4	0.0
13	0.0	0.0	0.0	2.512130449910003E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCTAG	40	1.2889126E-5	18.991453	1
GCGTTAT	55	1.1317434E-5	15.538463	1
GAAAGCG	40	0.005274251	14.250754	9
TAGGCTG	60	2.5653912E-5	14.24986	5
TAGGACT	60	2.5653912E-5	14.24986	4
CATCGCC	135	0.0	14.074819	15
CGCCAGT	130	1.8189894E-12	13.886224	18
CGACCAT	90	3.6274287E-8	13.722949	10
ATTTGCG	70	7.243216E-6	13.573	16
CGGACAT	70	7.252631E-6	13.571294	5
TAGAGTG	120	5.0931703E-11	13.4582	5
TAAACTG	50	0.0014985375	13.299867	5
CGTTATT	65	5.4625547E-5	13.149582	2
AGAGTGT	95	7.363815E-8	13.000689	6
TTGCGCG	75	1.4746776E-5	12.668134	18
TTTGCGC	75	1.4746776E-5	12.668134	17
AACCGCG	75	1.4756315E-5	12.667337	7
CAAGACG	180	0.0	12.66654	4
GTCCTAA	60	4.103671E-4	12.660969	1
CGCATCG	145	7.2759576E-12	12.448153	13
>>END_MODULE