FastQCFastQC Report
Thu 2 Feb 2017
SRR4062716_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062716_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796137
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA12930.1624092335866817No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC11720.14721084436472617No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC10220.12836986599040115No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8900.11178980502099511No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8800.11053373979604013No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8530.10714236368866163No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8030.10086203756388662No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA8020.10073643104139111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAAAC353.217045E-431.428328
CGCAATA2250.021.5109336
TCTAGCG2450.020.65288428
CCGGTCC2900.019.7239699
CGGTCCA2600.019.46137210
CTAGCGG2600.019.46137229
TAGCGGC2650.019.09417730
ATATATC1404.0727173E-918.8581663
ATACGAA2800.018.85698140
CGTCTAT600.00739956918.3377841
CCCTATA600.00740761718.3343282
AGTAAGG600.00740761718.3343282
GATTGCG600.007410301418.33317612
CGAATGC2650.018.26399443
GTCTAGG1355.5031705E-817.9302791
GCGCAAT2700.017.92577235
CAATACG2850.017.75423238
CCCCTAT750.001288247317.6042731
GCATTAG1002.4761164E-517.6042731
ATATACT1254.8477705E-717.599854
Produced by FastQC (version 0.11.5)