##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062716_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	796137
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.180943732046117	32.0	32.0	32.0	32.0	32.0
2	31.30831000192178	32.0	32.0	32.0	32.0	32.0
3	31.44626490164381	32.0	32.0	32.0	32.0	32.0
4	31.580783206910368	32.0	32.0	32.0	32.0	32.0
5	31.484378944829846	32.0	32.0	32.0	32.0	32.0
6	35.087955967377475	36.0	36.0	36.0	36.0	36.0
7	35.09217383440287	36.0	36.0	36.0	36.0	36.0
8	35.03345780939713	36.0	36.0	36.0	36.0	36.0
9	35.18026168862897	36.0	36.0	36.0	36.0	36.0
10	35.039157833387975	36.0	36.0	36.0	36.0	36.0
11	35.187011783147874	36.0	36.0	36.0	36.0	36.0
12	35.083641383329756	36.0	36.0	36.0	36.0	36.0
13	35.13756175130662	36.0	36.0	36.0	36.0	36.0
14	35.09511051489882	36.0	36.0	36.0	36.0	36.0
15	35.05118717004736	36.0	36.0	36.0	36.0	36.0
16	35.05636215877418	36.0	36.0	36.0	36.0	36.0
17	35.02815721414782	36.0	36.0	36.0	36.0	36.0
18	35.04845774031354	36.0	36.0	36.0	36.0	36.0
19	35.02084440240813	36.0	36.0	36.0	36.0	36.0
20	35.01534534885327	36.0	36.0	36.0	36.0	36.0
21	35.00071093291732	36.0	36.0	36.0	36.0	36.0
22	34.978905640612105	36.0	36.0	36.0	36.0	36.0
23	34.92066315219617	36.0	36.0	36.0	32.0	36.0
24	34.89939043154633	36.0	36.0	36.0	32.0	36.0
25	34.85858087238754	36.0	36.0	36.0	32.0	36.0
26	34.816084417631636	36.0	36.0	36.0	32.0	36.0
27	34.79635037688237	36.0	36.0	36.0	32.0	36.0
28	34.76829867221345	36.0	36.0	36.0	32.0	36.0
29	34.734222878725646	36.0	36.0	36.0	32.0	36.0
30	34.718030941910754	36.0	36.0	36.0	32.0	36.0
31	34.70225853088099	36.0	36.0	36.0	32.0	36.0
32	34.66681488236321	36.0	36.0	36.0	32.0	36.0
33	34.64002049898447	36.0	36.0	36.0	32.0	36.0
34	34.6167933408446	36.0	36.0	36.0	32.0	36.0
35	34.57713433743187	36.0	36.0	36.0	32.0	36.0
36	34.54805265927849	36.0	36.0	36.0	32.0	36.0
37	34.51613604191238	36.0	36.0	36.0	32.0	36.0
38	34.46781772483882	36.0	36.0	36.0	32.0	36.0
39	34.416022619222574	36.0	36.0	36.0	32.0	36.0
40	34.38061539659631	36.0	36.0	36.0	32.0	36.0
41	34.349290385951164	36.0	36.0	36.0	32.0	36.0
42	34.2776481937154	36.0	36.0	36.0	32.0	36.0
43	34.29787712416331	36.0	36.0	36.0	32.0	36.0
44	34.267045747151556	36.0	36.0	36.0	32.0	36.0
45	34.16942310180283	36.0	36.0	36.0	32.0	36.0
46	34.2302694134301	36.0	36.0	36.0	32.0	36.0
47	34.16692730020085	36.0	36.0	36.0	32.0	36.0
48	34.15041506675359	36.0	36.0	36.0	32.0	36.0
49	34.16399690003102	36.0	36.0	36.0	32.0	36.0
50	33.60111262257627	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	7.0
21	16.0
22	71.0
23	241.0
24	568.0
25	1386.0
26	2993.0
27	5846.0
28	9484.0
29	13865.0
30	20049.0
31	28729.0
32	42990.0
33	68024.0
34	148644.0
35	453222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.013818748272655	17.76952840380895	11.367427954071506	25.849224893846888
2	16.035111034627427	20.493202512740186	36.35408986023165	27.117596592400734
3	18.871924987596326	24.132339364555005	28.690360303214906	28.30537534463376
4	12.095280083704187	15.321609220523602	35.90726219231112	36.67584850346109
5	13.945212947017913	37.19359858918754	33.621725908983	15.239462554811547
6	34.9579720047831	35.1292995166655	16.398580142086278	13.514148336465126
7	30.24542258430396	30.707654587087397	20.642050300388	18.404872528220643
8	28.758869390569714	32.854395663057986	19.469764625937497	18.9169703204348
9	27.274074688150407	13.907405383746768	18.444438582806725	40.3740813452961
10	15.526347852191268	27.042205047623714	32.050639525609284	25.38080757457573
11	38.12861354264404	21.10063971401907	21.12852436201307	19.64222238132382
12	23.898525002606334	24.059678170968063	29.116094340546915	22.925702485878688
13	29.964189580436535	19.13075262172214	25.352169287446756	25.552888510394567
14	23.298502644645332	19.4864702934294	24.94269202411143	32.272335037813846
15	25.492094953506744	27.043461112848664	22.638817188498965	24.82562674514562
16	26.07754695485827	25.86125252312102	23.903926083073642	24.157274438947066
17	23.8185136477767	26.080310298353172	25.64998235228359	24.451193701586536
18	25.300293793656113	24.575920978424566	26.461400487604518	23.662384740314796
19	25.11037673164292	26.282913619138416	24.95763920028839	23.649070448930274
20	25.02132170719361	24.33689176611563	25.88612261457513	24.755663912115626
21	26.317078593257193	24.76935502306764	24.234020024191814	24.679546359483357
22	25.098820931573336	24.99506994399205	25.01692547890627	24.889183645528345
23	23.94864200508204	24.495030377937464	25.226813977996247	26.32951363898425
24	24.122732645260804	26.187955088131815	24.917947539179814	24.771364727427567
25	25.039409046432965	24.78744236230699	24.738204605488754	25.43494398577129
26	24.04912722307844	25.4988777057215	25.555777460411967	24.896217610788092
27	25.34739623959193	25.355811876599127	24.956131922018447	24.340659961790497
28	24.551930132627927	24.698764157425167	25.458809225045435	25.29049648490147
29	23.892998315616534	25.595217908475554	25.9790714412218	24.532712334686117
30	24.657565218046642	25.204832836559536	25.935611584438355	24.201990360955463
31	24.35950094016482	25.376913772378373	24.775760955714908	25.487824331741898
32	24.134916477942866	25.289366026199005	25.041418750792893	25.534298745065232
33	23.969115868248807	24.84157877350255	25.510182292746098	25.679123065502544
34	24.419415251395176	25.339859848242202	26.085585772297982	24.155139128064643
35	25.53304267984028	24.802891964573938	25.554395788664515	24.109669566921273
36	24.08065446022481	25.74205193327279	25.194658708237398	24.982634898264998
37	25.322274935092832	25.280573569624327	25.152580523201408	24.24457097208144
38	24.401830338245805	25.06201822048215	25.149440360139018	25.386711081133022
39	24.908903869560138	25.06729369442696	25.257210756440163	24.766591679572734
40	25.227190797563736	25.301549858881074	25.004490433179217	24.466768910375976
41	23.672810081681924	25.765791566024443	25.86816088185827	24.693237470435363
42	25.736022820193007	25.769559761699306	25.252563315107828	23.24185410299986
43	24.767973351320187	24.25424267431359	25.258592428187608	25.71919154617861
44	24.211662063187617	25.150445212318985	25.778729037841476	24.859163686651918
45	24.474430908248205	25.298786515386173	25.362343415768894	24.86443916059673
46	23.918646656584585	25.125230489416328	25.971446945983836	24.98467590801525
47	24.368042183694516	24.674270885538544	26.357147073933255	24.600539856833688
48	24.855269884454557	26.230661305780284	24.595515595933865	24.318553213831287
49	23.84790557404065	25.636040028286587	25.98296524341916	24.533089154253602
50	23.802465910346974	26.37049448837887	24.728438357265592	25.098601244008563
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	16.0
2	15.0
3	50.5
4	86.0
5	95.5
6	105.0
7	93.0
8	81.0
9	131.5
10	182.0
11	264.5
12	347.0
13	572.0
14	797.0
15	1082.5
16	1368.0
17	1636.0
18	1904.0
19	2260.0
20	2616.0
21	3154.0
22	3692.0
23	4203.5
24	4715.0
25	5270.0
26	5825.0
27	7055.5
28	8286.0
29	9540.5
30	10795.0
31	11791.0
32	12787.0
33	14614.5
34	16442.0
35	18956.5
36	21471.0
37	25282.5
38	29094.0
39	31800.0
40	34506.0
41	38606.0
42	42706.0
43	44359.5
44	46013.0
45	51591.5
46	57170.0
47	61205.5
48	65241.0
49	65617.5
50	65994.0
51	61332.5
52	56671.0
53	54473.0
54	52275.0
55	53370.5
56	54466.0
57	52399.5
58	50333.0
59	45491.5
60	40650.0
61	35599.0
62	30548.0
63	26752.5
64	22957.0
65	19914.5
66	16872.0
67	14508.0
68	12144.0
69	11204.0
70	10264.0
71	7928.0
72	5592.0
73	5137.0
74	4682.0
75	3301.5
76	1921.0
77	1764.0
78	1607.0
79	1404.0
80	1201.0
81	973.5
82	746.0
83	592.5
84	439.0
85	364.0
86	289.0
87	228.0
88	167.0
89	102.5
90	38.0
91	23.5
92	9.0
93	5.5
94	2.0
95	2.5
96	3.0
97	4.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014695963131973518
2	0.004270621764847005
3	2.512130449910003E-4
4	0.0
5	0.0
6	1.2560652249550016E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	6.280326124775007E-4
47	0.0
48	0.0
49	0.0
50	1.2560652249550016E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	796137.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.04584634985035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.61149975461275	48.40385728296476
2	12.489616005666866	14.998991272898085
3	3.3777425154993295	6.084582242850892
4	1.2782564407958112	3.070159593589274
5	0.6254782944168963	1.8778686780861709
6	0.34516107856584555	1.2435293453708032
7	0.2113545034253633	0.8883672026619799
8	0.15003169033294042	0.7207023860272054
9	0.11412219316960191	0.6167307308552872
>10	0.6325991972901007	7.353971585444599
>50	0.08196504568439425	3.4701989418644774
>100	0.07651338622184506	8.841594239673704
>500	0.005031017171848539	1.9905324550453087
>1k	6.288771464810674E-4	0.43891404266744605
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1293	0.1624092335866817	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1172	0.14721084436472617	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1022	0.12836986599040115	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	890	0.11178980502099511	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	880	0.11053373979604013	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	853	0.10714236368866163	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	803	0.10086203756388662	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	802	0.10073643104139111	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.2560652249550016E-4
11	0.0	0.0	0.0	1.2560652249550016E-4	1.2560652249550016E-4
12	0.0	0.0	0.0	1.2560652249550016E-4	2.512130449910003E-4
13	0.0	0.0	0.0	1.2560652249550016E-4	2.512130449910003E-4
14	0.0	0.0	0.0	1.2560652249550016E-4	2.512130449910003E-4
15	0.0	0.0	0.0	1.2560652249550016E-4	2.512130449910003E-4
16	1.2560652249550016E-4	0.0	0.0	1.2560652249550016E-4	2.512130449910003E-4
17	1.2560652249550016E-4	0.0	0.0	5.024260899820006E-4	2.512130449910003E-4
18	1.2560652249550016E-4	0.0	0.0	0.0010048521799640013	2.512130449910003E-4
19	1.2560652249550016E-4	0.0	0.0	0.0010048521799640013	2.512130449910003E-4
20	1.2560652249550016E-4	0.0	0.0	0.0012560652249550015	3.768195674865004E-4
21	1.2560652249550016E-4	0.0	0.0	0.0018840978374325022	3.768195674865004E-4
22	1.2560652249550016E-4	0.0	0.0	0.003265769584883004	3.768195674865004E-4
23	1.2560652249550016E-4	0.0	0.0	0.006531539169766008	3.768195674865004E-4
24	1.2560652249550016E-4	0.0	0.0	0.009922915277144511	3.768195674865004E-4
25	1.2560652249550016E-4	0.0	0.0	0.012686258772045515	3.768195674865004E-4
26	1.2560652249550016E-4	0.0	0.0	0.015575208789442019	3.768195674865004E-4
27	1.2560652249550016E-4	0.0	0.0	0.01909219141931602	3.768195674865004E-4
28	1.2560652249550016E-4	0.0	0.0	0.02662858276904603	3.768195674865004E-4
29	1.2560652249550016E-4	0.0	0.0	0.03931484154109154	3.768195674865004E-4
30	1.2560652249550016E-4	0.0	0.0	0.06493857213017358	3.768195674865004E-4
31	1.2560652249550016E-4	0.0	0.0	0.11065934631853563	3.768195674865004E-4
32	1.2560652249550016E-4	0.0	0.0	0.1681871336214747	3.768195674865004E-4
33	1.2560652249550016E-4	0.0	0.0	0.21654564478224225	3.768195674865004E-4
34	1.2560652249550016E-4	0.0	0.0	0.27960011907498333	3.768195674865004E-4
35	1.2560652249550016E-4	0.0	0.0	0.3594858673821214	3.768195674865004E-4
36	1.2560652249550016E-4	0.0	0.0	0.46172957669345854	3.768195674865004E-4
37	1.2560652249550016E-4	0.0	0.0	0.6109501254181127	3.768195674865004E-4
38	1.2560652249550016E-4	0.0	0.0	0.7839103068944164	5.024260899820006E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGAAAC	35	3.217045E-4	31.4283	28
CGCAATA	225	0.0	21.51093	36
TCTAGCG	245	0.0	20.652884	28
CCGGTCC	290	0.0	19.723969	9
CGGTCCA	260	0.0	19.461372	10
CTAGCGG	260	0.0	19.461372	29
TAGCGGC	265	0.0	19.094177	30
ATATATC	140	4.0727173E-9	18.858166	3
ATACGAA	280	0.0	18.856981	40
CGTCTAT	60	0.007399569	18.337784	1
CCCTATA	60	0.007407617	18.334328	2
AGTAAGG	60	0.007407617	18.334328	2
GATTGCG	60	0.0074103014	18.333176	12
CGAATGC	265	0.0	18.263994	43
GTCTAGG	135	5.5031705E-8	17.930279	1
GCGCAAT	270	0.0	17.925772	35
CAATACG	285	0.0	17.754232	38
CCCCTAT	75	0.0012882473	17.604273	1
GCATTAG	100	2.4761164E-5	17.604273	1
ATATACT	125	4.8477705E-7	17.59985	4
>>END_MODULE