FastQCFastQC Report
Thu 2 Feb 2017
SRR4062715_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062715_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355750
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG168201.2406417112299466No Hit
TCCATGTACTCTGCGTTGATACCAC138901.0245251705697953No Hit
GTATCAACGCAGAGTACTTTTTTTT115170.8494929006085193No Hit
GTACTTTTTTTTTTTTTTTTTTTTT89870.6628803245436106No Hit
CATGTACTCTGCGTTGATACCACTG77060.5683938779273465No Hit
GAGTACATGGAAGCAGTGGTATCAA72510.5348331182002581No Hit
TATCAACGCAGAGTACTTTTTTTTT70720.5216300940438872No Hit
GAGTACTTTTTTTTTTTTTTTTTTT69030.5091646690023972No Hit
CCCATGTACTCTGCGTTGATACCAC66620.4913885303337636No Hit
GTACATGGGAAGCAGTGGTATCAAC64740.47752166697399967No Hit
GTCCTAAAGTGTGTATTTCTCATTT49960.36850451779457866No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49400.3643739627512447No Hit
GTCCTACAGTGGACATTTCTAAATT45160.33309976028028765No Hit
GCTTCCATGTACTCTGCGTTGATAC43370.31989673612391667No Hit
CTTTAGGACGTGAAATATGGCGAGG41990.30971786833855797No Hit
CATGGAAGCAGTGGTATCAACGCAG41200.30389083533099764No Hit
GCGTTGATACCACTGCTTCCATGTA40620.29961276046468743No Hit
CTGTAGGACGTGGAATATGGCAAGA39960.2947446063064724No Hit
GGTATCAACGCAGAGTACTTTTTTT38540.2842706988751614No Hit
GTACATGGGGTGGTATCAACGCAAA37180.2742393509127789No Hit
GTCCTACAGTGTGCATTTCTCATTT37170.2741655910012908No Hit
GTATCAACGCAGAGTACATGGAAGC31200.23013092384289138No Hit
ACTCTGCGTTGATACCACTGCTTCC29460.2172966992439609No Hit
GTATCAACGCAGAGTACATGGGAAG28450.20984694818366217No Hit
CAGTGGTATCAACGCAGAGTACATG27560.2032823160612207No Hit
GAGTACATGGGAAGCAGTGGTATCA26470.19524248570901714No Hit
GTACATGGGTGGTATCAACGCAAAA25500.18808777429467086No Hit
GCAGAGTACTTTTTTTTTTTTTTTT24380.17982666420800295No Hit
GTATTAGAGGCACTGCCTGCCCAGT23820.17569610916466902No Hit
ACGCAGAGTACATGGAAGCAGTGGT23800.1755485893416928No Hit
CTGTAGGACCTGGAATATGGCGAGA23580.17392587128895445No Hit
CCATAGGGTCTTCTCGTCTTATTAT23360.17230315323621612No Hit
CTGAAGGACCTGGAATATGGCGAGA21330.15732989120413057No Hit
GCGTTGATACCACTGCTTCCCATGT20870.15393693527567767No Hit
TATCAACGCAGAGTACATGGAAGCA20560.15165037801954637No Hit
CTCTAATACTTGTAATGCTAGAGGT20090.14818366217960538No Hit
GTCCTTCAGTGTGCATTTCTCATTT19920.14692974368430758No Hit
GCTTCCCATGTACTCTGCGTTGATA19760.14574958510049787No Hit
CCATTGGGATGTCCTGATCCAACAT18690.1378572745712705No Hit
ATTTAGAAATGTCCACTGTAGGACG18350.13534943758067491No Hit
GTACATGGGAGTGGTATCAACGCAA18220.13439055873132952No Hit
TTGTAGAACAGTGTATATCAATGAG18040.1330628803245436No Hit
GATATACACTGTTCTACAAATCCCG17230.127088327494007No Hit
GTGTATATCAATGAGTTACAATGAA16990.12531808961829247No Hit
TATCAACGCAGAGTACATGGGAAGC16940.12494929006085193No Hit
GTATCAACGCAGAGTACATGGGGTG16780.12376913147704224No Hit
GATATATTTTGATCAACGGACCAAG16750.1235478517425779No Hit
GGTATCAACGCAGAGTACATGGGAA16500.12170385395537525No Hit
ATCTATAACTTTATAGATGCAACAC15800.11654066015120781No Hit
ATACCACTGCTTCCATGTACTCTGC15760.1162456205052554No Hit
GTTATAGATTAACCCAATTTTAAGT15760.1162456205052554No Hit
CTTCTACACCATTGGGATGTCCTGA15630.11528674165591No Hit
GTACTCTGCGTTGATACCACTGCTT15510.11440162271805274No Hit
GAATATGGCAAGAAAACTGAAAATC15250.11248386501936199No Hit
TTTCTAAATTTTCCACCTTTTTCAG14950.11027106767471878No Hit
AAGCAGTGGTATCAACGCAGAGTAC14830.1093859487368615No Hit
GTCAGGATACCGCGGCCGTTAAACT14820.10931218882537341No Hit
GGTATCAACGCAGAGTACATGGAAG14770.10894338926793287No Hit
ATCAACGCAGAGTACTTTTTTTTTT14320.10562419325096808No Hit
CTGTTAGTATGAGTAACAAGAATTC14270.10525539369352756No Hit
GGAATATGGCGAGAAAACTGAAAAT14140.10429651484418219No Hit
CTATAGAACTAGTACCGCAAGGGAA13710.10112483865019362No Hit
ATCCTGACCGTGCAAAGGTAGCATA13580.10016595980084823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCT350.00216862416.2872667
TCTAAGC350.00217559316.2794463
AACTTGC1250.015.2014497
TGCACCG508.7192646E-515.198645
CACCTTT5350.014.02885614
TAGGACC10050.013.798744
CCACCTT5350.013.49611513
TTGTAGA4000.013.2899711
AAATGTC6950.013.1235537
AAGGCGA801.992581E-613.06615817
TAGAAAT7200.013.0603674
AAACTTG1550.012.8721946
TTTAGAA7200.012.7917952
GGCGAGG12250.012.721930519
AATGTCC7250.012.7115568
GGACCTA1051.9897925E-812.6678736
CTAAGGT751.48073395E-512.66459754
GTCTAGA905.4528755E-712.6571161
TCCTATA1900.012.4933422
GAAATGT7200.012.403966
Produced by FastQC (version 0.11.5)