##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062715_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1355750
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.876101051078738	32.0	32.0	32.0	32.0	32.0
2	30.839456389452334	32.0	32.0	32.0	32.0	32.0
3	30.865577724506732	32.0	32.0	32.0	32.0	32.0
4	30.793823345011987	32.0	32.0	32.0	32.0	32.0
5	30.863006453992256	32.0	32.0	32.0	32.0	32.0
6	34.41058454729854	36.0	36.0	36.0	32.0	36.0
7	34.16195242485709	36.0	36.0	36.0	32.0	36.0
8	34.13746413424304	36.0	36.0	36.0	32.0	36.0
9	34.13902120597455	36.0	36.0	36.0	32.0	36.0
10	34.07677964226443	36.0	36.0	36.0	32.0	36.0
11	34.33781596902084	36.0	36.0	36.0	32.0	36.0
12	34.170810252627696	36.0	36.0	36.0	32.0	36.0
13	34.24376396828324	36.0	36.0	36.0	32.0	36.0
14	34.19535238797713	36.0	36.0	36.0	32.0	36.0
15	34.225859487368616	36.0	36.0	36.0	32.0	36.0
16	34.19438170754195	36.0	36.0	36.0	32.0	36.0
17	34.14873907431311	36.0	36.0	36.0	32.0	36.0
18	34.2248275862069	36.0	36.0	36.0	32.0	36.0
19	34.13337119675457	36.0	36.0	36.0	32.0	36.0
20	34.04797934722478	36.0	36.0	36.0	32.0	36.0
21	33.94756334132399	36.0	36.0	36.0	32.0	36.0
22	33.9176854139775	36.0	36.0	36.0	32.0	36.0
23	33.919281578462105	36.0	36.0	36.0	32.0	36.0
24	33.909197860962564	36.0	36.0	36.0	32.0	36.0
25	33.368623271252076	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	63.0
5	130.0
6	215.0
7	56.0
8	197.0
9	268.0
10	176.0
11	54.0
12	97.0
13	88.0
14	517.0
15	726.0
16	1106.0
17	1604.0
18	2309.0
19	3181.0
20	4400.0
21	5506.0
22	6840.0
23	8361.0
24	9787.0
25	11687.0
26	14486.0
27	17831.0
28	23255.0
29	30701.0
30	40100.0
31	55552.0
32	80805.0
33	119485.0
34	284271.0
35	631885.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.713494940374765	20.548540387744772	12.451959747247415	23.28600492463305
2	12.31739151922142	22.69025397193039	43.17618707959941	21.816167429248782
3	18.542281364333764	29.330004130768323	30.38784964003305	21.739864864864867
4	10.52229331314951	18.39361553800291	40.07263520616492	31.011455942682666
5	9.897473925763595	41.29886037048105	36.54458722094941	12.259078482805947
6	27.25494985392664	39.52525973312002	20.391900178652996	12.827890234300343
7	24.47664557158231	34.43266918508611	23.8361905436745	17.254494699657073
8	24.738082002056807	37.29419937734722	22.164785198080214	15.802933422515755
9	26.91083766610603	15.751111557391212	22.05535120192911	35.28269957457365
10	14.970825791462566	28.836273647503486	35.54637853581733	20.64652202521662
11	31.492739687786635	23.812066401097727	26.64618034169477	18.049013569420875
12	23.39035379274688	26.955388137971788	32.71482384223355	16.939434227047787
13	28.94978612279624	23.428933095429407	27.843843054308266	19.777437727466086
14	20.68972902711206	24.743982688458726	28.411828568474384	26.154459715954832
15	22.28024041241407	32.48506653138114	27.337127530972776	17.897565525232004
16	19.985496618954677	28.919254419875834	32.029505961967764	19.065742999201724
17	18.517536539072253	29.878067694216526	31.865985244049245	19.73841052266198
18	19.54543305946757	26.399979910602177	36.56735709200058	17.48722993792967
19	21.396890628381417	29.485555507136013	29.67508108016282	19.44247278431975
20	21.137983825168252	28.046369524968632	33.366147765381484	17.449498884481628
21	21.002038495058866	27.613852903378287	28.197039750653648	23.1870688509092
22	20.526475981093967	31.667013878115487	28.50295720729705	19.303552933493496
23	18.64443211708594	30.99048684120138	30.46781340647019	19.897267635242493
24	20.295937488689805	30.994994338439447	30.614380880839846	18.0946872920309
25	20.79711707772486	29.720349448705374	30.400604474172464	19.0819289993973
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	568.0
1	568.0
2	1559.5
3	2551.0
4	2551.0
5	2551.0
6	7991.0
7	13431.0
8	13431.0
9	13431.0
10	13125.0
11	12819.0
12	12819.0
13	12819.0
14	11244.5
15	9670.0
16	9670.0
17	9670.0
18	16530.0
19	23390.0
20	23390.0
21	23390.0
22	37118.0
23	50846.0
24	50846.0
25	50846.0
26	75837.5
27	100829.0
28	100829.0
29	100829.0
30	124957.0
31	149085.0
32	149085.0
33	149085.0
34	151824.5
35	154564.0
36	154564.0
37	154564.0
38	149472.5
39	144381.0
40	144381.0
41	144381.0
42	147256.5
43	150132.0
44	150132.0
45	150132.0
46	193251.0
47	236370.0
48	236370.0
49	236370.0
50	188655.5
51	140941.0
52	140941.0
53	140941.0
54	102519.0
55	64097.0
56	64097.0
57	64097.0
58	50563.5
59	37030.0
60	37030.0
61	37030.0
62	31276.5
63	25523.0
64	25523.0
65	25523.0
66	21438.5
67	17354.0
68	17354.0
69	17354.0
70	13858.5
71	10363.0
72	10363.0
73	10363.0
74	8289.0
75	6215.0
76	6215.0
77	6215.0
78	4613.5
79	3012.0
80	3012.0
81	3012.0
82	2189.5
83	1367.0
84	1367.0
85	1367.0
86	928.0
87	489.0
88	489.0
89	489.0
90	320.0
91	151.0
92	151.0
93	151.0
94	99.5
95	48.0
96	48.0
97	48.0
98	286.0
99	524.0
100	524.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008556149732620321
2	0.003614235662917204
3	0.005163193804167436
4	0.015415821501014199
5	0.035773557071731514
6	0.044919786096256686
7	0.068744237506915
8	0.0902083717499539
9	0.09913332104001474
10	0.12118753457495851
11	0.12362161165406602
12	0.13645583625299648
13	0.12553936935275678
14	0.1283422459893048
15	0.1163193804167435
16	0.11676193988567214
17	0.1268670477595427
18	0.1329153605015674
19	0.13593951687257974
20	0.12325281209662549
21	0.13350543979347226
22	0.1554858934169279
23	0.14316798819841417
24	0.13888991333210401
25	0.13616079660704408
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1355750.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.542531660886592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.93231543047413	18.710279485411686
2	15.799823053209103	8.703342533588351
3	6.249753683778993	5.164021161246765
4	3.085813128478507	3.399644231627951
5	1.7070777862608295	2.3508621987842546
6	1.0609104935718046	1.7532096515140947
7	0.6824861666796733	1.3158177797724417
8	0.5122063077698535	1.128596675892561
9	0.36005358355474065	0.8925108502204909
>10	2.071983786896368	10.659945593576523
>50	0.22327317494172153	4.33639670626589
>100	0.24804921632258126	14.55625662101573
>500	0.040398895159571145	7.935615559858998
>1k	0.02316203322482079	12.173917755879748
>5k	0.0018852817741133203	3.787218542037233
>10k+	8.079779031914229E-4	3.13236465330734
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	16820	1.2406417112299466	No Hit
TCCATGTACTCTGCGTTGATACCAC	13890	1.0245251705697953	No Hit
GTATCAACGCAGAGTACTTTTTTTT	11517	0.8494929006085193	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	8987	0.6628803245436106	No Hit
CATGTACTCTGCGTTGATACCACTG	7706	0.5683938779273465	No Hit
GAGTACATGGAAGCAGTGGTATCAA	7251	0.5348331182002581	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7072	0.5216300940438872	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	6903	0.5091646690023972	No Hit
CCCATGTACTCTGCGTTGATACCAC	6662	0.4913885303337636	No Hit
GTACATGGGAAGCAGTGGTATCAAC	6474	0.47752166697399967	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4996	0.36850451779457866	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4940	0.3643739627512447	No Hit
GTCCTACAGTGGACATTTCTAAATT	4516	0.33309976028028765	No Hit
GCTTCCATGTACTCTGCGTTGATAC	4337	0.31989673612391667	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4199	0.30971786833855797	No Hit
CATGGAAGCAGTGGTATCAACGCAG	4120	0.30389083533099764	No Hit
GCGTTGATACCACTGCTTCCATGTA	4062	0.29961276046468743	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3996	0.2947446063064724	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3854	0.2842706988751614	No Hit
GTACATGGGGTGGTATCAACGCAAA	3718	0.2742393509127789	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3717	0.2741655910012908	No Hit
GTATCAACGCAGAGTACATGGAAGC	3120	0.23013092384289138	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2946	0.2172966992439609	No Hit
GTATCAACGCAGAGTACATGGGAAG	2845	0.20984694818366217	No Hit
CAGTGGTATCAACGCAGAGTACATG	2756	0.2032823160612207	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2647	0.19524248570901714	No Hit
GTACATGGGTGGTATCAACGCAAAA	2550	0.18808777429467086	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	2438	0.17982666420800295	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	2382	0.17569610916466902	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2380	0.1755485893416928	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2358	0.17392587128895445	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	2336	0.17230315323621612	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2133	0.15732989120413057	No Hit
GCGTTGATACCACTGCTTCCCATGT	2087	0.15393693527567767	No Hit
TATCAACGCAGAGTACATGGAAGCA	2056	0.15165037801954637	No Hit
CTCTAATACTTGTAATGCTAGAGGT	2009	0.14818366217960538	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1992	0.14692974368430758	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1976	0.14574958510049787	No Hit
CCATTGGGATGTCCTGATCCAACAT	1869	0.1378572745712705	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1835	0.13534943758067491	No Hit
GTACATGGGAGTGGTATCAACGCAA	1822	0.13439055873132952	No Hit
TTGTAGAACAGTGTATATCAATGAG	1804	0.1330628803245436	No Hit
GATATACACTGTTCTACAAATCCCG	1723	0.127088327494007	No Hit
GTGTATATCAATGAGTTACAATGAA	1699	0.12531808961829247	No Hit
TATCAACGCAGAGTACATGGGAAGC	1694	0.12494929006085193	No Hit
GTATCAACGCAGAGTACATGGGGTG	1678	0.12376913147704224	No Hit
GATATATTTTGATCAACGGACCAAG	1675	0.1235478517425779	No Hit
GGTATCAACGCAGAGTACATGGGAA	1650	0.12170385395537525	No Hit
ATCTATAACTTTATAGATGCAACAC	1580	0.11654066015120781	No Hit
ATACCACTGCTTCCATGTACTCTGC	1576	0.1162456205052554	No Hit
GTTATAGATTAACCCAATTTTAAGT	1576	0.1162456205052554	No Hit
CTTCTACACCATTGGGATGTCCTGA	1563	0.11528674165591	No Hit
GTACTCTGCGTTGATACCACTGCTT	1551	0.11440162271805274	No Hit
GAATATGGCAAGAAAACTGAAAATC	1525	0.11248386501936199	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1495	0.11027106767471878	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1483	0.1093859487368615	No Hit
GTCAGGATACCGCGGCCGTTAAACT	1482	0.10931218882537341	No Hit
GGTATCAACGCAGAGTACATGGAAG	1477	0.10894338926793287	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1432	0.10562419325096808	No Hit
CTGTTAGTATGAGTAACAAGAATTC	1427	0.10525539369352756	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1414	0.10429651484418219	No Hit
CTATAGAACTAGTACCGCAAGGGAA	1371	0.10112483865019362	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	1358	0.10016595980084823	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.2127973446431863E-4	0.0
8	0.0	0.0	0.0	4.4255946892863727E-4	0.0
9	0.0	0.0	0.0	5.900792919048498E-4	0.0
10	0.0	0.0	0.0	5.900792919048498E-4	0.0
11	0.0	0.0	0.0	5.900792919048498E-4	0.0
12	0.0	0.0	0.0	5.900792919048498E-4	0.0
13	0.0	0.0	0.0	5.900792919048498E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCT	35	0.002168624	16.287266	7
TCTAAGC	35	0.002175593	16.279446	3
AACTTGC	125	0.0	15.201449	7
TGCACCG	50	8.7192646E-5	15.19864	5
CACCTTT	535	0.0	14.028856	14
TAGGACC	1005	0.0	13.79874	4
CCACCTT	535	0.0	13.496115	13
TTGTAGA	400	0.0	13.289971	1
AAATGTC	695	0.0	13.123553	7
AAGGCGA	80	1.992581E-6	13.066158	17
TAGAAAT	720	0.0	13.060367	4
AAACTTG	155	0.0	12.872194	6
TTTAGAA	720	0.0	12.791795	2
GGCGAGG	1225	0.0	12.7219305	19
AATGTCC	725	0.0	12.711556	8
GGACCTA	105	1.9897925E-8	12.667873	6
CTAAGGT	75	1.48073395E-5	12.6645975	4
GTCTAGA	90	5.4528755E-7	12.657116	1
TCCTATA	190	0.0	12.493342	2
GAAATGT	720	0.0	12.40396	6
>>END_MODULE