FastQCFastQC Report
Thu 2 Feb 2017
SRR4062715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355750
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA77180.5692789968652037No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC67060.49463396643924024No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA36780.2712889544532547No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC29540.21788677853586574No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA28590.21087958694449566No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24250.17886778535865758No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT23570.17385211137746637No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG21830.16101788677853587No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21800.16079660704407156No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA20900.15415821501014199No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19990.14744606306472433No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA18920.13955375253549696No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT18840.1389636732435921No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG18820.13881615342061587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18750.13829983404019916No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA18470.13623455651853217No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC18190.1341692789968652No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA17390.1282684860778167No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA17240.12716208740549512No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG17170.12664576802507838No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC17000.12539184952978058No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA16910.12472801032638761No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC15340.11314770422275493No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA14660.1081320302415637No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC14130.10422275493269409No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA13850.1021574774110271No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA13810.1018624377650747No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA13680.1009035589157293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC401.8202878E-532.9998634
ATACATG3050.026.6923481
TAGGACC12400.024.4837684
ATAGGAC2250.024.4443423
ACGGTAA651.8136605E-523.6922138
ACTGTTC9100.020.5493648
TATAGGA2950.020.1362512
AGTACGT1101.2659802E-719.9999145
AGGACCT20550.019.9123255
TTGCGAG1001.123688E-619.79991718
TGTAGGA35350.019.5423662
AACTTGC2150.019.441787
TTAGGAC20550.019.3770483
CTGTAGG33450.019.2733151
TGTCCAC14400.019.2499210
GTCCTAA17700.019.1439881
TCCTACA32100.019.0535832
GTAGTAC1851.8189894E-1219.0269493
TAGGACA8450.019.0058384
GGACCTG20150.018.9974386
Produced by FastQC (version 0.11.5)