##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062715_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1355750
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.962086667896	32.0	32.0	32.0	32.0	32.0
2	31.44087995574405	32.0	32.0	32.0	32.0	32.0
3	31.529845472985432	32.0	32.0	32.0	32.0	32.0
4	31.63298543241748	32.0	32.0	32.0	32.0	32.0
5	31.596089987092014	32.0	32.0	32.0	32.0	32.0
6	35.15717278259266	36.0	36.0	36.0	36.0	36.0
7	35.18170680435183	36.0	36.0	36.0	36.0	36.0
8	35.12512557624931	36.0	36.0	36.0	36.0	36.0
9	35.179239535312554	36.0	36.0	36.0	36.0	36.0
10	35.129760649087224	36.0	36.0	36.0	36.0	36.0
11	35.204545823345015	36.0	36.0	36.0	36.0	36.0
12	35.121739996312	36.0	36.0	36.0	36.0	36.0
13	35.149672137193434	36.0	36.0	36.0	36.0	36.0
14	35.10434298358842	36.0	36.0	36.0	36.0	36.0
15	35.10449861700166	36.0	36.0	36.0	36.0	36.0
16	35.1134869998156	36.0	36.0	36.0	36.0	36.0
17	35.126337451595056	36.0	36.0	36.0	36.0	36.0
18	35.11441784989858	36.0	36.0	36.0	36.0	36.0
19	35.047329522404574	36.0	36.0	36.0	36.0	36.0
20	34.980956666052	36.0	36.0	36.0	36.0	36.0
21	34.92650046099945	36.0	36.0	36.0	36.0	36.0
22	34.92217960538447	36.0	36.0	36.0	36.0	36.0
23	34.9019826664208	36.0	36.0	36.0	36.0	36.0
24	34.89040457311451	36.0	36.0	36.0	36.0	36.0
25	34.86549437580675	36.0	36.0	36.0	36.0	36.0
26	34.8178735017518	36.0	36.0	36.0	32.0	36.0
27	34.77828729485525	36.0	36.0	36.0	32.0	36.0
28	34.7363717499539	36.0	36.0	36.0	32.0	36.0
29	34.678973999631204	36.0	36.0	36.0	32.0	36.0
30	34.64146487184215	36.0	36.0	36.0	32.0	36.0
31	34.59506472432233	36.0	36.0	36.0	32.0	36.0
32	34.535457127051444	36.0	36.0	36.0	32.0	36.0
33	34.47208998709201	36.0	36.0	36.0	32.0	36.0
34	34.40306988751613	36.0	36.0	36.0	32.0	36.0
35	34.30700792919048	36.0	36.0	36.0	32.0	36.0
36	34.22625189009773	36.0	36.0	36.0	32.0	36.0
37	34.142808777429465	36.0	36.0	36.0	32.0	36.0
38	34.05672579752904	36.0	36.0	36.0	32.0	36.0
39	33.87146524064171	36.0	36.0	36.0	32.0	36.0
40	33.76992660888807	36.0	36.0	36.0	32.0	36.0
41	33.74617739258713	36.0	36.0	36.0	27.0	36.0
42	33.61157514290983	36.0	36.0	36.0	21.0	36.0
43	33.59518274018071	36.0	36.0	36.0	21.0	36.0
44	33.54892568688918	36.0	36.0	36.0	21.0	36.0
45	33.31691757329891	36.0	36.0	36.0	14.0	36.0
46	33.3075589157293	36.0	36.0	36.0	14.0	36.0
47	33.190270330075606	36.0	36.0	36.0	14.0	36.0
48	33.11422828692606	36.0	36.0	36.0	14.0	36.0
49	33.098105845472986	36.0	36.0	36.0	14.0	36.0
50	32.53880803983035	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	15.0
21	44.0
22	128.0
23	467.0
24	1315.0
25	3395.0
26	7454.0
27	15167.0
28	25472.0
29	36379.0
30	47175.0
31	62009.0
32	85336.0
33	110392.0
34	205878.0
35	755118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.847383455007936	21.404721997627494	13.062585206650102	23.68530934071447
2	11.940075016873264	23.153880481966816	42.61880437709072	22.287240124069204
3	18.219878604952726	29.4384833658986	30.70642653195047	21.635211497198206
4	10.422283180736256	18.786884592944563	40.03757332662609	30.753258899693087
5	9.834999078001108	41.31381154342615	36.55024893970128	12.300940438871473
6	27.62892164166444	39.60262468043424	19.914497258336	12.853956419565316
7	24.343721187534577	34.27807486631016	24.054361054766733	17.32384289138853
8	24.780969942836066	37.48921261294487	22.047796422644293	15.682021021574775
9	26.839166513000183	15.402249677300386	22.077374147151023	35.681209662548405
10	15.030647243223308	28.768873317352018	35.64189562972525	20.558583809699428
11	31.53258344089987	23.50610363267564	26.843665867600958	18.11764705882353
12	23.13383735939517	27.005863912963306	32.89618292458049	16.964115803061038
13	29.153826295408447	23.120486815415823	27.9987460815047	19.72694080767103
14	20.60483127420247	24.606675271989673	28.447427623086853	26.341065830721007
15	22.39218144938226	32.51801585838097	27.10853770975475	17.98126498248202
16	20.147814862622166	28.61442006269592	31.938853033376358	19.29891204130555
17	18.568467637838832	29.754748294302047	31.87777982666421	19.79900424119491
18	19.780047943942467	25.912963304444038	36.587718974737236	17.719269776876267
19	21.62795500645399	29.25450857458971	29.85402913516504	19.26350728379126
20	21.278333026000368	27.92019177576987	33.34331550802139	17.458159690208372
21	21.033007560390928	27.485082057901533	28.435552277337266	23.046358104370274
22	20.642375069149917	31.18620689655172	28.74822054213535	19.42319749216301
23	18.717536418956296	30.383772819472615	30.70875898948921	20.189931772081874
24	20.445731145122625	30.780896182924582	30.552461737045917	18.22091093490688
25	21.119527936566477	28.99760280287664	30.42146413424304	19.46140512631385
26	16.92738336713996	29.553678775585468	33.16843075788309	20.35050709939148
27	19.81073206712152	31.022607412871107	30.999594320486818	18.16706619952056
28	18.301309238428914	29.251041858749772	30.98617001659598	21.461478886225336
29	19.108168910197307	29.340438871473356	31.172340033191958	20.37905218513738
30	20.00855614973262	28.426258528489768	31.86103632675641	19.704148995021207
31	20.031126682647983	27.968356997971604	30.812170385395536	21.188345933984877
32	17.438318274018073	30.96942651668818	31.707468191038167	19.88478701825558
33	17.88781117462659	30.895371565554118	32.23610547667343	18.98071178314586
34	17.85904480914623	30.7832565000922	33.59210768947077	17.765591001290797
35	19.470846394984324	31.123142172229397	31.19808224230131	18.207929190484972
36	18.686040936750874	30.064761202286554	31.967029319564816	19.282168541397752
37	19.660630647243224	30.3510234187719	29.395242485709016	20.593103448275862
38	19.364410842706988	32.02979900424119	29.008519269776876	19.597270883274938
39	19.699944680066384	29.62802876636548	29.95087589894892	20.721150654619215
40	21.25222201733358	29.85041489950212	30.46232712520745	18.43503595795685
41	19.89835884196939	28.51167250599299	31.871583994099208	19.718384657938408
42	20.166476120228655	31.824746450304257	30.43621611654066	17.572561312926425
43	17.996238244514107	30.978277706066752	30.341877189747375	20.68360685967177
44	17.795463765443483	30.36371012354785	32.01276046468744	19.828065646321225
45	18.932472801032638	30.586833855799373	30.7088327494007	19.771860593767286
46	19.23737927310868	28.83873527932223	30.878276609335376	21.045608838233708
47	21.630094043887148	28.573925871288953	30.353973815231424	19.442006269592476
48	18.124875530149364	32.30772635072838	28.292900608519272	21.274497510602988
49	18.812022865572562	29.09710492347409	31.68452885856537	20.406343352387974
50	16.886459071701328	33.46924836381956	27.85980295762711	21.784489606852006
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	66.0
1	84.0
2	102.0
3	291.5
4	481.0
5	520.0
6	559.0
7	556.0
8	553.0
9	824.0
10	1095.0
11	1571.0
12	2047.0
13	3677.0
14	5307.0
15	7806.5
16	10306.0
17	13581.0
18	16856.0
19	18785.5
20	20715.0
21	23188.5
22	25662.0
23	25405.5
24	25149.0
25	27334.0
26	29519.0
27	37207.5
28	44896.0
29	51724.5
30	58553.0
31	63393.5
32	68234.0
33	80714.0
34	93194.0
35	105997.5
36	118801.0
37	121384.5
38	123968.0
39	113719.5
40	103471.0
41	98328.0
42	93185.0
43	84487.5
44	75790.0
45	80093.5
46	84397.0
47	88046.0
48	91695.0
49	79491.0
50	67287.0
51	55572.5
52	43858.0
53	35740.0
54	27622.0
55	25451.0
56	23280.0
57	22881.0
58	22482.0
59	19727.0
60	16972.0
61	15628.0
62	14284.0
63	12995.0
64	11706.0
65	10666.5
66	9627.0
67	8366.5
68	7106.0
69	6408.5
70	5711.0
71	4849.5
72	3988.0
73	3413.5
74	2839.0
75	2316.0
76	1793.0
77	1468.0
78	1143.0
79	913.5
80	684.0
81	530.0
82	376.0
83	301.0
84	226.0
85	164.5
86	103.0
87	66.0
88	29.0
89	21.0
90	13.0
91	9.0
92	5.0
93	5.5
94	6.0
95	4.0
96	2.0
97	2.0
98	2.0
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01637470035035958
2	0.004056795131845842
3	8.113590263691685E-4
4	7.375991148810622E-5
5	0.0
6	2.950396459524249E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.950396459524249E-4
47	0.0
48	0.0
49	0.0
50	7.375991148810622E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1355750.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.79870944959432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.57042103510533	28.156386803757794
2	14.007492380896574	10.869452540076246
3	5.364660412544198	6.244257019261294
4	2.516827394882556	3.9059861931531077
5	1.4317080481299782	2.7774212288020417
6	0.8807196501566142	2.05024714877849
7	0.5688067317874963	1.5448276983717477
8	0.4040069874982722	1.253995977884108
9	0.2960391143534356	1.033734202516276
>10	1.6159112672097327	11.527678065222478
>50	0.15473528764802677	4.218665411842341
>100	0.1545073372344654	12.741051465322183
>500	0.023416016918642424	6.376110584038436
>1k	0.010364466504972875	6.226057293682715
>5k	3.8386912981381026E-4	1.0741283672907709
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	7718	0.5692789968652037	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	6706	0.49463396643924024	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3678	0.2712889544532547	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2954	0.21788677853586574	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2859	0.21087958694449566	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2425	0.17886778535865758	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	2357	0.17385211137746637	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	2183	0.16101788677853587	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2180	0.16079660704407156	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2090	0.15415821501014199	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1999	0.14744606306472433	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1892	0.13955375253549696	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	1884	0.1389636732435921	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1882	0.13881615342061587	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1875	0.13829983404019916	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1847	0.13623455651853217	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1819	0.1341692789968652	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1739	0.1282684860778167	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	1724	0.12716208740549512	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1717	0.12664576802507838	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	1700	0.12539184952978058	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1691	0.12472801032638761	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	1534	0.11314770422275493	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	1466	0.1081320302415637	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	1413	0.10422275493269409	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	1385	0.1021574774110271	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	1381	0.1018624377650747	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	1368	0.1009035589157293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.375991148810622E-5	0.0
5	0.0	0.0	0.0	7.375991148810622E-5	0.0
6	0.0	0.0	0.0	7.375991148810622E-5	0.0
7	0.0	0.0	0.0	7.375991148810622E-5	0.0
8	0.0	0.0	0.0	1.4751982297621244E-4	0.0
9	0.0	0.0	0.0	2.2127973446431863E-4	0.0
10	7.375991148810622E-5	0.0	0.0	2.2127973446431863E-4	0.0
11	7.375991148810622E-5	0.0	0.0	2.2127973446431863E-4	0.0
12	7.375991148810622E-5	0.0	0.0	2.2127973446431863E-4	0.0
13	7.375991148810622E-5	0.0	0.0	2.2127973446431863E-4	0.0
14	7.375991148810622E-5	0.0	0.0	2.2127973446431863E-4	0.0
15	7.375991148810622E-5	0.0	0.0	2.2127973446431863E-4	0.0
16	1.4751982297621244E-4	0.0	0.0	2.950396459524249E-4	0.0
17	1.4751982297621244E-4	0.0	0.0	5.163193804167435E-4	0.0
18	1.4751982297621244E-4	0.0	0.0	6.63839203392956E-4	0.0
19	1.4751982297621244E-4	0.0	0.0	9.588788493453807E-4	0.0
20	1.4751982297621244E-4	0.0	0.0	0.001327678406785912	0.0
21	2.2127973446431863E-4	0.0	0.0	0.0021390374331550803	0.0
22	2.2127973446431863E-4	0.0	0.0	0.003540475751429098	0.0
23	2.2127973446431863E-4	0.0	0.0	0.006195832565000922	0.0
24	2.2127973446431863E-4	0.0	0.0	0.011063986723215933	0.0
25	2.2127973446431863E-4	0.0	0.0	0.01401438318274018	0.0
26	2.2127973446431863E-4	0.0	0.0	0.019546376544348147	0.0
27	2.2127973446431863E-4	0.0	0.0	0.027881246542504148	0.0
28	2.2127973446431863E-4	0.0	0.0	0.04698506361792366	0.0
29	2.2127973446431863E-4	0.0	0.0	0.07907062511524986	0.0
30	2.2127973446431863E-4	0.0	0.0	0.13328416005900792	0.0
31	2.2127973446431863E-4	0.0	0.0	0.24495666605200073	0.0
32	2.2127973446431863E-4	0.0	0.0	0.3955006453992255	0.0
33	2.2127973446431863E-4	0.0	0.0	0.5216300940438872	0.0
34	2.2127973446431863E-4	0.0	0.0	0.6830167803798636	0.0
35	2.2127973446431863E-4	0.0	0.0	0.8568688917573299	0.0
36	2.2127973446431863E-4	0.0	0.0	1.093048128342246	0.0
37	2.2127973446431863E-4	0.0	0.0	1.4785911856905771	0.0
38	2.2127973446431863E-4	0.0	0.0	1.884860778167066	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	40	1.8202878E-5	32.999863	4
ATACATG	305	0.0	26.692348	1
TAGGACC	1240	0.0	24.483768	4
ATAGGAC	225	0.0	24.444342	3
ACGGTAA	65	1.8136605E-5	23.69221	38
ACTGTTC	910	0.0	20.549364	8
TATAGGA	295	0.0	20.136251	2
AGTACGT	110	1.2659802E-7	19.999914	5
AGGACCT	2055	0.0	19.912325	5
TTGCGAG	100	1.123688E-6	19.799917	18
TGTAGGA	3535	0.0	19.542366	2
AACTTGC	215	0.0	19.44178	7
TTAGGAC	2055	0.0	19.377048	3
CTGTAGG	3345	0.0	19.273315	1
TGTCCAC	1440	0.0	19.24992	10
GTCCTAA	1770	0.0	19.143988	1
TCCTACA	3210	0.0	19.053583	2
GTAGTAC	185	1.8189894E-12	19.026949	3
TAGGACA	845	0.0	19.005838	4
GGACCTG	2015	0.0	18.997438	6
>>END_MODULE