FastQCFastQC Report
Thu 2 Feb 2017
SRR4062714_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062714_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028299
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34560.3360890169104511No Hit
GTACATGGAAGCAGTGGTATCAACG25770.2506080429913867No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23360.22717127994873088No Hit
TCCATGTACTCTGCGTTGATACCAC21350.2076244360832793No Hit
TATCAACGCAGAGTACTTTTTTTTT20860.20285928509120402No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18240.1773803144805159No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14360.1396480984616342No Hit
GGTATCAACGCAGAGTACTTTTTTT11440.11125168846804286No Hit
GAGTACATGGAAGCAGTGGTATCAA10910.10609754555824717No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAC307.7128434E-419.00272818
TAATACG307.730542E-418.9962524
CTCGACG402.7579785E-416.62657513
TCGACGC508.702315E-515.20144114
AACCGCG400.00527417314.251357
CGACGCT400.00527417314.2513515
CGGCTCG707.246059E-613.5733778
CGCATCG500.001497873113.3012613
TTAAGGT500.001501173813.2973774
AGAACCG801.9997624E-613.0611945
ACGCTAT905.3864096E-712.66910110
CGCGGTC550.003062815412.09323410
GACAGTC550.0030650512.0920557
TAACCCT550.00307176312.0885254
GTAAGCC550.003077366912.0855833
CGGTCCA1302.6011548E-911.69455510
GACGCTT658.012979E-411.69341716
CGACCCG658.0260815E-411.6911395
TAGGACC3300.011.512884
GATATAC2400.011.4735521
Produced by FastQC (version 0.11.5)