##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062714_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1028299
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.081298338323776	32.0	32.0	32.0	32.0	32.0
2	30.764496513173697	32.0	32.0	32.0	32.0	32.0
3	30.75856827634764	32.0	32.0	32.0	32.0	32.0
4	30.812974630919605	32.0	32.0	32.0	32.0	32.0
5	30.643218558026412	32.0	32.0	32.0	32.0	32.0
6	34.26704295151507	36.0	36.0	36.0	32.0	36.0
7	34.125547141444265	36.0	36.0	36.0	32.0	36.0
8	34.123205410099594	36.0	36.0	36.0	32.0	36.0
9	34.26377347444664	36.0	36.0	36.0	32.0	36.0
10	33.94577160923039	36.0	36.0	36.0	32.0	36.0
11	34.283911586027024	36.0	36.0	36.0	32.0	36.0
12	34.06058938110414	36.0	36.0	36.0	32.0	36.0
13	34.17904519988836	36.0	36.0	36.0	32.0	36.0
14	34.09306145391564	36.0	36.0	36.0	32.0	36.0
15	34.03917732099321	36.0	36.0	36.0	32.0	36.0
16	34.035950633035725	36.0	36.0	36.0	32.0	36.0
17	33.9741135603555	36.0	36.0	36.0	32.0	36.0
18	33.98420692814055	36.0	36.0	36.0	32.0	36.0
19	33.98060194554308	36.0	36.0	36.0	32.0	36.0
20	33.94162495538749	36.0	36.0	36.0	32.0	36.0
21	33.911936119747274	36.0	36.0	36.0	32.0	36.0
22	33.875747229161945	36.0	36.0	36.0	32.0	36.0
23	33.83489043556398	36.0	36.0	36.0	32.0	36.0
24	33.82197104149669	36.0	36.0	36.0	32.0	36.0
25	33.356112375875114	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	36.0
5	103.0
6	147.0
7	48.0
8	155.0
9	185.0
10	117.0
11	40.0
12	68.0
13	65.0
14	239.0
15	287.0
16	450.0
17	587.0
18	766.0
19	1152.0
20	1720.0
21	2466.0
22	3584.0
23	5347.0
24	7060.0
25	9607.0
26	13046.0
27	16750.0
28	22100.0
29	29025.0
30	36945.0
31	50370.0
32	71946.0
33	102742.0
34	226334.0
35	424804.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.559768955680845	17.47985072957525	11.09014677091347	25.87023354383044
2	16.215580136502705	20.54863662621638	37.746557770520624	25.48922546676029
3	19.633453116718293	24.498636025460858	28.32515596963759	27.542754888183264
4	12.594369260277277	15.613310223685056	36.32056661433927	35.471753901698406
5	13.932018953989589	37.13869078945448	34.0485990254513	14.88069123110462
6	33.177636469063636	35.63158898362799	17.47673789611734	13.714036651191028
7	29.120852921456454	31.038388666638774	21.11858360232031	18.72217480958447
8	27.618712059200547	33.512195074472736	20.0906539954819	18.77843887084482
9	27.539178850234585	14.431152296221308	18.83884595168104	39.190822901863065
10	15.395018167798016	27.372426228614376	32.679156297585806	24.5533993060018
11	36.79170240669541	21.50662111415422	22.54323610422815	19.158440374922233
12	24.14116116568384	24.05225868770984	29.84367575231166	21.96290439429466
13	29.822487741995786	20.29171908042575	25.360340536898633	24.525452640679827
14	22.880525006450608	20.208853641794096	25.90213575973555	31.008485592019746
15	24.558824388448723	27.9590358489868	23.373885986552978	24.108253776011495
16	24.690166925307107	26.2907845469963	25.270890975752703	23.74815755194389
17	23.134945246679603	26.22531670465532	26.41663932327291	24.223098725392163
18	23.633904523758055	25.422745180519936	28.030882842670437	22.912467453051573
19	24.830081677644007	25.74228502654426	26.161773927810515	23.265859368001216
20	24.704380667152833	25.136673738488007	26.70422293859978	23.45472265575938
21	25.40048997460545	24.972638540514435	25.15939754155826	24.467473943321856
22	24.64283592512045	25.733928468818206	25.902905132507897	23.720330473553446
23	23.575060912311592	25.463001735343553	26.260558620918	24.701378731426857
24	23.78179049191885	25.932803483686772	26.36271395714333	23.92269206725105
25	23.99861730510818	25.57235778690919	26.416580653956263	24.01244425402637
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	243.0
1	243.0
2	527.5
3	812.0
4	812.0
5	812.0
6	2209.5
7	3607.0
8	3607.0
9	3607.0
10	3799.5
11	3992.0
12	3992.0
13	3992.0
14	3950.0
15	3908.0
16	3908.0
17	3908.0
18	6414.5
19	8921.0
20	8921.0
21	8921.0
22	14097.0
23	19273.0
24	19273.0
25	19273.0
26	28013.0
27	36753.0
28	36753.0
29	36753.0
30	46266.5
31	55780.0
32	55780.0
33	55780.0
34	64192.0
35	72604.0
36	72604.0
37	72604.0
38	80107.5
39	87611.0
40	87611.0
41	87611.0
42	98044.5
43	108478.0
44	108478.0
45	108478.0
46	123547.0
47	138616.0
48	138616.0
49	138616.0
50	135370.5
51	132125.0
52	132125.0
53	132125.0
54	119852.5
55	107580.0
56	107580.0
57	107580.0
58	97577.5
59	87575.0
60	87575.0
61	87575.0
62	76963.0
63	66351.0
64	66351.0
65	66351.0
66	55347.0
67	44343.0
68	44343.0
69	44343.0
70	33966.5
71	23590.0
72	23590.0
73	23590.0
74	18459.5
75	13329.0
76	13329.0
77	13329.0
78	10304.0
79	7279.0
80	7279.0
81	7279.0
82	5178.0
83	3077.0
84	3077.0
85	3077.0
86	2241.0
87	1405.0
88	1405.0
89	1405.0
90	929.5
91	454.0
92	454.0
93	454.0
94	286.5
95	119.0
96	119.0
97	119.0
98	296.5
99	474.0
100	474.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009530301984150525
2	0.003987167156634403
3	0.005251390889225799
4	0.015267932770526859
5	0.032675321088516084
6	0.043469846805258006
7	0.06593413005361282
8	0.08518922998077408
9	0.09326081227347298
10	0.11737831117213963
11	0.1172810631927095
12	0.12943706062147295
13	0.11815629500758049
14	0.12292144599965575
15	0.11251591220063424
16	0.11018196069431169
17	0.12039299853447295
18	0.12846458082717188
19	0.12982605253919335
20	0.1184480389458708
21	0.12710310911515035
22	0.1487894085280643
23	0.13741139493474175
24	0.13040954041577402
25	0.12827008486831165
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1028299.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.20512271221706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.69713206127979	47.18478483499838
2	13.153143380932834	15.574669358390278
3	3.5417314667635305	6.29065938310366
4	1.3354401925189467	3.1625960189164406
5	0.602010284760948	1.782104639164433
6	0.3518916969588241	1.2500274659914485
7	0.21016366770530798	0.8709936015299659
8	0.1606242798393871	0.7607824158761923
9	0.10164682718908524	0.5416211589333583
>10	0.6832770315452865	7.828358801912756
>50	0.0868328498568954	3.661134596164713
>100	0.0701759033354865	7.6643853794651635
>500	0.004447767985182956	1.6640546442037312
>1k	0.0014825893283943186	1.7638277013494594
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3456	0.3360890169104511	No Hit
GTACATGGAAGCAGTGGTATCAACG	2577	0.2506080429913867	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2336	0.22717127994873088	No Hit
TCCATGTACTCTGCGTTGATACCAC	2135	0.2076244360832793	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2086	0.20285928509120402	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1824	0.1773803144805159	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1436	0.1396480984616342	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1144	0.11125168846804286	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1091	0.10609754555824717	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	9.72479794301074E-5	0.0
11	0.0	0.0	0.0	9.72479794301074E-5	0.0
12	0.0	0.0	0.0	9.72479794301074E-5	1.944959588602148E-4
13	0.0	0.0	0.0	9.72479794301074E-5	2.917439382903222E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTAC	30	7.7128434E-4	19.002728	18
TAATACG	30	7.730542E-4	18.996252	4
CTCGACG	40	2.7579785E-4	16.626575	13
TCGACGC	50	8.702315E-5	15.201441	14
AACCGCG	40	0.005274173	14.25135	7
CGACGCT	40	0.005274173	14.25135	15
CGGCTCG	70	7.246059E-6	13.573377	8
CGCATCG	50	0.0014978731	13.30126	13
TTAAGGT	50	0.0015011738	13.297377	4
AGAACCG	80	1.9997624E-6	13.061194	5
ACGCTAT	90	5.3864096E-7	12.669101	10
CGCGGTC	55	0.0030628154	12.093234	10
GACAGTC	55	0.00306505	12.092055	7
TAACCCT	55	0.003071763	12.088525	4
GTAAGCC	55	0.0030773669	12.085583	3
CGGTCCA	130	2.6011548E-9	11.694555	10
GACGCTT	65	8.012979E-4	11.693417	16
CGACCCG	65	8.0260815E-4	11.691139	5
TAGGACC	330	0.0	11.51288	4
GATATAC	240	0.0	11.473552	1
>>END_MODULE