FastQCFastQC Report
Thu 2 Feb 2017
SRR4062714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028299
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA11290.10979296877659124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA6800.021.3538862
GTAGGAC7300.020.794433
CACGCGA550.0044832819.99991210
TAGGACG4800.019.7082464
GTATTAG2550.018.9830781
GCGTTCT600.007411557718.33325211
CTGTAGG7850.018.2191371
CGCTTCG1456.3046173E-918.20681832
TCAATAC851.4306176E-418.1175673
CGAAAAC2200.017.9999223
ACGGTAT1852.1827873E-1117.8377599
GATATAC3750.017.602491
CGCAATA1751.9099389E-1017.59992236
GTAAACG1651.6589183E-917.33325827
TACACCG902.2150412E-417.1110345
GGACGTG5550.017.044976
AGGACGT5350.016.8597375
CCGCTAG2350.016.8509936
AACCGCG2500.016.71992541
CGGTCCA1854.2200554E-1016.64857510
Produced by FastQC (version 0.11.5)