##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062714_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1028299
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22908414770412	32.0	32.0	32.0	32.0	32.0
2	31.413369068724176	32.0	32.0	32.0	32.0	32.0
3	31.506060007838187	32.0	32.0	32.0	32.0	32.0
4	31.607785284241256	32.0	32.0	32.0	32.0	32.0
5	31.553514104360698	32.0	32.0	32.0	32.0	32.0
6	35.13790152475107	36.0	36.0	36.0	36.0	36.0
7	35.15265112579123	36.0	36.0	36.0	36.0	36.0
8	35.08969959126674	36.0	36.0	36.0	36.0	36.0
9	35.208991742674066	36.0	36.0	36.0	36.0	36.0
10	35.10587776512474	36.0	36.0	36.0	36.0	36.0
11	35.22194517353416	36.0	36.0	36.0	36.0	36.0
12	35.14322876906425	36.0	36.0	36.0	36.0	36.0
13	35.169879577827075	36.0	36.0	36.0	36.0	36.0
14	35.14627068586083	36.0	36.0	36.0	36.0	36.0
15	35.0982389363405	36.0	36.0	36.0	36.0	36.0
16	35.11330459331381	36.0	36.0	36.0	36.0	36.0
17	35.09563755289074	36.0	36.0	36.0	36.0	36.0
18	35.111071779706094	36.0	36.0	36.0	36.0	36.0
19	35.08508711960237	36.0	36.0	36.0	36.0	36.0
20	35.059650938102635	36.0	36.0	36.0	36.0	36.0
21	35.04827778690828	36.0	36.0	36.0	36.0	36.0
22	35.03360112185269	36.0	36.0	36.0	36.0	36.0
23	35.002342703824475	36.0	36.0	36.0	36.0	36.0
24	34.98735776267409	36.0	36.0	36.0	36.0	36.0
25	34.958312708657694	36.0	36.0	36.0	36.0	36.0
26	34.913049609111745	36.0	36.0	36.0	32.0	36.0
27	34.897057178894464	36.0	36.0	36.0	32.0	36.0
28	34.87842154859627	36.0	36.0	36.0	32.0	36.0
29	34.84997359717359	36.0	36.0	36.0	32.0	36.0
30	34.82833883918977	36.0	36.0	36.0	32.0	36.0
31	34.822550639454086	36.0	36.0	36.0	32.0	36.0
32	34.78769307370716	36.0	36.0	36.0	32.0	36.0
33	34.76165103729557	36.0	36.0	36.0	32.0	36.0
34	34.733805050865556	36.0	36.0	36.0	32.0	36.0
35	34.69351715794725	36.0	36.0	36.0	32.0	36.0
36	34.65020096294949	36.0	36.0	36.0	32.0	36.0
37	34.63178511308481	36.0	36.0	36.0	32.0	36.0
38	34.58734959384381	36.0	36.0	36.0	32.0	36.0
39	34.55026991176691	36.0	36.0	36.0	32.0	36.0
40	34.53840176835726	36.0	36.0	36.0	32.0	36.0
41	34.50339735816139	36.0	36.0	36.0	32.0	36.0
42	34.46301610718283	36.0	36.0	36.0	32.0	36.0
43	34.44900753574593	36.0	36.0	36.0	32.0	36.0
44	34.411355063070175	36.0	36.0	36.0	32.0	36.0
45	34.34483355522081	36.0	36.0	36.0	32.0	36.0
46	34.337541901723135	36.0	36.0	36.0	32.0	36.0
47	34.2869700349801	36.0	36.0	36.0	32.0	36.0
48	34.2442412177781	36.0	36.0	36.0	32.0	36.0
49	34.23153868670494	36.0	36.0	36.0	32.0	36.0
50	33.70611563368242	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	9.0
21	31.0
22	113.0
23	287.0
24	757.0
25	1723.0
26	3553.0
27	6779.0
28	11461.0
29	17241.0
30	24680.0
31	34287.0
32	50622.0
33	79846.0
34	171549.0
35	625355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.080661617453536	18.117642710624065	11.568287414153811	26.233408257768588
2	15.670857245110426	21.037890259166222	37.45272348698139	25.83852900874196
3	19.121828734138806	24.689972537090487	28.66412005881575	27.524078669954953
4	12.361056273789403	16.148105502900922	36.38881487201131	35.102023351298364
5	13.784803836238293	37.34225162136694	34.015009253145244	14.857935289249527
6	33.43479588522349	35.835276681571614	17.100459596185527	13.629467837019375
7	29.131604718082972	30.98787414944486	21.179637440083088	18.70088369238908
8	27.60306097740054	33.66890369435349	20.005465336443972	18.722569991801997
9	27.63573629848906	14.161153516632808	18.73540672508677	39.467703459791366
10	15.399898278613517	27.43871189216366	32.713539544432116	24.447850284790707
11	36.98389281716699	21.394360978664768	22.525354979436916	19.09639122473133
12	24.123431025411872	24.029781221220674	29.84375167144965	22.003036081917806
13	30.087552355880927	20.05875722917167	25.36412074698118	24.489569667966222
14	22.914735889075065	20.11088214614621	25.894413978813557	31.079967985965173
15	24.81875407833714	27.86232409056121	23.189655926923976	24.12926590417768
16	24.719366643359567	26.18917260446621	25.261524128682417	23.829936623491804
17	23.2743589170076	26.152801860159354	26.422373259139608	24.150465963693442
18	23.85249815471959	25.34583812684832	27.84802863758498	22.953635080847107
19	24.943134244028244	25.625037075792157	26.171084480292212	23.260744199887387
20	24.887800143732512	25.031240913391922	26.666368439529748	23.414590503345817
21	25.511062443900073	24.82021279802859	25.25471677012231	24.41400798794903
22	24.73123089685004	25.618132469252618	25.876714846557274	23.773921787340065
23	23.6874683336267	25.330083954180644	26.251605807260344	24.730841904932323
24	23.976781072431265	25.841511078003577	26.22709931644395	23.954608533121203
25	24.254035061786503	25.274555357926047	26.399617231952966	24.071792348334483
26	23.207257811200826	25.986410567354433	26.83664965151187	23.969681969932868
27	24.381429914839945	25.820797258384964	26.20453778521617	23.593235041558923
28	23.50931003531074	25.697097828549868	26.418872331880124	24.374719804259268
29	23.52700916756702	25.783648530242665	26.429764105576297	24.259578196614022
30	23.97765630424614	25.703710691151116	26.60588019632422	23.712752808278527
31	23.862514696600893	25.558519457861966	26.120807274926843	24.4581585706103
32	23.605293791008258	26.1694312646419	26.21338735134431	24.011887593005536
33	23.568047814886526	25.528372584238628	26.62396832049822	24.279611280376624
34	23.72520054964558	25.921643413053985	26.926993024402435	23.426163012897998
35	24.40369970212944	25.724035518852006	26.516801047166243	23.35546373185231
36	23.4823723450086	26.034840061110632	26.469927521080933	24.01286007279984
37	24.25977269257288	25.895386458607856	25.90394428079771	23.940896568021557
38	23.763030013643892	25.886050652582565	26.124891690062913	24.226027643710633
39	24.01470778440901	25.510284460064632	26.164666113649826	24.310341641876537
40	24.52370370874619	25.678620712458144	26.391545649660266	23.406129929135396
41	23.493264118704772	25.542862533173714	27.04903923858722	23.91483410953429
42	24.345934402347954	26.22457086897877	26.317248193375665	23.11224653529761
43	23.49861275757343	25.501532141915924	26.572621387359124	24.427233713151526
44	23.157175101794323	25.840538598209278	26.682317108156283	24.319969191840116
45	23.355949971749464	26.042522651485612	26.455632068104705	24.145895308660222
46	23.259233470485665	25.357946196478252	26.5072250526842	24.875595280351884
47	23.675140863835193	25.4721880233162	26.73048085282671	24.1221902600219
48	23.60733599857629	26.29964630909881	25.893247003060395	24.199770689264504
49	23.14550534426271	25.7714925328139	26.764588898754155	24.318413224169237
50	22.872844253319563	26.77881314560565	25.79427364441047	24.554068956664313
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	53.0
1	46.0
2	39.0
3	140.0
4	241.0
5	286.0
6	331.0
7	295.5
8	260.0
9	349.0
10	438.0
11	644.0
12	850.0
13	1415.0
14	1980.0
15	2681.0
16	3382.0
17	4007.0
18	4632.0
19	5044.0
20	5456.0
21	6310.5
22	7165.0
23	7614.0
24	8063.0
25	8998.0
26	9933.0
27	12409.5
28	14886.0
29	17067.5
30	19249.0
31	21174.0
32	23099.0
33	26868.5
34	30638.0
35	34984.5
36	39331.0
37	43264.5
38	47198.0
39	48736.0
40	50274.0
41	52686.0
42	55098.0
43	56257.5
44	57417.0
45	62169.0
46	66921.0
47	69718.0
48	72515.0
49	72766.5
50	73018.0
51	70075.0
52	67132.0
53	64486.0
54	61840.0
55	61844.0
56	61848.0
57	60359.5
58	58871.0
59	53705.0
60	48539.0
61	42776.5
62	37014.0
63	32715.5
64	28417.0
65	24404.5
66	20392.0
67	18530.5
68	16669.0
69	14909.0
70	13149.0
71	10421.5
72	7694.0
73	6691.5
74	5689.0
75	4328.5
76	2968.0
77	2526.5
78	2085.0
79	1787.5
80	1490.0
81	1184.0
82	878.0
83	726.5
84	575.0
85	449.5
86	324.0
87	249.0
88	174.0
89	111.0
90	48.0
91	32.5
92	17.0
93	12.5
94	8.0
95	5.5
96	3.0
97	4.5
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014781692873376323
2	0.0038899191772042954
3	2.917439382903222E-4
4	4.8623989715053693E-4
5	0.0
6	4.8623989715053693E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.9449595886021477E-4
47	9.724797943010738E-5
48	0.0
49	0.0
50	9.724797943010738E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1028299.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.729766760217835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68921895463811	51.42305104043234
2	12.713192789106408	16.204176224548693
3	3.3390608873819296	6.383927146530491
4	1.204670061167614	3.070933680849176
5	0.5773124804839733	1.8395994864503213
6	0.3409588842034897	1.3037538099067512
7	0.18668459558102382	0.8328156013872968
8	0.14079650070908142	0.7178342520675682
9	0.098952675036637	0.5675607810346055
>10	0.581349334155347	7.197912418220802
>50	0.07089391372687029	3.1900900942094115
>100	0.055068204318569694	6.442297238236244
>500	0.001533932909116545	0.6176299044574938
>1k	3.06786581823309E-4	0.2084183216687839
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1129	0.10979296877659124	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	9.72479794301074E-5
11	0.0	0.0	0.0	0.0	9.72479794301074E-5
12	0.0	0.0	0.0	0.0	9.72479794301074E-5
13	0.0	0.0	0.0	0.0	9.72479794301074E-5
14	0.0	0.0	0.0	0.0	9.72479794301074E-5
15	0.0	0.0	0.0	0.0	9.72479794301074E-5
16	0.0	0.0	0.0	9.72479794301074E-5	1.944959588602148E-4
17	0.0	0.0	0.0	9.72479794301074E-5	1.944959588602148E-4
18	0.0	0.0	0.0	2.917439382903222E-4	1.944959588602148E-4
19	0.0	0.0	0.0	3.889919177204296E-4	1.944959588602148E-4
20	0.0	0.0	0.0	5.834878765806444E-4	1.944959588602148E-4
21	0.0	0.0	0.0	0.001264223732591396	2.917439382903222E-4
22	0.0	0.0	0.0	0.0018477116091720404	2.917439382903222E-4
23	0.0	0.0	0.0	0.003792671197774188	2.917439382903222E-4
24	0.0	0.0	0.0	0.007488094416118269	2.917439382903222E-4
25	0.0	0.0	0.0	0.00933580602529031	2.917439382903222E-4
26	0.0	0.0	0.0	0.012155997428763424	2.917439382903222E-4
27	0.0	0.0	0.0	0.016143164585397825	2.917439382903222E-4
28	0.0	0.0	0.0	0.02645145040498921	2.917439382903222E-4
29	0.0	0.0	0.0	0.04580379831158058	2.917439382903222E-4
30	0.0	0.0	0.0	0.08061857494755903	2.917439382903222E-4
31	0.0	0.0	0.0	0.15209583982868796	2.917439382903222E-4
32	0.0	0.0	0.0	0.24243921271925772	2.917439382903222E-4
33	0.0	0.0	0.0	0.328698170473763	2.917439382903222E-4
34	0.0	0.0	0.0	0.42857184534848325	2.917439382903222E-4
35	0.0	0.0	0.0	0.5459501565206228	2.917439382903222E-4
36	0.0	0.0	0.0	0.6993102200819022	2.917439382903222E-4
37	0.0	0.0	0.0	0.9322191308170095	2.917439382903222E-4
38	0.0	0.0	0.0	1.2172529585266543	2.917439382903222E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAGGA	680	0.0	21.353886	2
GTAGGAC	730	0.0	20.79443	3
CACGCGA	55	0.00448328	19.999912	10
TAGGACG	480	0.0	19.708246	4
GTATTAG	255	0.0	18.983078	1
GCGTTCT	60	0.0074115577	18.333252	11
CTGTAGG	785	0.0	18.219137	1
CGCTTCG	145	6.3046173E-9	18.206818	32
TCAATAC	85	1.4306176E-4	18.117567	3
CGAAAAC	220	0.0	17.99992	23
ACGGTAT	185	2.1827873E-11	17.837759	9
GATATAC	375	0.0	17.60249	1
CGCAATA	175	1.9099389E-10	17.599922	36
GTAAACG	165	1.6589183E-9	17.333258	27
TACACCG	90	2.2150412E-4	17.111034	5
GGACGTG	555	0.0	17.04497	6
AGGACGT	535	0.0	16.859737	5
CCGCTAG	235	0.0	16.85099	36
AACCGCG	250	0.0	16.719925	41
CGGTCCA	185	4.2200554E-10	16.648575	10
>>END_MODULE