FastQCFastQC Report
Thu 2 Feb 2017
SRR4062713_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062713_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685242
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43070.16039522694788774No Hit
GTACATGGAAGCAGTGGTATCAACG37160.138386037459566No Hit
GTCTTGCGCCGGTCCAAGAATTTCA30570.11384448775939003No Hit
TCCATGTACTCTGCGTTGATACCAC30290.11280175120156768No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG30160.1123176235140073No Hit
GAATAGGACCGCGGTTCTATTTTGT29630.11034387217241501No Hit
GCGCAAGACGGACCAGAGCGAAAGC28900.10762530900380674No Hit
GGGTAGGCACACGCTGAGCCAGTCA28440.10591224180167003No Hit
TATCAACGCAGAGTACTTTTTTTTT28060.10449709933033968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCA5550.012.1535839
CGGTCCA6050.011.77727610
CGTCGTA4850.011.75299410
TCGCGTA2300.011.5656269
CAAGACG6100.011.5222044
CGCCGGT5900.011.432187
CGCCAGT4250.011.40359318
CGAACGA2000.011.40274216
GCGTAAC2500.011.4001911
CGCAAGA6050.011.30112652
CCGTCGT5050.011.2875289
CGGTCGG2950.011.271584510
CGCGTAA2450.011.24529610
AAGACGG6350.011.2187775
TCGGCGT3000.011.08475813
CCGACCA4950.010.9397819
AGAATTT6200.010.88205417
TGTCCGA700.001491573610.85752710
GTCGTAG5450.010.8075211
AATTCCG2550.010.8022895
Produced by FastQC (version 0.11.5)