##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062713_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2685242
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04435875798159	32.0	32.0	32.0	32.0	32.0
2	30.49946596992003	32.0	32.0	32.0	32.0	32.0
3	30.489586413440577	32.0	32.0	32.0	32.0	32.0
4	30.585998952794572	32.0	32.0	32.0	32.0	32.0
5	30.259475309860342	32.0	32.0	32.0	27.0	32.0
6	33.94310903821704	36.0	36.0	36.0	32.0	36.0
7	33.812899172588544	36.0	36.0	36.0	32.0	36.0
8	33.77258809448087	36.0	36.0	36.0	32.0	36.0
9	34.041545976116865	36.0	36.0	36.0	32.0	36.0
10	33.52247693131569	36.0	36.0	36.0	27.0	36.0
11	34.03344205103301	36.0	36.0	36.0	32.0	36.0
12	33.69866514824362	36.0	36.0	36.0	27.0	36.0
13	33.877544370302566	36.0	36.0	36.0	32.0	36.0
14	33.72800477573343	36.0	36.0	36.0	32.0	36.0
15	33.64000488596559	36.0	36.0	36.0	27.0	36.0
16	33.64710219786522	36.0	36.0	36.0	27.0	36.0
17	33.536822007104014	36.0	36.0	36.0	27.0	36.0
18	33.557251450707234	36.0	36.0	36.0	27.0	36.0
19	33.55718627967237	36.0	36.0	36.0	27.0	36.0
20	33.55063863890108	36.0	36.0	36.0	27.0	36.0
21	33.54068758048623	36.0	36.0	36.0	27.0	36.0
22	33.4851622311881	36.0	36.0	36.0	27.0	36.0
23	33.42871331522448	36.0	36.0	36.0	27.0	36.0
24	33.3960305998491	36.0	36.0	36.0	27.0	36.0
25	32.88776207135148	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	101.0
5	271.0
6	460.0
7	119.0
8	371.0
9	473.0
10	310.0
11	118.0
12	194.0
13	173.0
14	507.0
15	631.0
16	958.0
17	1395.0
18	2009.0
19	3278.0
20	5300.0
21	8062.0
22	12409.0
23	18370.0
24	24933.0
25	34091.0
26	45359.0
27	56450.0
28	72844.0
29	92507.0
30	115107.0
31	151110.0
32	210459.0
33	289332.0
34	589445.0
35	948085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.44441510450089	17.42237712388189	11.622304796162087	25.51090297545513
2	16.76288092335893	19.38023643345463	37.80719385450289	26.04968878868355
3	19.368953498288906	23.480826046589232	27.766396151839373	29.38382430328249
4	12.5925658305914	15.228731927268827	35.62092574397191	36.55777649816787
5	14.828971279513434	36.40647829627692	33.280954413948436	15.483596010261202
6	34.8802481660829	34.88047171227816	16.60944505029603	13.629835071342905
7	30.88224114621142	30.117600850237974	20.26227844101176	18.737879562538847
8	28.06978177911929	33.23401213174807	19.105229533359324	19.590976555773317
9	26.955609944839527	15.012722172065793	18.570382723789827	39.46128515930486
10	15.998110434443486	26.590117139642178	31.169107169089273	26.242665256825067
11	37.41503700527581	21.475326709047184	21.72506105404448	19.38457523163252
12	24.7686535217318	23.640522476108366	28.26461800982365	23.32620599233618
13	29.163211585211258	19.420515726834797	25.231353745656293	26.184918942297653
14	23.47819051077853	19.850248293564356	24.817312761893966	31.85424843376315
15	25.245394106962305	27.013409807412973	22.189264399287755	25.55193168633697
16	26.075047431715664	25.93807425556719	23.354411196159084	24.63246711655806
17	24.169184402676684	26.18583408557804	24.881980541936848	24.76300096980842
18	25.152074375397827	24.907196689322298	25.82917031323238	24.111558622047493
19	25.439617378035706	25.458001191032427	25.03118349618359	24.071197934748277
20	25.534213886895163	24.502157884434336	24.986232417669484	24.977395811001017
21	26.64250860062451	24.492706715161784	24.24063551772665	24.62414916648706
22	25.574778234288427	24.464532404796643	24.863419771983374	25.09726958893156
23	24.149837444518944	24.33301535755866	25.3992286539183	26.11791854400409
24	24.47569867067612	25.33456838738932	25.037588601704012	25.152144340230542
25	24.897781566338782	24.717302872706213	24.940701189555952	25.44421437139905
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	389.0
1	389.0
2	794.0
3	1199.0
4	1199.0
5	1199.0
6	2669.5
7	4140.0
8	4140.0
9	4140.0
10	4668.0
11	5196.0
12	5196.0
13	5196.0
14	6669.5
15	8143.0
16	8143.0
17	8143.0
18	13018.5
19	17894.0
20	17894.0
21	17894.0
22	28310.0
23	38726.0
24	38726.0
25	38726.0
26	56422.5
27	74119.0
28	74119.0
29	74119.0
30	91312.5
31	108506.0
32	108506.0
33	108506.0
34	137014.0
35	165522.0
36	165522.0
37	165522.0
38	189985.5
39	214449.0
40	214449.0
41	214449.0
42	246528.5
43	278608.0
44	278608.0
45	278608.0
46	318598.0
47	358588.0
48	358588.0
49	358588.0
50	360673.0
51	362758.0
52	362758.0
53	362758.0
54	337595.0
55	312432.0
56	312432.0
57	312432.0
58	288085.5
59	263739.0
60	263739.0
61	263739.0
62	231918.0
63	200097.0
64	200097.0
65	200097.0
66	164388.5
67	128680.0
68	128680.0
69	128680.0
70	97124.5
71	65569.0
72	65569.0
73	65569.0
74	51277.5
75	36986.0
76	36986.0
77	36986.0
78	30044.0
79	23102.0
80	23102.0
81	23102.0
82	15989.0
83	8876.0
84	8876.0
85	8876.0
86	6546.5
87	4217.0
88	4217.0
89	4217.0
90	2945.5
91	1674.0
92	1674.0
93	1674.0
94	1022.5
95	371.0
96	371.0
97	371.0
98	816.5
99	1262.0
100	1262.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008974982515542362
2	0.0033144126302210378
3	0.00428266800534179
4	0.01474727417491608
5	0.035602005331363054
6	0.04591764913553416
7	0.0654689595947032
8	0.08803675795328689
9	0.0939952525694146
10	0.11708441920690947
11	0.11887196759174778
12	0.13220409929533353
13	0.11924437350525577
14	0.12423461274626271
15	0.11269002942751527
16	0.1104183533551166
17	0.12173949312575924
18	0.13015586677103963
19	0.13164549042507157
20	0.11980298237551773
21	0.1288524460737617
22	0.15022854550911985
23	0.13764122563255005
24	0.1332468358531559
25	0.12978346085753165
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2685242.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.775639413648385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.31593290109402	39.25765929915784
2	13.05339575492087	13.255890319510216
3	4.199772128000606	6.397383455725486
4	1.816250525140144	3.688851269974619
5	0.9633031801260664	2.4456167460050966
6	0.591013491724318	1.8005452766637144
7	0.40455826590526534	1.4379193241991644
8	0.27228199515921553	1.1060233924026468
9	0.21026585808823192	0.9608745052160482
>10	1.0113771746246087	9.091437494313984
>50	0.0710977622033393	2.5358671779779756
>100	0.07177640094021512	7.957809691688499
>500	0.011914259906537104	4.175005280466634
>1k	0.007060302166832086	5.889116766698047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4307	0.16039522694788774	No Hit
GTACATGGAAGCAGTGGTATCAACG	3716	0.138386037459566	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	3057	0.11384448775939003	No Hit
TCCATGTACTCTGCGTTGATACCAC	3029	0.11280175120156768	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3016	0.1123176235140073	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2963	0.11034387217241501	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2890	0.10762530900380674	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2844	0.10591224180167003	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2806	0.10449709933033968	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.448118270159636E-5
2	0.0	0.0	0.0	0.0	7.448118270159636E-5
3	0.0	0.0	0.0	0.0	7.448118270159636E-5
4	0.0	0.0	0.0	3.724059135079818E-5	7.448118270159636E-5
5	0.0	3.724059135079818E-5	0.0	7.448118270159636E-5	7.448118270159636E-5
6	0.0	3.724059135079818E-5	0.0	7.448118270159636E-5	7.448118270159636E-5
7	0.0	3.724059135079818E-5	0.0	1.1172177405239454E-4	7.448118270159636E-5
8	0.0	3.724059135079818E-5	0.0	1.1172177405239454E-4	1.1172177405239454E-4
9	0.0	3.724059135079818E-5	0.0	1.1172177405239454E-4	1.4896236540319272E-4
10	0.0	3.724059135079818E-5	0.0	1.4896236540319272E-4	1.4896236540319272E-4
11	0.0	3.724059135079818E-5	0.0	1.4896236540319272E-4	1.4896236540319272E-4
12	0.0	3.724059135079818E-5	0.0	1.862029567539909E-4	2.2344354810478908E-4
13	0.0	3.724059135079818E-5	0.0	1.862029567539909E-4	2.2344354810478908E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACCA	555	0.0	12.153583	9
CGGTCCA	605	0.0	11.777276	10
CGTCGTA	485	0.0	11.752994	10
TCGCGTA	230	0.0	11.565626	9
CAAGACG	610	0.0	11.522204	4
CGCCGGT	590	0.0	11.43218	7
CGCCAGT	425	0.0	11.403593	18
CGAACGA	200	0.0	11.402742	16
GCGTAAC	250	0.0	11.40019	11
CGCAAGA	605	0.0	11.3011265	2
CCGTCGT	505	0.0	11.287528	9
CGGTCGG	295	0.0	11.2715845	10
CGCGTAA	245	0.0	11.245296	10
AAGACGG	635	0.0	11.218777	5
TCGGCGT	300	0.0	11.084758	13
CCGACCA	495	0.0	10.939781	9
AGAATTT	620	0.0	10.882054	17
TGTCCGA	70	0.0014915736	10.857527	10
GTCGTAG	545	0.0	10.80752	11
AATTCCG	255	0.0	10.802289	5
>>END_MODULE