FastQCFastQC Report
Thu 2 Feb 2017
SRR4062713_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062713_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685242
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC34740.12937381435267287No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA29400.10948733857134665No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT29130.10848184260487509No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT29010.10803495550866553No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC28070.10453433992169048No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCG708.127155E-418.8570385
GGCGTTA8200.017.97551242
CGCAATA9100.017.16474236
ATACGAA9000.017.11101540
ATACCGT7850.016.8151936
TACGAAT9350.016.47049741
CTAGCGG9500.016.44201529
TAGCGGC9650.016.41441730
CGTCTTA3650.016.27388415
TACCGTC8250.016.2665797
TCTAGCG9650.016.1864428
GCGTTAT9000.016.13324443
CGGCGTT9150.016.10920141
GTATCAA56350.016.0886881
CGTTATT8900.016.06732744
CGAATGC9450.016.06340243
GCCGGTC10400.015.8652978
GCGGCGT9300.015.84937640
CGAGCCG10300.015.805737515
AATACGA9750.015.79478439
Produced by FastQC (version 0.11.5)