FastQCFastQC Report
Thu 2 Feb 2017
SRR4062712_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062712_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815147
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG42190.5175753575735419No Hit
TCCATGTACTCTGCGTTGATACCAC36290.4451957745044759No Hit
GAGTACATGGAAGCAGTGGTATCAA19720.24191955561389542No Hit
CATGTACTCTGCGTTGATACCACTG17910.2197149716554192No Hit
GTATCAACGCAGAGTACTTTTTTTT17830.21873355357990643No Hit
GCTTCCATGTACTCTGCGTTGATAC12060.1479487748835486No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11900.14598593873252308No Hit
TATCAACGCAGAGTACTTTTTTTTT11850.14537255243532762No Hit
CCCATGTACTCTGCGTTGATACCAC10560.12954718596768436No Hit
GCGTTGATACCACTGCTTCCATGTA10170.12476277284955967No Hit
GTCTTGCGCCGGTCCAAGAATTTCA9860.12095977780694771No Hit
GTACATGGGAAGCAGTGGTATCAAC9620.11801552358040941No Hit
CATGGAAGCAGTGGTATCAACGCAG9330.11445788305667567No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG8850.1085693746035991No Hit
GCGCAAGACGGACCAGAGCGAAAGC8510.10439834778266989No Hit
GTCCTACAGTGGACATTTCTAAATT8370.10268086615052255No Hit
GAATAGGACCGCGGTTCTATTTTGT8160.10010464370230154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAA753.270543E-916.4681059
ATTCCGA753.270543E-916.4681056
GCGTAAC350.002166851716.28813611
AATTCCG702.5063855E-816.2811345
GGTTAAT807.743438E-915.4303161
CGATAAC755.8273145E-815.20132710
TCGGCGT801.2844612E-714.25124413
CGCGTAA400.005273190314.25124410
CGTACTA400.005290410514.2442433
TCCCGAC1152.5465852E-1114.03694252
ACTAGAC551.9650729E-413.8125993
CGACCCG1051.371518E-913.5676135
GTTAATT852.7089845E-713.4055232
TGCGCGG500.001496798413.30197911
TAGACTG1001.0084477E-813.2962615
CGGTCCA1650.013.2435810
AGAATTT1600.013.06524617
GCGGTCG801.9941126E-613.063649
CGGCTCG801.9941126E-613.0636410
GCGCCGA1102.728484E-912.95726819
Produced by FastQC (version 0.11.5)