##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062712_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	815147
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.179079356238812	32.0	32.0	32.0	32.0	32.0
2	30.795674890541214	32.0	32.0	32.0	32.0	32.0
3	30.771874275437437	32.0	32.0	32.0	32.0	32.0
4	30.869450540822697	32.0	32.0	32.0	32.0	32.0
5	30.612299376676845	32.0	32.0	32.0	32.0	32.0
6	34.273088166919585	36.0	36.0	36.0	32.0	36.0
7	34.1724989480425	36.0	36.0	36.0	32.0	36.0
8	34.15886582420104	36.0	36.0	36.0	32.0	36.0
9	34.35995225401063	36.0	36.0	36.0	32.0	36.0
10	33.97245772848333	36.0	36.0	36.0	32.0	36.0
11	34.32642701255111	36.0	36.0	36.0	32.0	36.0
12	34.07960650042262	36.0	36.0	36.0	32.0	36.0
13	34.22053936283885	36.0	36.0	36.0	32.0	36.0
14	34.10035981240193	36.0	36.0	36.0	32.0	36.0
15	34.031314597244425	36.0	36.0	36.0	32.0	36.0
16	34.027321452449684	36.0	36.0	36.0	32.0	36.0
17	33.94005621072027	36.0	36.0	36.0	32.0	36.0
18	33.961384879046356	36.0	36.0	36.0	32.0	36.0
19	33.951167090107674	36.0	36.0	36.0	32.0	36.0
20	33.944430881791874	36.0	36.0	36.0	32.0	36.0
21	33.91852635168871	36.0	36.0	36.0	32.0	36.0
22	33.8691009106333	36.0	36.0	36.0	32.0	36.0
23	33.800683803044116	36.0	36.0	36.0	32.0	36.0
24	33.76713893322309	36.0	36.0	36.0	32.0	36.0
25	33.2703083002207	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	27.0
5	75.0
6	152.0
7	31.0
8	116.0
9	131.0
10	98.0
11	27.0
12	59.0
13	41.0
14	113.0
15	140.0
16	260.0
17	309.0
18	385.0
19	573.0
20	933.0
21	1436.0
22	2180.0
23	3388.0
24	4969.0
25	6946.0
26	9734.0
27	12971.0
28	17898.0
29	24001.0
30	31562.0
31	43464.0
32	62280.0
33	87896.0
34	186339.0
35	316607.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.03762334693938	16.989028057403026	11.109352590362406	25.863996005295192
2	15.98962601641975	19.859136613684434	37.98134253284195	26.16989483705387
3	19.959171104723417	23.506917479748108	27.91221787515535	28.621693540373126
4	12.428699206044417	14.965283614283104	35.75376702538425	36.85225015428823
5	14.17686163195531	36.81907265393078	33.87354684161632	15.130518872497586
6	34.71032386974516	34.769236056221864	16.825811392634996	13.694628681397985
7	30.016510867096724	30.22298877383795	20.749065509473798	19.011434849591524
8	27.959398666103507	33.680763601716976	19.729365367803787	18.63047236437573
9	26.978149155497704	14.46306108290305	18.60495957071901	39.95383019088023
10	15.521000033160856	26.969110048599077	32.20189311642039	25.30799680181967
11	37.52338118672648	20.921663966751122	21.95319955343929	19.601755293083098
12	24.272928725150106	23.56072070832637	29.3475643910732	22.81878617545032
13	30.052628670036047	19.51178771670177	25.236214911661207	25.199368701600967
14	23.017126602241294	19.950821202399492	25.073694501898864	31.95835769346035
15	25.119157417971433	27.768068882259822	22.765852142868546	24.3469215569002
16	25.567509149411737	25.74276029769361	24.881610296465503	23.80812025642915
17	23.57021788793633	26.23130020388612	26.19248814758407	24.00599376059348
18	24.79680339215809	24.31652608421681	27.872543492119643	23.014127031505456
19	25.08180692582839	25.84939172988483	25.29234306827562	23.776458276011166
20	24.90769922351895	24.428693197868792	26.527353700505778	24.136253878106476
21	25.64620970555143	24.859236585713372	24.41360860401016	25.080945104725032
22	24.815963281111788	25.58024394079374	25.173348338018204	24.43044444007627
23	23.39192421225384	24.98651824244561	25.778717371997008	25.84284017330354
24	24.020857900943398	25.894506682389938	25.67536360062893	24.409271816037737
25	24.55919472814705	24.92473606957077	25.457448703224895	25.058620499057284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	65.0
1	65.0
2	252.5
3	440.0
4	440.0
5	440.0
6	1158.0
7	1876.0
8	1876.0
9	1876.0
10	1893.0
11	1910.0
12	1910.0
13	1910.0
14	2052.5
15	2195.0
16	2195.0
17	2195.0
18	3681.5
19	5168.0
20	5168.0
21	5168.0
22	8622.0
23	12076.0
24	12076.0
25	12076.0
26	18232.5
27	24389.0
28	24389.0
29	24389.0
30	31732.5
31	39076.0
32	39076.0
33	39076.0
34	46067.0
35	53058.0
36	53058.0
37	53058.0
38	59300.0
39	65542.0
40	65542.0
41	65542.0
42	75854.5
43	86167.0
44	86167.0
45	86167.0
46	103138.5
47	120110.0
48	120110.0
49	120110.0
50	114616.5
51	109123.0
52	109123.0
53	109123.0
54	98261.0
55	87399.0
56	87399.0
57	87399.0
58	80243.0
59	73087.0
60	73087.0
61	73087.0
62	64813.5
63	56540.0
64	56540.0
65	56540.0
66	46708.0
67	36876.0
68	36876.0
69	36876.0
70	27743.5
71	18611.0
72	18611.0
73	18611.0
74	14512.5
75	10414.0
76	10414.0
77	10414.0
78	8435.5
79	6457.0
80	6457.0
81	6457.0
82	4474.0
83	2491.0
84	2491.0
85	2491.0
86	1846.0
87	1201.0
88	1201.0
89	1201.0
90	801.0
91	401.0
92	401.0
93	401.0
94	240.5
95	80.0
96	80.0
97	80.0
98	237.5
99	395.0
100	395.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008342053641858463
2	0.0038029950426119465
3	0.0046617358586856115
4	0.013985207576056832
5	0.03484034168070299
6	0.04600397228966064
7	0.06526430202159857
8	0.08476998627241467
9	0.09348007169259041
10	0.11458056031611477
11	0.1133537877217238
12	0.12991521774600162
13	0.11678875098601847
14	0.11961032795311767
15	0.10893740638191639
16	0.1088147291224773
17	0.11862890987760491
18	0.12684828626002426
19	0.12697096351946335
20	0.11789284632097033
21	0.12451741833068146
22	0.14463648887869304
23	0.132982149231979
24	0.1308966358215144
25	0.12414938655236417
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	815147.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.99172453241654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.90645267058117	44.491853581555965
2	11.889139729063352	13.076085938161224
3	3.1030478682405196	5.119258607435551
4	1.2983231845086878	2.855881236662063
5	0.637262658839419	1.7522086294846333
6	0.4301499970238472	1.419281408637312
7	0.28470265026563674	1.0959402801939775
8	0.20473778326283318	0.9007107022853857
9	0.15455892791104303	0.7649515788938733
>10	0.8854918834620019	9.123104507603532
>50	0.09949745103768053	3.7786646826626136
>100	0.09659631753972281	10.273625117489203
>500	0.0078080164276744125	3.0116452877862745
>1k	0.0022308618364784037	2.336788441148436
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4219	0.5175753575735419	No Hit
TCCATGTACTCTGCGTTGATACCAC	3629	0.4451957745044759	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1972	0.24191955561389542	No Hit
CATGTACTCTGCGTTGATACCACTG	1791	0.2197149716554192	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1783	0.21873355357990643	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1206	0.1479487748835486	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1190	0.14598593873252308	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1185	0.14537255243532762	No Hit
CCCATGTACTCTGCGTTGATACCAC	1056	0.12954718596768436	No Hit
GCGTTGATACCACTGCTTCCATGTA	1017	0.12476277284955967	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	986	0.12095977780694771	No Hit
GTACATGGGAAGCAGTGGTATCAAC	962	0.11801552358040941	No Hit
CATGGAAGCAGTGGTATCAACGCAG	933	0.11445788305667567	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	885	0.1085693746035991	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	851	0.10439834778266989	No Hit
GTCCTACAGTGGACATTTCTAAATT	837	0.10268086615052255	No Hit
GAATAGGACCGCGGTTCTATTTTGT	816	0.10010464370230154	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.2267725943909504E-4
6	0.0	0.0	0.0	1.2267725943909504E-4	2.4535451887819007E-4
7	0.0	0.0	0.0	1.2267725943909504E-4	2.4535451887819007E-4
8	0.0	0.0	0.0	1.2267725943909504E-4	2.4535451887819007E-4
9	0.0	0.0	0.0	1.2267725943909504E-4	2.4535451887819007E-4
10	0.0	0.0	0.0	1.2267725943909504E-4	3.680317783172851E-4
11	0.0	0.0	0.0	1.2267725943909504E-4	3.680317783172851E-4
12	0.0	0.0	0.0	1.2267725943909504E-4	7.360635566345702E-4
13	0.0	0.0	0.0	1.2267725943909504E-4	7.360635566345702E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATAA	75	3.270543E-9	16.468105	9
ATTCCGA	75	3.270543E-9	16.468105	6
GCGTAAC	35	0.0021668517	16.288136	11
AATTCCG	70	2.5063855E-8	16.281134	5
GGTTAAT	80	7.743438E-9	15.430316	1
CGATAAC	75	5.8273145E-8	15.201327	10
TCGGCGT	80	1.2844612E-7	14.251244	13
CGCGTAA	40	0.0052731903	14.251244	10
CGTACTA	40	0.0052904105	14.244243	3
TCCCGAC	115	2.5465852E-11	14.0369425	2
ACTAGAC	55	1.9650729E-4	13.812599	3
CGACCCG	105	1.371518E-9	13.567613	5
GTTAATT	85	2.7089845E-7	13.405523	2
TGCGCGG	50	0.0014967984	13.301979	11
TAGACTG	100	1.0084477E-8	13.296261	5
CGGTCCA	165	0.0	13.24358	10
AGAATTT	160	0.0	13.065246	17
GCGGTCG	80	1.9941126E-6	13.06364	9
CGGCTCG	80	1.9941126E-6	13.06364	10
GCGCCGA	110	2.728484E-9	12.957268	19
>>END_MODULE