FastQCFastQC Report
Thu 2 Feb 2017
SRR4062712_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062712_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815147
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA16930.2076926002303879No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16090.19738771043750392No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC9780.11997835973143495No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8750.10734260200920816No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8650.10611582941481719No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8210.10071802999949701No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8170.10022732096174064No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC508.33863E-526.399783
GCCCGTA508.33863E-526.399787
TAACGGC752.063045E-623.46646936
TTAACGG852.3508983E-723.29392235
GTATAGT602.865877E-422.0065651
GTATTAG2200.021.0062681
TGTAGGA5600.020.822532
AGCGAAA2450.020.65288517
CGTCGTA1850.020.21604710
CGTCGTG550.004482598519.99983429
GCGAAAG2550.019.8429718
TATTAGG1001.1219472E-619.801052
ACCGTCG1900.019.6840468
GTAGGAC5600.019.6426933
ATACACT3700.019.6214564
CCTAATA901.0008454E-519.5565912
AAACGCT1800.019.55539129
CAGAGCG2600.019.46137414
AATGCCG1252.2853783E-819.35983818
CTGTAGG5800.019.3506011
Produced by FastQC (version 0.11.5)