##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062712_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	815147
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248647176521537	32.0	32.0	32.0	32.0	32.0
2	31.359941213057276	32.0	32.0	32.0	32.0	32.0
3	31.481683671779447	32.0	32.0	32.0	32.0	32.0
4	31.596427392850615	32.0	32.0	32.0	32.0	32.0
5	31.52478264656559	32.0	32.0	32.0	32.0	32.0
6	35.12236197888234	36.0	36.0	36.0	36.0	36.0
7	35.13572521275304	36.0	36.0	36.0	36.0	36.0
8	35.06768227080514	36.0	36.0	36.0	36.0	36.0
9	35.21308058546495	36.0	36.0	36.0	36.0	36.0
10	35.071379763404636	36.0	36.0	36.0	36.0	36.0
11	35.21062949382136	36.0	36.0	36.0	36.0	36.0
12	35.125493929315816	36.0	36.0	36.0	36.0	36.0
13	35.172854712094875	36.0	36.0	36.0	36.0	36.0
14	35.12903562179582	36.0	36.0	36.0	36.0	36.0
15	35.08359841844477	36.0	36.0	36.0	36.0	36.0
16	35.10566560387267	36.0	36.0	36.0	36.0	36.0
17	35.068923764670664	36.0	36.0	36.0	36.0	36.0
18	35.09720455328916	36.0	36.0	36.0	36.0	36.0
19	35.06445340533671	36.0	36.0	36.0	36.0	36.0
20	35.05837720067669	36.0	36.0	36.0	36.0	36.0
21	35.03588432515853	36.0	36.0	36.0	36.0	36.0
22	35.01033923942553	36.0	36.0	36.0	36.0	36.0
23	34.95994096770276	36.0	36.0	36.0	32.0	36.0
24	34.933475802523965	36.0	36.0	36.0	32.0	36.0
25	34.89666035696629	36.0	36.0	36.0	32.0	36.0
26	34.85682582405382	36.0	36.0	36.0	32.0	36.0
27	34.812849706862686	36.0	36.0	36.0	32.0	36.0
28	34.793415175422346	36.0	36.0	36.0	32.0	36.0
29	34.766713243132834	36.0	36.0	36.0	32.0	36.0
30	34.742868464215654	36.0	36.0	36.0	32.0	36.0
31	34.73797732188182	36.0	36.0	36.0	32.0	36.0
32	34.68573643772228	36.0	36.0	36.0	32.0	36.0
33	34.68146972263899	36.0	36.0	36.0	32.0	36.0
34	34.6528159951518	36.0	36.0	36.0	32.0	36.0
35	34.60533008156811	36.0	36.0	36.0	32.0	36.0
36	34.584095874731794	36.0	36.0	36.0	32.0	36.0
37	34.54644131671956	36.0	36.0	36.0	32.0	36.0
38	34.48024098720844	36.0	36.0	36.0	32.0	36.0
39	34.408970406564706	36.0	36.0	36.0	32.0	36.0
40	34.389968925850184	36.0	36.0	36.0	32.0	36.0
41	34.3755089572801	36.0	36.0	36.0	32.0	36.0
42	34.29054636771036	36.0	36.0	36.0	32.0	36.0
43	34.30817508989176	36.0	36.0	36.0	32.0	36.0
44	34.27729967723613	36.0	36.0	36.0	32.0	36.0
45	34.174016465741765	36.0	36.0	36.0	32.0	36.0
46	34.22162628335748	36.0	36.0	36.0	32.0	36.0
47	34.17025027387698	36.0	36.0	36.0	32.0	36.0
48	34.163504251380424	36.0	36.0	36.0	32.0	36.0
49	34.16804576352486	36.0	36.0	36.0	32.0	36.0
50	33.629535531628036	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	8.0
21	24.0
22	62.0
23	198.0
24	546.0
25	1378.0
26	2808.0
27	5471.0
28	9185.0
29	13883.0
30	20317.0
31	28963.0
32	43694.0
33	68391.0
34	149010.0
35	471207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.74572444821794	17.58304183470525	11.613403497492763	26.057830219584044
2	15.555869074492099	20.3563892433016	37.53557758366866	26.552164098537638
3	19.33464435074582	23.89531652728417	28.35698766964815	28.413051452321863
4	12.218562341669273	15.507547736905705	35.94415717449043	36.329732746934596
5	13.907430193572448	37.061658817366684	33.766056919794835	15.264854069266034
6	34.868280618591605	34.97979493143526	16.60115660829647	13.550767841676665
7	30.288033937437053	30.38323149076179	20.395707768046744	18.933026803754416
8	28.08119271738717	33.88345905707805	19.385460536565795	18.649887688968985
9	27.133878919998477	14.35029510014758	18.28835780540197	40.22746817445197
10	15.553145628947906	27.25753759751309	31.956444665808743	25.232872107730266
11	37.87660385182059	20.766070414293374	21.904760736407052	19.452564997478984
12	24.237100792863128	23.618194018992895	29.20221751414162	22.942487674002358
13	30.20154646953249	19.365341466017785	25.148592830495602	25.28451923395412
14	23.19557086022521	19.8759242198033	24.814910684821264	32.113594235150224
15	25.142704322042526	27.88049272094481	22.44454067793907	24.532262279073592
16	25.674632918970442	25.836689578689487	24.607586116369195	23.881091385970873
17	23.819016692694692	26.372175816141137	25.826630043415484	23.982177447748686
18	25.013034458815405	24.585626886929596	27.358378304772025	23.04296034948298
19	25.365486225183925	25.97580559089342	24.851836539912433	23.806871644010222
20	25.25924771850967	24.513738012898287	26.11136396257362	24.115650306018424
21	25.96525534658166	24.93881471685475	23.95518845067209	25.1407414858915
22	24.997577124126078	25.672792760078856	24.870360806087735	24.45926930970733
23	23.602614007044128	25.10577846695136	25.37039331556149	25.921214210443022
24	24.208639668673257	25.9085784527208	25.29580554182252	24.586976336783426
25	24.987149557073753	24.96617174570967	25.009231463772792	25.037447233443782
26	23.547286563037094	25.81730657169811	25.955563843085972	24.679843022178822
27	25.033153529363418	25.625807369713684	25.257162205099203	24.083876895823696
28	24.10227848473956	25.07032473897346	25.48656868025031	25.340828096036667
29	23.67401217203768	25.549747468861444	25.793875215145246	24.98236514395563
30	24.367384042387446	25.335552973880787	25.958998806350266	24.3380641773815
31	24.473377194542824	25.177176631944914	25.026038248315945	25.32340792519631
32	24.143498043911098	25.698923016339386	25.16662638763315	24.990952552116365
33	23.675852330929267	25.222935249715693	25.827120752453236	25.2740916669018
34	24.166806723204527	25.759157550723984	26.298937492255998	23.775098233815495
35	25.351991726645622	25.316047289629967	25.66813102422017	23.663829959504238
36	23.827604100855428	26.001322460856752	25.679540009348006	24.49153342893981
37	25.200485311238342	25.61243554843482	25.19655963893629	23.990519501390548
38	24.426023772399333	25.463628032735198	25.083451205733443	25.02689698913202
39	24.808163435552117	25.035116365514444	25.404374916426114	24.752345282507328
40	25.64396360411067	25.120009029046297	25.214715873333276	24.02131149350976
41	23.90599486963701	25.270411349118625	26.320037980879523	24.503555800364843
42	25.759402905242858	25.968812987105395	25.34561250915479	22.926171598496957
43	24.383945472411725	24.643407876125412	25.679540009348006	25.29310664211486
44	23.92513252210951	25.56949850763114	25.993225761733775	24.51214320852558
45	24.34113110886748	25.76970779503574	25.499817824269734	24.389343271827045
46	23.816101655408968	24.891123798681463	26.160712314113056	25.132062231796514
47	24.775807033341266	24.667850912597252	26.381777006818414	24.17456504724307
48	24.553362767697116	26.322368848808864	24.807918081033236	24.316350302460783
49	23.933719930270243	25.453323142942313	26.208892383827703	24.404064542959738
50	23.605190229492347	26.738244758307395	24.649664416356803	25.00690059584345
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	8.5
2	8.0
3	27.5
4	47.0
5	61.5
6	76.0
7	79.0
8	82.0
9	106.0
10	130.0
11	199.5
12	269.0
13	485.5
14	702.0
15	933.0
16	1164.0
17	1424.5
18	1685.0
19	1914.0
20	2143.0
21	2731.0
22	3319.0
23	3811.0
24	4303.0
25	5068.0
26	5833.0
27	7529.5
28	9226.0
29	10686.0
30	12146.0
31	13901.0
32	15656.0
33	18378.5
34	21101.0
35	24640.0
36	28179.0
37	31586.5
38	34994.0
39	36343.5
40	37693.0
41	39481.0
42	41269.0
43	42684.5
44	44100.0
45	49899.5
46	55699.0
47	60652.5
48	65606.0
49	65163.5
50	64721.0
51	60419.5
52	56118.0
53	54238.0
54	52358.0
55	52730.5
56	53103.0
57	51584.0
58	50065.0
59	45663.0
60	41261.0
61	36495.5
62	31730.0
63	27657.0
64	23584.0
65	20141.0
66	16698.0
67	14730.0
68	12762.0
69	11753.5
70	10745.0
71	8268.0
72	5791.0
73	5170.5
74	4550.0
75	3253.5
76	1957.0
77	1759.5
78	1562.0
79	1325.0
80	1088.0
81	896.5
82	705.0
83	584.0
84	463.0
85	358.5
86	254.0
87	190.5
88	127.0
89	84.5
90	42.0
91	29.0
92	16.0
93	11.0
94	6.0
95	3.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013003789500544075
2	0.003312286004855566
3	4.907090377563801E-4
4	2.4535451887819007E-4
5	0.0
6	6.133862971954752E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.2267725943909504E-4
47	1.2267725943909504E-4
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	815147.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.920317896602214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.66219173018499	48.93224488605965
2	11.526240013909238	13.813119315719566
3	3.030238752797056	5.44718608110609
4	1.2612738789146882	3.0230372711699474
5	0.6377109814508852	1.9105922367345625
6	0.3663064637703671	1.316951985402036
7	0.2629377365342855	1.1028718932103199
8	0.18084915681549377	0.8669231174173481
9	0.13280184139020929	0.7161775698019939
>10	0.778620263740718	8.625804838160445
>50	0.0812362063379759	3.3827863524867774
>100	0.07466969740823017	8.637111543391306
>500	0.004513003683734119	1.8193161493200452
>1k	4.1027306215764715E-4	0.40587676001991685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1693	0.2076926002303879	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1609	0.19738771043750392	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	978	0.11997835973143495	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	875	0.10734260200920816	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	865	0.10611582941481719	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	821	0.10071802999949701	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	817	0.10022732096174064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	2.4535451887819007E-4
6	0.0	0.0	0.0	0.0	4.907090377563801E-4
7	0.0	0.0	0.0	0.0	4.907090377563801E-4
8	0.0	0.0	0.0	0.0	4.907090377563801E-4
9	0.0	0.0	0.0	1.2267725943909504E-4	4.907090377563801E-4
10	0.0	0.0	0.0	1.2267725943909504E-4	4.907090377563801E-4
11	0.0	0.0	0.0	1.2267725943909504E-4	4.907090377563801E-4
12	0.0	0.0	0.0	1.2267725943909504E-4	4.907090377563801E-4
13	0.0	0.0	0.0	1.2267725943909504E-4	7.360635566345702E-4
14	0.0	0.0	0.0	1.2267725943909504E-4	7.360635566345702E-4
15	0.0	0.0	0.0	1.2267725943909504E-4	0.0011040953349518553
16	0.0	0.0	0.0	3.680317783172851E-4	0.0011040953349518553
17	0.0	0.0	0.0	4.907090377563801E-4	0.0011040953349518553
18	0.0	0.0	0.0	6.133862971954752E-4	0.0011040953349518553
19	0.0	0.0	0.0	7.360635566345702E-4	0.0011040953349518553
20	0.0	0.0	0.0	8.587408160736652E-4	0.0011040953349518553
21	0.0	0.0	0.0	0.0011040953349518553	0.0011040953349518553
22	0.0	0.0	0.0	0.002698899707660091	0.0011040953349518553
23	0.0	0.0	0.0	0.0046617358586856115	0.0011040953349518553
24	0.0	0.0	0.0	0.006747249269150227	0.0011040953349518553
25	0.0	0.0	0.0	0.008587408160736653	0.0011040953349518553
26	0.0	0.0	0.0	0.011899694165592218	0.0011040953349518553
27	0.0	0.0	0.0	0.015457334689325974	0.0013494498538300455
28	0.0	0.0	0.0	0.02318600203398896	0.0013494498538300455
29	0.0	0.0	0.0	0.03189608745416471	0.0013494498538300455
30	0.0	0.0	0.0	0.05054303088890715	0.0013494498538300455
31	0.0	0.0	0.0	0.08943172213110027	0.0013494498538300455
32	0.0	0.0	0.0	0.13003789500544075	0.0013494498538300455
33	0.0	0.0	0.0	0.17223887225248943	0.0014721271132691403
34	0.0	0.0	0.0	0.22462206203298302	0.0014721271132691403
35	0.0	0.0	0.0	0.2815443104127231	0.0014721271132691403
36	0.0	0.0	0.0	0.3657009103879423	0.0014721271132691403
37	0.0	0.0	0.0	0.4790546981096661	0.0014721271132691403
38	0.0	0.0	0.0	0.6205015782429427	0.0014721271132691403
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATAC	50	8.33863E-5	26.39978	3
GCCCGTA	50	8.33863E-5	26.39978	7
TAACGGC	75	2.063045E-6	23.466469	36
TTAACGG	85	2.3508983E-7	23.293922	35
GTATAGT	60	2.865877E-4	22.006565	1
GTATTAG	220	0.0	21.006268	1
TGTAGGA	560	0.0	20.82253	2
AGCGAAA	245	0.0	20.652885	17
CGTCGTA	185	0.0	20.216047	10
CGTCGTG	55	0.0044825985	19.999834	29
GCGAAAG	255	0.0	19.84297	18
TATTAGG	100	1.1219472E-6	19.80105	2
ACCGTCG	190	0.0	19.684046	8
GTAGGAC	560	0.0	19.642693	3
ATACACT	370	0.0	19.621456	4
CCTAATA	90	1.0008454E-5	19.556591	2
AAACGCT	180	0.0	19.555391	29
CAGAGCG	260	0.0	19.461374	14
AATGCCG	125	2.2853783E-8	19.359838	18
CTGTAGG	580	0.0	19.350601	1
>>END_MODULE