FastQCFastQC Report
Thu 2 Feb 2017
SRR4062711_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062711_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671492
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG56040.335269328240877No Hit
TCCATGTACTCTGCGTTGATACCAC48920.29267265413175775No Hit
GTATCAACGCAGAGTACTTTTTTTT46600.2787928389726065No Hit
TATCAACGCAGAGTACTTTTTTTTT28830.17248063406824563No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28560.17086531075230993No Hit
GAGTACATGGAAGCAGTGGTATCAA26090.15608809375097218No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23770.14220827859182095No Hit
CATGTACTCTGCGTTGATACCACTG20840.12467902927444463No Hit
GCGCAAGACGGACCAGAGCGAAAGC19920.11917496464236742No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19070.1140896875366439No Hit
GAACTACGACGGTATCTGATCGTCT16860.10086796706176278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAT3150.012.9699729
CGACGCT1051.9901563E-812.66834515
AGCGTTC1457.2759576E-1212.4499248
CGCGCCA853.93797E-612.29574610
CGGCGTC1650.012.09214914
GCGTTAT1650.012.0809261
TCGCCAG2450.012.02416217
CGACCAT2150.011.93181410
GAGCGTT1209.993528E-911.87657457
AACCGCG1603.6379788E-1211.8765737
TAACGAA1603.6379788E-1211.87621713
CCAACGA1052.71124E-711.764873519
CTCGACG1052.7165152E-711.7631113
TCGACGC1052.7165152E-711.7631114
AAGACGG4050.011.72748855
CGAACGA1552.5465852E-1111.6484516
GATAACG1552.5465852E-1111.64600711
GCACCGT907.4503805E-611.6126496
CAAGACG4050.011.4905294
GACCATA2400.011.480000511