##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062711_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1671492
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.107956843347143	32.0	32.0	32.0	32.0	32.0
2	30.89877307220136	32.0	32.0	32.0	32.0	32.0
3	30.90233515924695	32.0	32.0	32.0	32.0	32.0
4	30.878259662624767	32.0	32.0	32.0	32.0	32.0
5	30.853802471085714	32.0	32.0	32.0	32.0	32.0
6	34.38876285378572	36.0	36.0	36.0	32.0	36.0
7	34.29587338736889	36.0	36.0	36.0	32.0	36.0
8	34.29114946407162	36.0	36.0	36.0	32.0	36.0
9	34.35648630086175	36.0	36.0	36.0	32.0	36.0
10	34.170211403943306	36.0	36.0	36.0	32.0	36.0
11	34.38120493547083	36.0	36.0	36.0	32.0	36.0
12	34.22753324574691	36.0	36.0	36.0	32.0	36.0
13	34.3158220320528	36.0	36.0	36.0	32.0	36.0
14	34.241584165523975	36.0	36.0	36.0	32.0	36.0
15	34.21425887769729	36.0	36.0	36.0	32.0	36.0
16	34.20716401873297	36.0	36.0	36.0	32.0	36.0
17	34.14800908409971	36.0	36.0	36.0	32.0	36.0
18	34.156325606105206	36.0	36.0	36.0	32.0	36.0
19	34.1423369061892	36.0	36.0	36.0	32.0	36.0
20	34.110337351300515	36.0	36.0	36.0	32.0	36.0
21	34.06986751955738	36.0	36.0	36.0	32.0	36.0
22	34.03878211801193	36.0	36.0	36.0	32.0	36.0
23	33.99907208649518	36.0	36.0	36.0	32.0	36.0
24	33.98746329626466	36.0	36.0	36.0	32.0	36.0
25	33.44549659824875	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	11.0
4	57.0
5	171.0
6	274.0
7	71.0
8	257.0
9	294.0
10	187.0
11	55.0
12	116.0
13	78.0
14	446.0
15	707.0
16	1049.0
17	1509.0
18	2056.0
19	2582.0
20	3414.0
21	4218.0
22	5200.0
23	6943.0
24	8831.0
25	12142.0
26	16230.0
27	21589.0
28	29742.0
29	40244.0
30	53438.0
31	75713.0
32	111793.0
33	168813.0
34	401474.0
35	701787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.38820342163237	17.566888401463235	11.167033432533628	25.87787474437077
2	16.60839349111364	20.775404172933726	36.92598828074987	25.690214055202766
3	19.267545838214406	24.57536157834467	28.45026570189934	27.706826881541584
4	12.660390274024733	15.787536888865485	36.216728217917755	35.33534461919202
5	14.314569461118698	36.65478488915595	33.75533991738614	15.275305732339223
6	33.013853184376934	35.73702591972994	17.400784684584927	13.848336211308196
7	29.136256719323693	30.97351543281754	21.370906667927866	18.519321179930902
8	27.821392964818102	32.38997985072169	20.46591837895613	19.322708805504078
9	27.44859328422114	14.482926706580802	18.966440268324554	39.10203974087351
10	15.726422315739539	26.843443146302693	31.98346157025239	25.446672967705382
11	36.95854129967458	21.642415490245522	22.31517524876598	19.08386796131392
12	24.146887761489797	24.28203188227126	29.133022909204836	22.438057447034108
13	29.534949329656424	19.87904286089929	25.39363181087704	25.19237599856725
14	23.425723927989967	20.094415232947213	25.36728016800904	31.11258067105378
15	24.991315285098228	27.123802108289414	23.286595591758505	24.598287014853856
16	25.36999126754726	26.023128422157193	24.419663231582494	24.187217078713058
17	23.495759158540384	25.759458034837195	26.18869348539665	24.55608932122577
18	24.442671296021736	25.01007879128785	27.16661065674183	23.380639255948584
19	24.91023430732754	25.758231067735508	25.892175738283136	23.439358886653817
20	24.995747229709494	24.579335130278526	26.173884396525903	24.251033243486074
21	26.181098470848358	24.832974612380696	24.53669287036865	24.449234046402296
22	25.028430163654686	25.24227053053262	25.38523021542215	24.344069090390544
23	23.92956142437181	24.873067860692462	25.628460117697173	25.568910597238553
24	23.871884867469273	25.814003502043658	25.709828785648693	24.60428284483838
25	24.329796614718116	25.01383777100466	25.72789071635325	24.928474897923973
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	242.0
1	242.0
2	706.0
3	1170.0
4	1170.0
5	1170.0
6	2973.5
7	4777.0
8	4777.0
9	4777.0
10	5192.0
11	5607.0
12	5607.0
13	5607.0
14	5839.0
15	6071.0
16	6071.0
17	6071.0
18	9889.5
19	13708.0
20	13708.0
21	13708.0
22	21858.5
23	30009.0
24	30009.0
25	30009.0
26	43189.5
27	56370.0
28	56370.0
29	56370.0
30	69660.5
31	82951.0
32	82951.0
33	82951.0
34	97869.0
35	112787.0
36	112787.0
37	112787.0
38	125289.5
39	137792.0
40	137792.0
41	137792.0
42	156649.0
43	175506.0
44	175506.0
45	175506.0
46	202897.0
47	230288.0
48	230288.0
49	230288.0
50	222355.5
51	214423.0
52	214423.0
53	214423.0
54	194592.0
55	174761.0
56	174761.0
57	174761.0
58	158960.0
59	143159.0
60	143159.0
61	143159.0
62	128143.5
63	113128.0
64	113128.0
65	113128.0
66	94863.5
67	76599.0
68	76599.0
69	76599.0
70	58955.5
71	41312.0
72	41312.0
73	41312.0
74	32815.0
75	24318.0
76	24318.0
77	24318.0
78	19842.5
79	15367.0
80	15367.0
81	15367.0
82	10924.0
83	6481.0
84	6481.0
85	6481.0
86	4681.5
87	2882.0
88	2882.0
89	2882.0
90	1907.5
91	933.0
92	933.0
93	933.0
94	546.0
95	159.0
96	159.0
97	159.0
98	425.5
99	692.0
100	692.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007538175474366614
2	0.0032306466318714057
3	0.004187875263537008
4	0.01531565810664963
5	0.03505849863475266
6	0.045288879635678785
7	0.06718548458502943
8	0.08716763227104886
9	0.09416736664010358
10	0.11522639653674682
11	0.11672206627372432
12	0.12940534564329353
13	0.11815790922122271
14	0.12312353274798803
15	0.11319228569445741
16	0.11115817485216802
17	0.12037150043194941
18	0.12832846343266974
19	0.12838829022214884
20	0.11917496464236742
21	0.12773019553787873
22	0.1489687058029593
23	0.13718282827557654
24	0.13048222785391736
25	0.12850794380110703
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1671492.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.63787525853722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.91950240793213	40.75235420806627
2	13.229858516612738	13.663235675378898
3	3.657928327575827	5.666629400520907
4	1.3919658884216377	2.8751264364182187
5	0.7070865641135233	1.8256223897340902
6	0.40148315642383825	1.243904228989077
7	0.2708572683918037	0.9790545686658868
8	0.19906459994166242	0.8223418384142543
9	0.15264950012631648	0.7094246261220714
>10	0.8488607737608816	8.264229738070451
>50	0.10003120142123413	3.6409869095234324
>100	0.10396586334709736	11.230635250071936
>500	0.012326866560572426	4.171093954532077
>1k	0.004302774176803583	3.8188388848907704
>5k	1.1629119396766441E-4	0.33652189060175297
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	5604	0.335269328240877	No Hit
TCCATGTACTCTGCGTTGATACCAC	4892	0.29267265413175775	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4660	0.2787928389726065	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2883	0.17248063406824563	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2856	0.17086531075230993	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2609	0.15608809375097218	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2377	0.14220827859182095	No Hit
CATGTACTCTGCGTTGATACCACTG	2084	0.12467902927444463	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1992	0.11917496464236742	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1907	0.1140896875366439	No Hit
GAACTACGACGGTATCTGATCGTCT	1686	0.10086796706176278	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.982678947910011E-5
7	0.0	0.0	0.0	0.0	5.982678947910011E-5
8	0.0	0.0	0.0	0.0	5.982678947910011E-5
9	0.0	0.0	0.0	5.982678947910011E-5	5.982678947910011E-5
10	0.0	0.0	0.0	5.982678947910011E-5	5.982678947910011E-5
11	0.0	0.0	0.0	5.982678947910011E-5	5.982678947910011E-5
12	0.0	0.0	0.0	5.982678947910011E-5	1.1965357895820022E-4
13	0.0	0.0	0.0	5.982678947910011E-5	1.1965357895820022E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGTAT	315	0.0	12.969972	9
CGACGCT	105	1.9901563E-8	12.668345	15
AGCGTTC	145	7.2759576E-12	12.449924	8
CGCGCCA	85	3.93797E-6	12.295746	10
CGGCGTC	165	0.0	12.092149	14
GCGTTAT	165	0.0	12.080926	1
TCGCCAG	245	0.0	12.024162	17
CGACCAT	215	0.0	11.931814	10
GAGCGTT	120	9.993528E-9	11.8765745	7
AACCGCG	160	3.6379788E-12	11.876573	7
TAACGAA	160	3.6379788E-12	11.876217	13
CCAACGA	105	2.71124E-7	11.7648735	19
CTCGACG	105	2.7165152E-7	11.76311	13
TCGACGC	105	2.7165152E-7	11.76311	14
AAGACGG	405	0.0	11.7274885	5
CGAACGA	155	2.5465852E-11	11.64845	16
GATAACG	155	2.5465852E-11	11.646007	11
GCACCGT	90	7.4503805E-6	11.612649	6
CAAGACG	405	0.0	11.490529	4
GACCATA	240	0.0	11.4800005	11
>>END_MODULE