FastQCFastQC Report
Thu 2 Feb 2017
SRR4062711_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062711_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671492
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30460.18223240075333894No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC27090.16207077269888218No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17760.10625237811488178No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC17460.10445757443050879No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17010.10176536890394929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG3250.018.9538084
TATAGCG750.001291168317.5999645
CGCAATA5450.017.35776336
TCTAGCG5700.016.59645728
CTAGCGG5700.016.59645729
CGTTAGG800.001988011916.5009541
ATCGGCT800.00198880716.49996635
CGAATGC5950.016.26887543
ATACGAA5700.016.21049340
TAGCGGC5850.016.17090830
TAACGGC2500.015.83996936
GTATTAG4800.015.5842331
TTAACGG2403.6379788E-1215.58330135
TTAGCGG850.0029806215.529383
CGGTCCA6100.015.50816410
CGTACTA2001.2769306E-915.3999683
GTATAGT1452.2853546E-615.173291
GACGGCC6850.015.0948611
CGAGCCG5700.015.052615
TAGTGCG2051.8117134E-915.024368
Produced by FastQC (version 0.11.5)