##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062711_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1671492
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.223343575679692	32.0	32.0	32.0	32.0	32.0
2	31.325521749431047	32.0	32.0	32.0	32.0	32.0
3	31.431104067503764	32.0	32.0	32.0	32.0	32.0
4	31.546018168199428	32.0	32.0	32.0	32.0	32.0
5	31.457351874851927	32.0	32.0	32.0	32.0	32.0
6	35.03895920530879	36.0	36.0	36.0	36.0	36.0
7	35.044293960126645	36.0	36.0	36.0	36.0	36.0
8	34.99451866954792	36.0	36.0	36.0	36.0	36.0
9	35.122685002381104	36.0	36.0	36.0	36.0	36.0
10	34.98781806912626	36.0	36.0	36.0	36.0	36.0
11	35.13212088361775	36.0	36.0	36.0	36.0	36.0
12	35.022202319843586	36.0	36.0	36.0	36.0	36.0
13	35.076333598964275	36.0	36.0	36.0	36.0	36.0
14	35.035300797132145	36.0	36.0	36.0	36.0	36.0
15	34.992997872559364	36.0	36.0	36.0	36.0	36.0
16	35.00887231287975	36.0	36.0	36.0	36.0	36.0
17	34.98535081232815	36.0	36.0	36.0	36.0	36.0
18	35.001478918235925	36.0	36.0	36.0	36.0	36.0
19	34.97929634123286	36.0	36.0	36.0	36.0	36.0
20	34.95966118892582	36.0	36.0	36.0	36.0	36.0
21	34.948728441416414	36.0	36.0	36.0	32.0	36.0
22	34.935312283875724	36.0	36.0	36.0	32.0	36.0
23	34.89258279429396	36.0	36.0	36.0	32.0	36.0
24	34.87319711969905	36.0	36.0	36.0	32.0	36.0
25	34.840289992413965	36.0	36.0	36.0	32.0	36.0
26	34.80356651422801	36.0	36.0	36.0	32.0	36.0
27	34.78370342185305	36.0	36.0	36.0	32.0	36.0
28	34.76233389091901	36.0	36.0	36.0	32.0	36.0
29	34.740304470497016	36.0	36.0	36.0	32.0	36.0
30	34.72323887879811	36.0	36.0	36.0	32.0	36.0
31	34.714595104254165	36.0	36.0	36.0	32.0	36.0
32	34.67492695149004	36.0	36.0	36.0	32.0	36.0
33	34.64179667028021	36.0	36.0	36.0	32.0	36.0
34	34.62386717974121	36.0	36.0	36.0	32.0	36.0
35	34.59150148490092	36.0	36.0	36.0	32.0	36.0
36	34.56130690425081	36.0	36.0	36.0	32.0	36.0
37	34.54295084870284	36.0	36.0	36.0	32.0	36.0
38	34.49959377609944	36.0	36.0	36.0	32.0	36.0
39	34.47120476795581	36.0	36.0	36.0	32.0	36.0
40	34.444827734742375	36.0	36.0	36.0	32.0	36.0
41	34.413635841511656	36.0	36.0	36.0	32.0	36.0
42	34.36290930498022	36.0	36.0	36.0	32.0	36.0
43	34.355429759759545	36.0	36.0	36.0	32.0	36.0
44	34.3250335628289	36.0	36.0	36.0	32.0	36.0
45	34.24815613834826	36.0	36.0	36.0	32.0	36.0
46	34.27330133796632	36.0	36.0	36.0	32.0	36.0
47	34.22454968375559	36.0	36.0	36.0	32.0	36.0
48	34.188236019077564	36.0	36.0	36.0	32.0	36.0
49	34.17529608278113	36.0	36.0	36.0	32.0	36.0
50	33.58843476367222	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	7.0
21	39.0
22	139.0
23	403.0
24	1140.0
25	2793.0
26	5737.0
27	11049.0
28	18971.0
29	29612.0
30	43354.0
31	62730.0
32	92129.0
33	147830.0
34	311184.0
35	944371.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5507245509627	18.284964116507364	11.716196696827868	26.44811463570207
2	16.03457167711882	21.260348947545392	36.50637240296609	26.1987069723697
3	18.658580434261072	24.75334777559808	28.84827425705197	27.73979753308888
4	12.445811690914551	16.298670763619917	36.384331068282954	34.87118647718258
5	14.179077243012376	36.745157467195455	33.627581602294	15.448183687498169
6	33.181432024313786	35.987986694426496	17.07869672386149	13.751884557398233
7	29.1601156332187	30.880076003953356	21.333814340720743	18.625994022107196
8	27.75400660009141	32.61882198658444	20.287443792731285	19.339727620592857
9	27.550715169441432	14.217238251813349	18.71896485295772	39.5130817257875
10	15.626936892309388	26.84422061248274	32.037485073216025	25.49135742199185
11	37.1660767745224	21.43324646483501	22.2436601551189	19.157016605523687
12	24.077470906232275	24.214474254139414	29.16352576021901	22.544529079409294
13	29.78398939390676	19.725012144838264	25.312834282186213	25.178164179068762
14	23.48811720307366	19.85848571216614	25.371823496612606	31.281573588147594
15	25.10625237811488	27.080895391662057	23.08859390293223	24.724258327290826
16	25.470238565305724	25.977450086509542	24.330418572149913	24.221892776034824
17	23.678486047196156	25.674846185324252	25.99252643745827	24.65414133002132
18	24.696618350551482	24.792281386928565	27.057862077712606	23.453238184807347
19	25.077714999533352	25.732579037171583	25.69177716674683	23.497928796548234
20	25.185522874174687	24.46951585768882	26.059472614885383	24.285488653251107
21	26.344607093542777	24.756804100767457	24.491711596585567	24.406877209104202
22	25.14878922543452	25.17343786269991	25.25994740028669	24.41782551157888
23	24.05934338902011	24.598203281858364	25.683760376956634	25.65869295216489
24	24.018661172174323	25.734672974803352	25.615916797687337	24.630749055334995
25	24.68088390491848	24.64636384738904	25.66880367958686	25.00394856810562
26	23.715877790620596	25.443137029671693	26.307753791223647	24.53323138848406
27	25.113252112483934	25.242298497390355	25.50822857662495	24.136220813500753
28	23.944116992483362	25.02823824463414	26.163451575000057	24.864193187882442
29	23.80184888710206	25.472751290463847	26.1195387115224	24.60586111091169
30	24.2795059743032	25.456119442988662	26.231594288216755	24.03278029449139
31	24.17433047839894	25.27412634939324	25.430633230670562	25.120909941537263
32	24.09308569828632	25.557226717208337	25.50786961588808	24.841817968617256
33	23.93364730432452	24.8084944468774	26.042362153094363	25.21549609570372
34	24.230627487298772	25.408018704247464	26.36961469154504	23.991739116908725
35	25.331560067293175	25.012324318632693	25.727733067223774	23.928382546850358
36	23.927186011060776	25.764765849911335	25.611788749213275	24.69625938981461
37	25.080407205059913	25.468383934831873	25.375831891507705	24.075376968600505
38	24.007593216120686	25.21065012575591	25.767577709016855	25.014178949106547
39	24.350101585888538	25.038468625635062	25.638351843741997	24.973077944734403
40	24.933173476151847	25.184386165174587	25.76907337875383	24.113366979919736
41	23.496971567916567	25.417351683406203	26.55555635324608	24.530120395431148
42	25.16619882117294	25.61950640505608	25.73413453369804	23.48016024007294
43	24.298590720147033	24.426799530000743	26.044216783568213	25.230392966284015
44	23.771038090520324	25.168472239173145	26.16249434636839	24.897995323938137
45	23.91438307811225	25.460486798620636	25.988817176510565	24.63631294675655
46	23.627601720620525	24.89473463795775	26.219959437388198	25.257704204033526
47	23.9205452374286	24.718455128711355	26.69190160646895	24.669098027391097
48	24.209149669875778	25.99539812335327	25.38313075982416	24.412321446946798
49	23.328977943059254	25.334970194293483	26.555855487193476	24.780196375453787
50	23.25610891347371	26.160400408736628	25.53790266420659	25.045588013583075
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	24.5
2	33.0
3	106.5
4	180.0
5	219.0
6	258.0
7	273.5
8	289.0
9	389.0
10	489.0
11	752.5
12	1016.0
13	1693.0
14	2370.0
15	3362.0
16	4354.0
17	5223.0
18	6092.0
19	6839.5
20	7587.0
21	8896.0
22	10205.0
23	10943.0
24	11681.0
25	13273.5
26	14866.0
27	18350.5
28	21835.0
29	24687.0
30	27539.0
31	29867.0
32	32195.0
33	36508.0
34	40821.0
35	46825.5
36	52830.0
37	61157.0
38	69484.0
39	73735.5
40	77987.0
41	84208.0
42	90429.0
43	92134.0
44	93839.0
45	103547.5
46	113256.0
47	120572.5
48	127889.0
49	126737.0
50	125585.0
51	117499.0
52	109413.0
53	105756.5
54	102100.0
55	102823.5
56	103547.0
57	101404.0
58	99261.0
59	90090.0
60	80919.0
61	71794.0
62	62669.0
63	55492.0
64	48315.0
65	41751.0
66	35187.0
67	32108.5
68	29030.0
69	26878.5
70	24727.0
71	19783.0
72	14839.0
73	12892.5
74	10946.0
75	8368.0
76	5790.0
77	5044.5
78	4299.0
79	3730.5
80	3162.0
81	2433.5
82	1705.0
83	1431.0
84	1157.0
85	945.5
86	734.0
87	555.5
88	377.0
89	245.5
90	114.0
91	76.0
92	38.0
93	30.0
94	22.0
95	15.5
96	9.0
97	7.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014956697369775026
2	0.003948568105620607
3	5.38441105311901E-4
4	3.5896073687460064E-4
5	5.9826789479100115E-5
6	7.179214737492013E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.1965357895820023E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1671492.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.35827497999094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.31099711211826	45.26189259162019
2	12.519598690952474	14.11165971327668
3	3.383323760992854	5.7203487260511725
4	1.315541260358027	2.965665443951262
5	0.6118942444502853	1.7242652043701483
6	0.38228776514196494	1.2927047393614233
7	0.24469232712993486	0.9653306220517935
8	0.1735970322335226	0.7826903422661775
9	0.137742726033944	0.6986648187283115
>10	0.7354802776852739	7.773615711354682
>50	0.08893153820676503	3.473607129045464
>100	0.0851259811606407	9.997971561563244
>500	0.00801035906139128	2.9980494773218958
>1k	0.002776924474615644	2.2335339190376104
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3046	0.18223240075333894	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2709	0.16207077269888218	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1776	0.10625237811488178	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1746	0.10445757443050879	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1701	0.10176536890394929	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	5.982678947910011E-5
6	0.0	0.0	0.0	0.0	5.982678947910011E-5
7	0.0	0.0	0.0	0.0	5.982678947910011E-5
8	0.0	0.0	0.0	0.0	5.982678947910011E-5
9	0.0	0.0	0.0	0.0	5.982678947910011E-5
10	0.0	0.0	0.0	0.0	5.982678947910011E-5
11	0.0	0.0	0.0	0.0	5.982678947910011E-5
12	0.0	0.0	0.0	0.0	2.3930715791640043E-4
13	0.0	0.0	0.0	0.0	2.3930715791640043E-4
14	0.0	0.0	0.0	0.0	2.3930715791640043E-4
15	0.0	0.0	0.0	0.0	2.3930715791640043E-4
16	0.0	0.0	0.0	0.0	2.3930715791640043E-4
17	0.0	0.0	0.0	0.0	2.3930715791640043E-4
18	0.0	0.0	0.0	5.982678947910011E-5	2.3930715791640043E-4
19	0.0	0.0	0.0	5.982678947910011E-5	2.3930715791640043E-4
20	0.0	0.0	0.0	2.991339473955005E-4	2.3930715791640043E-4
21	0.0	0.0	0.0	5.38441105311901E-4	2.3930715791640043E-4
22	0.0	0.0	0.0	0.0010170554211447018	2.3930715791640043E-4
23	0.0	0.0	0.0	0.0017948036843730033	2.3930715791640043E-4
24	0.0	0.0	0.0	0.0029315126844759055	2.3930715791640043E-4
25	0.0	0.0	0.0	0.0035297805792669063	2.3930715791640043E-4
26	5.982678947910011E-5	0.0	0.0	0.0045468360004116085	2.3930715791640043E-4
27	5.982678947910011E-5	0.0	0.0	0.006580946842701012	2.3930715791640043E-4
28	5.982678947910011E-5	0.0	0.0	0.015674618843524227	2.3930715791640043E-4
29	5.982678947910011E-5	0.0	0.0	0.03541745937162726	2.3930715791640043E-4
30	5.982678947910011E-5	0.0	0.0	0.06898028826940243	2.3930715791640043E-4
31	5.982678947910011E-5	0.0	0.0	0.14490048411838047	2.3930715791640043E-4
32	5.982678947910011E-5	0.0	0.0	0.23236725033682482	2.3930715791640043E-4
33	5.982678947910011E-5	0.0	0.0	0.3185776539762081	2.3930715791640043E-4
34	5.982678947910011E-5	0.0	0.0	0.4223771337224468	2.3930715791640043E-4
35	5.982678947910011E-5	0.0	0.0	0.5357488997853415	2.3930715791640043E-4
36	5.982678947910011E-5	0.0	0.0	0.7012896262740115	2.3930715791640043E-4
37	5.982678947910011E-5	0.0	0.0	0.9417933199799939	2.3930715791640043E-4
38	5.982678947910011E-5	0.0	0.0	1.2442177407968449	2.3930715791640043E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	325	0.0	18.953808	4
TATAGCG	75	0.0012911683	17.599964	5
CGCAATA	545	0.0	17.357763	36
TCTAGCG	570	0.0	16.596457	28
CTAGCGG	570	0.0	16.596457	29
CGTTAGG	80	0.0019880119	16.500954	1
ATCGGCT	80	0.001988807	16.499966	35
CGAATGC	595	0.0	16.268875	43
ATACGAA	570	0.0	16.210493	40
TAGCGGC	585	0.0	16.170908	30
TAACGGC	250	0.0	15.839969	36
GTATTAG	480	0.0	15.584233	1
TTAACGG	240	3.6379788E-12	15.583301	35
TTAGCGG	85	0.00298062	15.52938	3
CGGTCCA	610	0.0	15.508164	10
CGTACTA	200	1.2769306E-9	15.399968	3
GTATAGT	145	2.2853546E-6	15.17329	1
GACGGCC	685	0.0	15.09486	11
CGAGCCG	570	0.0	15.0526	15
TAGTGCG	205	1.8117134E-9	15.02436	8
>>END_MODULE