FastQCFastQC Report
Thu 2 Feb 2017
SRR4062710_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062710_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677461
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG38040.5615083377493317No Hit
TCCATGTACTCTGCGTTGATACCAC32120.47412323366215914No Hit
GTATCAACGCAGAGTACTTTTTTTT25350.3741912818597676No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18680.27573542978857823No Hit
GAGTACATGGAAGCAGTGGTATCAA16950.2501989044387795No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15130.22333388933089876No Hit
TATCAACGCAGAGTACTTTTTTTTT15050.22215300954593697No Hit
GGGTAGGCACACGCTGAGCCAGTCA14990.22126734970721562No Hit
CATGTACTCTGCGTTGATACCACTG14390.2124107513200022No Hit
GTATTAGAGGCACTGCCTGCCCAGT13420.19809258392734047No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC13240.19543560441117647No Hit
GAATAATTGCAATCCCCGATCCCCA12560.1853981262390012No Hit
GTACATGGGGAATAATTGCAATCCC11870.17521303809370578No Hit
CCATAGGGTCTTCTCGTCTTATTAT11780.17388454833562375No Hit
GTGCATGGCCGTTCTTAGTTGGTGG11190.16517555992153055No Hit
CTCTAATACTTGTAATGCTAGAGGT10760.1588283310773609No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10600.1564665715074373No Hit
GTCCTAAAGTGTGTATTTCTCATTT10060.14849563295894525No Hit
CATCTAAGGGCATCACAGACCTGTT9790.14451016368469918No Hit
GCGTTGATACCACTGCTTCCATGTA9600.14170557419541494No Hit
GTCCTACAGTGGACATTTCTAAATT9430.13919620465237115No Hit
GCGCAAGACGGACCAGAGCGAAAGC9420.1390485946792509No Hit
CCATTGGGATGTCCTGATCCAACAT9410.1389009847061307No Hit
CATGGAAGCAGTGGTATCAACGCAG9260.13668683510932733No Hit
GATTAAGAGGGACGGCCGGGGGCAT9200.13580117527060598No Hit
GCTTCCATGTACTCTGCGTTGATAC9170.1353583453512453No Hit
GAATAACGCCGCCGCATCGCCAGTC9140.13491551543188465No Hit
TCGTAGTTCCGACCATAAACGATGC9130.1347679054587644No Hit
AGTCAGTGTAGCGCGCGTGCAGCCC9130.1347679054587644No Hit
GAATAGGACCGCGGTTCTATTTTGT9030.13329180572756216No Hit
CTGTAGGACGTGGAATATGGCAAGA9020.13314419575444195No Hit
GTCTTGCGCCGGTCCAAGAATTTCA9000.1328489758082015No Hit
GAACTACGACGGTATCTGATCGTCT8880.1310776561307588No Hit
GTTATTGCTCAATCTCGGGTGGCTG8810.13004438631891727No Hit
GGTATCAACGCAGAGTACTTTTTTT8710.12856828658771502No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG8160.1204497380661027No Hit
GGATTGACAGATTGATAGCTCTTTC8150.12030212809298249No Hit
CTTCTACACCATTGGGATGTCCTGA8070.11912124830802068No Hit
GTCAGGATACCGCGGCCGTTAAACT8040.11867841838866001No Hit
GATATATTTTGATCAACGGACCAAG7950.117349928630578No Hit
ACCCCCGAGCGGTCGGCGTCCCCCA7850.11587382889937577No Hit
GGAGTGGAGCCTGCGGCTTAATTTG7850.11587382889937577No Hit
GCCCCGGACATCTAAGGGCATCACA7740.11425011919505329No Hit
GTACATGGGGTGGTATCAACGCAAA7690.11351206932945217No Hit
CTTTAGGACGTGAAATATGGCGAGG7630.11262640949073083No Hit
GTATCTGATCGTCTTCGAACCTCCG7540.11129791973264881No Hit
TCACAGACCTGTTATTGCTCAATCT7520.11100269978640838No Hit
ATCTATAACTTTATAGATGCAACAC7510.11085508981328815No Hit
CTGTTAGTATGAGTAACAAGAATTC7470.11026464992080726No Hit
GTGTATATCAATGAGTTACAATGAA7280.10746006043152301No Hit
CCCCGATCCCCATCACGAATGGGGT7260.10716484048528255No Hit
GCCATGCACCACCACCCACGGAATC7250.10701723051216232No Hit
TTGTAGAACAGTGTATATCAATGAG7180.10598396070032076No Hit
GTTATATAATTTAAGCTCCATAGGG7120.10509830086159941No Hit
GTATCAACGCAGAGTACATGGGGAA7090.10465547094223875No Hit
ATCAGATACCGTCGTAGTTCCGACC6980.10303176123791628No Hit
GTATCCTGACCGTGCAAAGGTAGCA6900.1018508814529545No Hit
CCCATGTACTCTGCGTTGATACCAC6890.10170327147983427No Hit
GATATACACTGTTCTACAAATCCCG6880.10155566150671404No Hit
ACACTGACTGGCTCAGCGTGTGCCT6810.10052239169487248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGT250.00602619819.0013316
CAATACA651.000808E-817.5370984
TAAAGCA350.002169624216.2844474
CCAATAC350.002169624216.2844473
CTAAGTC350.002169624216.2844474
TCGCCAG1700.016.20701617
TCGGCAA651.9086838E-716.0768613
GTGTTCA755.8234946E-815.2010649
CTCGGCA755.8289515E-815.19994112
ACAATAC755.8342266E-815.1988173
CGTTTAT755.8500518E-815.195451
ACATTCG456.7442E-414.78099818
CGCGGGT456.751763E-414.77881317
CAATTAG653.3758042E-614.611011
AGAATTT1450.014.41480217
ATCGCCA1850.014.37832416
CGCCAGT1800.014.25310518
GTACAGC602.5712874E-514.2457331
GACAATT551.9539648E-413.82119218
CCGCATC1950.013.64097312
Produced by FastQC (version 0.11.5)