##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062710_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	677461
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.065273130113763	32.0	32.0	32.0	32.0	32.0
2	30.787363405421125	32.0	32.0	32.0	32.0	32.0
3	30.787818044138334	32.0	32.0	32.0	32.0	32.0
4	30.829795368294263	32.0	32.0	32.0	32.0	32.0
5	30.674117034043288	32.0	32.0	32.0	32.0	32.0
6	34.283406720091634	36.0	36.0	36.0	32.0	36.0
7	34.11889688114888	36.0	36.0	36.0	32.0	36.0
8	34.11290686843966	36.0	36.0	36.0	32.0	36.0
9	34.25071849154416	36.0	36.0	36.0	32.0	36.0
10	33.9755233142572	36.0	36.0	36.0	32.0	36.0
11	34.30117748475558	36.0	36.0	36.0	32.0	36.0
12	34.054859246510134	36.0	36.0	36.0	32.0	36.0
13	34.205264952521254	36.0	36.0	36.0	32.0	36.0
14	34.10939522717913	36.0	36.0	36.0	32.0	36.0
15	34.061875148532536	36.0	36.0	36.0	32.0	36.0
16	34.05502752187949	36.0	36.0	36.0	32.0	36.0
17	33.95460993326553	36.0	36.0	36.0	32.0	36.0
18	34.02003510165161	36.0	36.0	36.0	32.0	36.0
19	33.96952149275014	36.0	36.0	36.0	32.0	36.0
20	33.93510917971662	36.0	36.0	36.0	32.0	36.0
21	33.89368982125908	36.0	36.0	36.0	32.0	36.0
22	33.8484030815058	36.0	36.0	36.0	32.0	36.0
23	33.813134630628184	36.0	36.0	36.0	32.0	36.0
24	33.791365111792416	36.0	36.0	36.0	32.0	36.0
25	33.34454677095803	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	27.0
5	70.0
6	123.0
7	35.0
8	77.0
9	122.0
10	98.0
11	27.0
12	48.0
13	34.0
14	161.0
15	207.0
16	296.0
17	407.0
18	591.0
19	765.0
20	1144.0
21	1742.0
22	2424.0
23	3519.0
24	4729.0
25	6197.0
26	8396.0
27	10719.0
28	14126.0
29	18631.0
30	24394.0
31	33029.0
32	47239.0
33	68271.0
34	151256.0
35	278553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.49024296990444	18.85242150606207	11.310006214856903	24.347329309176583
2	15.98096944808152	21.591380453090103	38.18022930545571	24.24742079337267
3	19.24924419565885	26.598112851035687	28.672736720281538	25.479906233023925
4	11.230743111071742	16.555868045559567	38.48838496800053	33.72500387536816
5	12.53470089306809	39.20447833482966	34.39449156546803	13.86632920663422
6	30.73078882661764	37.95701068456535	18.53709323704672	12.7751072517703
7	28.13988629364246	32.25166061798475	21.61307047046781	17.995382617904983
8	27.35650999691221	32.94039083472333	20.752623515385036	18.950475652979424
9	27.486299477392844	14.725790079477068	19.859515158858095	37.92839528427199
10	16.343978281527328	26.11197072676193	32.219626133695115	25.324424858015632
11	35.884756633735066	22.619355506129416	23.512277305273734	17.983610554861784
12	24.118502577685284	25.80936953128695	29.27402095256113	20.79810693846663
13	28.30659367943155	20.646333100814605	27.17690734325676	23.870165876497083
14	22.520665126665808	22.48741174923111	25.15847081757493	29.83345230652816
15	24.316000721165924	29.03210075042634	24.278169066119684	22.373729462288047
16	24.630594285460955	26.82271924574587	26.07378508781652	22.47290138097666
17	22.14640620243025	27.03025842474115	26.840940059529615	23.982395313298987
18	23.401503719392423	24.734661663136706	29.959547081019643	21.90428753645123
19	23.599106076551063	27.09941942273356	26.34945785198236	22.952016648733018
20	23.520492087959834	25.706334509264334	27.70891495609841	23.064258446677417
21	25.617194994154328	25.35351161522328	24.882754013263984	24.146539377358405
22	24.432108803311404	26.955429078276293	25.87094389829256	22.741518220119744
23	22.844785535429743	26.15258631648343	27.043909880240406	23.958718267846425
24	22.75596824425495	27.71059761521509	26.549688931458498	22.983745209071465
25	23.335486417096174	26.50820561407969	26.91660175390105	23.239706214923086
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	182.0
1	182.0
2	405.0
3	628.0
4	628.0
5	628.0
6	2095.5
7	3563.0
8	3563.0
9	3563.0
10	3549.5
11	3536.0
12	3536.0
13	3536.0
14	3424.0
15	3312.0
16	3312.0
17	3312.0
18	5397.0
19	7482.0
20	7482.0
21	7482.0
22	11983.0
23	16484.0
24	16484.0
25	16484.0
26	25175.0
27	33866.0
28	33866.0
29	33866.0
30	40828.0
31	47790.0
32	47790.0
33	47790.0
34	51877.5
35	55965.0
36	55965.0
37	55965.0
38	57240.5
39	58516.0
40	58516.0
41	58516.0
42	63260.5
43	68005.0
44	68005.0
45	68005.0
46	82698.5
47	97392.0
48	97392.0
49	97392.0
50	88213.5
51	79035.0
52	79035.0
53	79035.0
54	66379.5
55	53724.0
56	53724.0
57	53724.0
58	49686.5
59	45649.0
60	45649.0
61	45649.0
62	41795.0
63	37941.0
64	37941.0
65	37941.0
66	33310.0
67	28679.0
68	28679.0
69	28679.0
70	21839.5
71	15000.0
72	15000.0
73	15000.0
74	12832.0
75	10664.0
76	10664.0
77	10664.0
78	8222.0
79	5780.0
80	5780.0
81	5780.0
82	4046.0
83	2312.0
84	2312.0
85	2312.0
86	1733.5
87	1155.0
88	1155.0
89	1155.0
90	781.0
91	407.0
92	407.0
93	407.0
94	247.0
95	87.0
96	87.0
97	87.0
98	197.0
99	307.0
100	307.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007675718602251642
2	0.003542639354885373
3	0.005461569005448284
4	0.015646657150743735
5	0.03616444341445486
6	0.046644751505990754
7	0.06701492779658165
8	0.08886120381837478
9	0.09771780220558823
10	0.11838319844241955
11	0.11897363833490045
12	0.13063482621139816
13	0.12000690814674203
14	0.12369715747474763
15	0.11498816906065441
16	0.1130692394100915
17	0.12148300787794426
18	0.12871589656083524
19	0.13181570599635994
20	0.12310671758226674
21	0.1316680960232397
22	0.14923368282454635
23	0.13860576475989025
24	0.13609639521684644
25	0.1346202954856442
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	677461.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.412026635618034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.25255500967134	25.81164425652687
2	12.56302151784617	8.395120191562377
3	3.859604629930158	3.868716380945434
4	1.6397631137032094	2.1915123532462237
5	0.8665767076605225	1.4477042019079782
6	0.5560694729591991	1.1147644825060106
7	0.38846534052788423	0.908559001331247
8	0.2876397895311995	0.7688502647424003
9	0.23159642422693952	0.6964295305485958
>10	1.6896000215260103	11.705114694258826
>50	0.3006713137639251	7.2890737164991535
>100	0.3166126499844706	22.2659556450822
>500	0.03985334055748581	9.154470570616681
>1k	0.007970668111497162	4.382084710226022
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3804	0.5615083377493317	No Hit
TCCATGTACTCTGCGTTGATACCAC	3212	0.47412323366215914	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2535	0.3741912818597676	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1868	0.27573542978857823	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1695	0.2501989044387795	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1513	0.22333388933089876	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1505	0.22215300954593697	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1499	0.22126734970721562	No Hit
CATGTACTCTGCGTTGATACCACTG	1439	0.2124107513200022	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1342	0.19809258392734047	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1324	0.19543560441117647	No Hit
GAATAATTGCAATCCCCGATCCCCA	1256	0.1853981262390012	No Hit
GTACATGGGGAATAATTGCAATCCC	1187	0.17521303809370578	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1178	0.17388454833562375	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	1119	0.16517555992153055	No Hit
CTCTAATACTTGTAATGCTAGAGGT	1076	0.1588283310773609	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1060	0.1564665715074373	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1006	0.14849563295894525	No Hit
CATCTAAGGGCATCACAGACCTGTT	979	0.14451016368469918	No Hit
GCGTTGATACCACTGCTTCCATGTA	960	0.14170557419541494	No Hit
GTCCTACAGTGGACATTTCTAAATT	943	0.13919620465237115	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	942	0.1390485946792509	No Hit
CCATTGGGATGTCCTGATCCAACAT	941	0.1389009847061307	No Hit
CATGGAAGCAGTGGTATCAACGCAG	926	0.13668683510932733	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	920	0.13580117527060598	No Hit
GCTTCCATGTACTCTGCGTTGATAC	917	0.1353583453512453	No Hit
GAATAACGCCGCCGCATCGCCAGTC	914	0.13491551543188465	No Hit
TCGTAGTTCCGACCATAAACGATGC	913	0.1347679054587644	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCC	913	0.1347679054587644	No Hit
GAATAGGACCGCGGTTCTATTTTGT	903	0.13329180572756216	No Hit
CTGTAGGACGTGGAATATGGCAAGA	902	0.13314419575444195	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	900	0.1328489758082015	No Hit
GAACTACGACGGTATCTGATCGTCT	888	0.1310776561307588	No Hit
GTTATTGCTCAATCTCGGGTGGCTG	881	0.13004438631891727	No Hit
GGTATCAACGCAGAGTACTTTTTTT	871	0.12856828658771502	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	816	0.1204497380661027	No Hit
GGATTGACAGATTGATAGCTCTTTC	815	0.12030212809298249	No Hit
CTTCTACACCATTGGGATGTCCTGA	807	0.11912124830802068	No Hit
GTCAGGATACCGCGGCCGTTAAACT	804	0.11867841838866001	No Hit
GATATATTTTGATCAACGGACCAAG	795	0.117349928630578	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCA	785	0.11587382889937577	No Hit
GGAGTGGAGCCTGCGGCTTAATTTG	785	0.11587382889937577	No Hit
GCCCCGGACATCTAAGGGCATCACA	774	0.11425011919505329	No Hit
GTACATGGGGTGGTATCAACGCAAA	769	0.11351206932945217	No Hit
CTTTAGGACGTGAAATATGGCGAGG	763	0.11262640949073083	No Hit
GTATCTGATCGTCTTCGAACCTCCG	754	0.11129791973264881	No Hit
TCACAGACCTGTTATTGCTCAATCT	752	0.11100269978640838	No Hit
ATCTATAACTTTATAGATGCAACAC	751	0.11085508981328815	No Hit
CTGTTAGTATGAGTAACAAGAATTC	747	0.11026464992080726	No Hit
GTGTATATCAATGAGTTACAATGAA	728	0.10746006043152301	No Hit
CCCCGATCCCCATCACGAATGGGGT	726	0.10716484048528255	No Hit
GCCATGCACCACCACCCACGGAATC	725	0.10701723051216232	No Hit
TTGTAGAACAGTGTATATCAATGAG	718	0.10598396070032076	No Hit
GTTATATAATTTAAGCTCCATAGGG	712	0.10509830086159941	No Hit
GTATCAACGCAGAGTACATGGGGAA	709	0.10465547094223875	No Hit
ATCAGATACCGTCGTAGTTCCGACC	698	0.10303176123791628	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	690	0.1018508814529545	No Hit
CCCATGTACTCTGCGTTGATACCAC	689	0.10170327147983427	No Hit
GATATACACTGTTCTACAAATCCCG	688	0.10155566150671404	No Hit
ACACTGACTGGCTCAGCGTGTGCCT	681	0.10052239169487248	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.476099731202239E-4	0.0
5	0.0	0.0	0.0	1.476099731202239E-4	0.0
6	0.0	0.0	0.0	1.476099731202239E-4	0.0
7	0.0	0.0	0.0	2.952199462404478E-4	0.0
8	0.0	0.0	0.0	2.952199462404478E-4	0.0
9	0.0	0.0	0.0	4.428299193606717E-4	0.0
10	0.0	0.0	0.0	5.904398924808956E-4	0.0
11	0.0	0.0	0.0	5.904398924808956E-4	0.0
12	0.0	0.0	0.0	5.904398924808956E-4	0.0
13	0.0	0.0	0.0	5.904398924808956E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGCGT	25	0.006026198	19.001331	6
CAATACA	65	1.000808E-8	17.537098	4
TAAAGCA	35	0.0021696242	16.284447	4
CCAATAC	35	0.0021696242	16.284447	3
CTAAGTC	35	0.0021696242	16.284447	4
TCGCCAG	170	0.0	16.207016	17
TCGGCAA	65	1.9086838E-7	16.07686	13
GTGTTCA	75	5.8234946E-8	15.201064	9
CTCGGCA	75	5.8289515E-8	15.199941	12
ACAATAC	75	5.8342266E-8	15.198817	3
CGTTTAT	75	5.8500518E-8	15.19545	1
ACATTCG	45	6.7442E-4	14.780998	18
CGCGGGT	45	6.751763E-4	14.778813	17
CAATTAG	65	3.3758042E-6	14.61101	1
AGAATTT	145	0.0	14.414802	17
ATCGCCA	185	0.0	14.378324	16
CGCCAGT	180	0.0	14.253105	18
GTACAGC	60	2.5712874E-5	14.245733	1
GACAATT	55	1.9539648E-4	13.821192	18
CCGCATC	195	0.0	13.640973	12
>>END_MODULE