##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062710_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	677461
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.216390316195323	32.0	32.0	32.0	32.0	32.0
2	31.43047644071024	32.0	32.0	32.0	32.0	32.0
3	31.51536841235141	32.0	32.0	32.0	32.0	32.0
4	31.621696304289102	32.0	32.0	32.0	32.0	32.0
5	31.56712784942602	32.0	32.0	32.0	32.0	32.0
6	35.14593607602504	36.0	36.0	36.0	36.0	36.0
7	35.15353208524181	36.0	36.0	36.0	36.0	36.0
8	35.11162856607244	36.0	36.0	36.0	36.0	36.0
9	35.208138918697905	36.0	36.0	36.0	36.0	36.0
10	35.12087928308788	36.0	36.0	36.0	36.0	36.0
11	35.23084871306245	36.0	36.0	36.0	36.0	36.0
12	35.131464394260334	36.0	36.0	36.0	36.0	36.0
13	35.19564816277247	36.0	36.0	36.0	36.0	36.0
14	35.150832298833436	36.0	36.0	36.0	36.0	36.0
15	35.112188007870564	36.0	36.0	36.0	36.0	36.0
16	35.115160872729206	36.0	36.0	36.0	36.0	36.0
17	35.090926267342326	36.0	36.0	36.0	36.0	36.0
18	35.119791397585985	36.0	36.0	36.0	36.0	36.0
19	35.074116443603394	36.0	36.0	36.0	36.0	36.0
20	35.046545852824	36.0	36.0	36.0	36.0	36.0
21	35.03526697477788	36.0	36.0	36.0	36.0	36.0
22	35.0098116349133	36.0	36.0	36.0	36.0	36.0
23	34.96792287674124	36.0	36.0	36.0	36.0	36.0
24	34.935084086021185	36.0	36.0	36.0	32.0	36.0
25	34.914031951654785	36.0	36.0	36.0	32.0	36.0
26	34.869149663227844	36.0	36.0	36.0	32.0	36.0
27	34.841852150898724	36.0	36.0	36.0	32.0	36.0
28	34.805668518187765	36.0	36.0	36.0	32.0	36.0
29	34.780970417485285	36.0	36.0	36.0	32.0	36.0
30	34.74566949241358	36.0	36.0	36.0	32.0	36.0
31	34.747671083649095	36.0	36.0	36.0	32.0	36.0
32	34.69196603199298	36.0	36.0	36.0	32.0	36.0
33	34.67875051109953	36.0	36.0	36.0	32.0	36.0
34	34.64121624713452	36.0	36.0	36.0	32.0	36.0
35	34.57928943511139	36.0	36.0	36.0	32.0	36.0
36	34.52154441362676	36.0	36.0	36.0	32.0	36.0
37	34.484836765511226	36.0	36.0	36.0	32.0	36.0
38	34.41578629618532	36.0	36.0	36.0	32.0	36.0
39	34.32212924434027	36.0	36.0	36.0	32.0	36.0
40	34.29188691304739	36.0	36.0	36.0	32.0	36.0
41	34.26695706468712	36.0	36.0	36.0	32.0	36.0
42	34.16710482227021	36.0	36.0	36.0	32.0	36.0
43	34.198231337302076	36.0	36.0	36.0	32.0	36.0
44	34.13236924339556	36.0	36.0	36.0	32.0	36.0
45	34.02815660237268	36.0	36.0	36.0	32.0	36.0
46	34.04824927191381	36.0	36.0	36.0	32.0	36.0
47	33.98880821183803	36.0	36.0	36.0	32.0	36.0
48	33.935980078558025	36.0	36.0	36.0	32.0	36.0
49	33.9473371898899	36.0	36.0	36.0	32.0	36.0
50	33.39925988359477	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	12.0
22	63.0
23	183.0
24	519.0
25	1251.0
26	2676.0
27	5191.0
28	8652.0
29	12969.0
30	17706.0
31	24742.0
32	36458.0
33	54932.0
34	116279.0
35	395822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.89509348726314	19.506174662109792	11.882367704265794	24.716364146361265
2	15.36833217208517	22.09122096387534	37.86653125332137	24.673915610718126
3	18.60144835547001	26.799004513932967	29.089626220370857	25.50992091022617
4	10.980000265698736	16.97563985421996	38.590527249619534	33.45383263046177
5	12.34786947145297	39.27827579742598	34.24329961429514	14.130555116825914
6	30.850768608533663	38.16339905942508	18.28290462286961	12.702927709171638
7	27.96721877716946	32.3344074419044	21.812030508029245	17.88634327289689
8	27.25278650726758	32.98595786325707	20.729606575138643	19.031649054336707
9	27.50593761116876	14.486442762018772	19.899300476337384	38.10831915047508
10	16.280199155375733	26.178185902952343	32.22207625235992	25.319538689312004
11	36.084291199050575	22.584768717313615	23.52932493530993	17.80161514832588
12	23.9188676543742	25.80502789090442	29.434757129930727	20.84134732479065
13	28.42200510435287	20.403093314596706	27.41294332810302	23.7619582529474
14	22.562479611372463	22.37501494550978	25.21399755853104	29.848507884586716
15	24.34915072601965	29.047723780409502	24.20833081166296	22.39479468190789
16	24.810579501993473	26.696739738523696	26.07589219158003	22.416788567902802
17	22.286744181583884	26.90516502056945	26.67326975279758	24.134821045049087
18	23.49817923098156	24.561413867366536	29.986670819427243	21.95373608222466
19	23.73435518797392	26.94561015320439	26.48211483760689	22.8379198212148
20	23.626009467703675	25.40160983436685	27.74787035711281	23.224510340816668
21	25.79971393187209	25.1180510760029	25.001882027157286	24.080352964967723
22	24.38501994948787	26.868705357208754	25.953523523863364	22.792751169440013
23	22.85076188887626	25.79853305208713	27.15684002473943	24.193865034297175
24	22.92353360562453	27.40511999952765	26.61909689266246	23.052249502185365
25	23.49758879108908	25.96223251227746	27.185328749551633	23.354849947081824
26	21.35473481130279	27.133074819067076	28.81833788218067	22.693852487449462
27	24.121683757441385	26.513703371854614	27.068274040867298	22.2963388298367
28	22.16467073381346	26.163572515613442	27.71111547380587	23.960641276767223
29	22.084518518409176	25.967841691256027	27.85621607738305	24.091423712951745
30	22.538271575780747	26.160472706177924	28.484886952902084	22.81636876513925
31	22.788470480219523	26.137740770317407	26.917711868284666	24.1560768811784
32	22.21264397507753	26.751355428578176	27.16953448242777	23.86646611391652
33	21.88125958542263	26.22320694475401	27.943010741577744	23.95252272824561
34	22.81695920503173	26.712386395674436	28.791325257099672	21.679329142194163
35	24.197998113544543	26.163867735559688	27.507856540819326	22.130277610076448
36	21.81586836733037	26.801838039385295	27.688826367864717	23.693467225419617
37	24.52628269376392	26.44388385456875	26.155453967091834	22.874379484575496
38	22.359663508305275	26.71622425497556	26.738808580862955	24.185303655856206
39	23.4028231883459	26.48344332736497	26.127112852252747	23.98662063203638
40	24.232686457227796	26.403291111960687	27.142817077293007	22.221205353518506
41	22.50889719112982	26.595331686990097	28.34155766900235	22.55421345287773
42	24.303244024379264	27.870681854748835	26.74530341968025	21.080770701191657
43	22.163194634082256	26.415690349702786	27.09720559559886	24.323909420616094
44	21.936465715369593	26.99874974352767	27.555386952164035	23.5093975889387
45	22.66669225239534	27.15713524468567	27.304597607832775	22.871574895086212
46	22.963717415050336	26.10013875357955	27.124405868981192	23.811737962388925
47	24.03666041292414	25.43895515756627	27.362017887376545	23.162366542133054
48	22.945379881646325	28.25033470561405	25.466705832512872	23.33757958022676
49	21.803321519615153	26.952695431914165	27.7704546830002	23.473528365470482
50	21.093169938933755	28.65832867131835	25.708195748537555	24.54030564121034
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	33.5
2	32.0
3	117.0
4	202.0
5	226.5
6	251.0
7	214.5
8	178.0
9	253.5
10	329.0
11	487.0
12	645.0
13	1086.0
14	1527.0
15	2103.0
16	2679.0
17	3411.5
18	4144.0
19	4460.0
20	4776.0
21	5752.5
22	6729.0
23	6551.0
24	6373.0
25	8011.0
26	9649.0
27	12949.5
28	16250.0
29	17865.0
30	19480.0
31	18950.0
32	18420.0
33	21567.0
34	24714.0
35	28615.0
36	32516.0
37	36919.0
38	41322.0
39	39556.0
40	37790.0
41	38983.5
42	40177.0
43	36835.5
44	33494.0
45	37053.0
46	40612.0
47	42867.5
48	45123.0
49	45154.0
50	45185.0
51	39511.0
52	33837.0
53	31953.0
54	30069.0
55	30878.0
56	31687.0
57	31974.5
58	32262.0
59	29887.5
60	27513.0
61	24298.0
62	21083.0
63	18940.0
64	16797.0
65	15505.0
66	14213.0
67	13372.0
68	12531.0
69	11293.0
70	10055.0
71	7341.0
72	4627.0
73	4143.5
74	3660.0
75	2742.0
76	1824.0
77	1737.5
78	1651.0
79	1403.0
80	1155.0
81	953.5
82	752.0
83	647.5
84	543.0
85	436.5
86	330.0
87	254.0
88	178.0
89	109.5
90	41.0
91	27.5
92	14.0
93	8.0
94	2.0
95	2.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014170557419541492
2	0.004280689220486493
3	4.4282991936067163E-4
4	2.952199462404478E-4
5	0.0
6	4.4282991936067163E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.476099731202239E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	677461.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.646153694447285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5502121757329	31.142137846505502
2	12.249372501506812	9.712810097105505
3	3.591259399462314	4.27138866323134
4	1.5092133782912305	2.3933802221380054
5	0.8266939590179847	1.6387617878749063
6	0.5152688997112101	1.2257057995151628
7	0.375704594841665	1.042666947756189
8	0.25976666916787877	0.8238999432419489
9	0.19675386682953766	0.7020480639870595
>10	1.4086564266383033	11.350901643701304
>50	0.2486264718969886	7.132727998729006
>100	0.23889109777438036	19.720995318559122
>500	0.025087309640883206	6.666446387324681
>1k	0.00449324948791938	2.1761292803302936
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1668	0.24621343516453348	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1532	0.226138478820183	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1406	0.20753962220703479	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	1295	0.19115491519068994	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC	1259	0.18584095615836188	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	1162	0.17152278876570018	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1130	0.166799269625853	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	1113	0.1642899000828092	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1041	0.15366198201815306	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1021	0.15070978255574857	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	1020	0.15056217258262838	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	1012	0.14938129279766657	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	979	0.14451016368469918	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	966	0.1425912340341363	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT	943	0.13919620465237115	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	900	0.1328489758082015	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	885	0.13063482621139816	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC	876	0.12930633645331613	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC	873	0.12886350653395545	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	870	0.12842067661459478	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	855	0.12620652701779142	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	836	0.12340193752850717	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG	814	0.12015451811986226	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT	796	0.11749753860369823	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	782	0.11543099898001508	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT	782	0.11543099898001508	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	779	0.11498816906065441	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	770	0.1136596793025724	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	764	0.11277401946385104	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	756	0.11159313967888927	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG	739	0.10908377013584544	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	734	0.10834572027024433	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	732	0.10805050032400389	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	723	0.10672201056592188	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAA	717	0.10583635072720053	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	708	0.10450786096911852	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	701	0.10347459115727695	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	678	0.10007956177551179	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.476099731202239E-4	0.0
5	0.0	0.0	0.0	1.476099731202239E-4	0.0
6	0.0	0.0	0.0	1.476099731202239E-4	0.0
7	0.0	0.0	0.0	1.476099731202239E-4	0.0
8	0.0	0.0	0.0	1.476099731202239E-4	0.0
9	0.0	0.0	0.0	1.476099731202239E-4	0.0
10	0.0	0.0	0.0	1.476099731202239E-4	0.0
11	0.0	0.0	0.0	1.476099731202239E-4	0.0
12	0.0	0.0	0.0	1.476099731202239E-4	0.0
13	0.0	0.0	0.0	1.476099731202239E-4	0.0
14	0.0	0.0	0.0	1.476099731202239E-4	0.0
15	0.0	0.0	0.0	1.476099731202239E-4	0.0
16	0.0	0.0	0.0	1.476099731202239E-4	0.0
17	0.0	0.0	0.0	1.476099731202239E-4	0.0
18	0.0	0.0	0.0	1.476099731202239E-4	0.0
19	0.0	0.0	0.0	1.476099731202239E-4	0.0
20	0.0	0.0	0.0	4.428299193606717E-4	0.0
21	0.0	0.0	0.0	0.0010332698118415674	0.0
22	0.0	0.0	0.0	0.0019189296505629106	0.0
23	0.0	0.0	0.0	0.004428299193606717	0.0
24	0.0	0.0	0.0	0.008118548521612314	0.0
25	0.0	0.0	0.0	0.010480308091535896	0.0
26	0.0	0.0	0.0	0.013727727500180822	0.0
27	0.0	0.0	0.0	0.01889407655938866	0.0
28	0.0	0.0	0.0	0.029374384650924554	0.0
29	0.0	0.0	0.0	0.04590670164038963	0.0
30	0.0	0.0	0.0	0.07956177551180067	0.0
31	0.0	0.0	0.0	0.13166809602323973	0.0
32	0.0	0.0	0.0	0.20429220279838986	0.0
33	0.0	0.0	0.0	0.26938820094440863	0.0
34	0.0	0.0	0.0	0.3529354457304553	0.0
35	0.0	0.0	0.0	0.4502104180166829	0.0
36	0.0	0.0	0.0	0.5839450536636057	0.0
37	0.0	0.0	0.0	0.7643244408165193	0.0
38	0.0	0.0	0.0	0.985001350631254	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTACC	30	0.0057433946	29.333185	15
TGCGTTA	30	0.0057433946	29.333185	13
TACCGTT	30	0.0057433946	29.333185	18
TTACCGT	40	7.0291274E-4	27.49986	17
ATAGCAC	45	0.0013976209	24.444323	3
GTAAGAC	55	1.5926646E-4	23.99988	3
ATCATGC	55	1.5926646E-4	23.99988	7
ATAGTAC	50	0.0025793542	21.999887	3
CCTGCGT	50	0.0025793542	21.999887	11
TTACCGC	155	1.8189894E-12	21.290216	16
CAATTAA	95	6.857463E-7	20.842001	37
TGTAGGA	585	0.0	20.685183	2
CGTCGTA	245	0.0	20.652958	10
TAAGACA	75	5.468296E-5	20.533232	4
CTGTAGG	570	0.0	20.459057	1
GTCTAAA	65	4.9243495E-4	20.310589	1
TGGTATA	130	1.6207196E-9	20.307589	44
GTATTAG	450	0.0	20.047302	1
TATAGGA	55	0.004479829	20.001375	2
AGCGTAT	110	1.263652E-7	19.9999	8
>>END_MODULE