FastQCFastQC Report
Thu 2 Feb 2017
SRR4062709_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062709_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928244
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG23510.25327392366662216No Hit
TCCATGTACTCTGCGTTGATACCAC20460.22041618367584387No Hit
GTATCAACGCAGAGTACTTTTTTTT18120.19520729463373854No Hit
TATCAACGCAGAGTACTTTTTTTTT11640.12539806344021615No Hit
GTCCTAAAGTGTGTATTTCTCATTT9560.10299016206945588No Hit
GAGTACATGGAAGCAGTGGTATCAA9460.10191285911893856No Hit
GTCCTACAGTGGACATTTCTAAATT9340.10062009557831778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC551.9626331E-413.8150063
TAGACTG852.6990529E-713.4101285
GTTTTCG500.001497693413.30124615
GATAACG500.001498911813.29981111
ATACCGT1551.8189894E-1212.2572296
AACGAAC550.003065925312.09139114
ATAACGA550.003065925312.09139112
ACGCGCG550.003065925312.09139113
AAGCTCG951.0384374E-611.999837
CGTCGTA1603.6379788E-1211.87547210
CCTACAC1052.723209E-711.75923
CCGTCAA1701.8189894E-1211.73892818
CCGTCGT1552.5465852E-1111.6456249
TCGCGTA907.448065E-611.6115739
GGTCGCG907.4525396E-611.6109477
TACCGTC1800.011.6109477
TTTAAGA907.4794407E-611.607192
CCAGTAC1157.081326E-811.5625573
CGGTCCA1406.7666406E-1011.53617310
ACCGTCG1657.2759576E-1211.5149888
Produced by FastQC (version 0.11.5)