##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062709_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	928244
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.029359737310447	32.0	32.0	32.0	32.0	32.0
2	30.570415752754663	32.0	32.0	32.0	32.0	32.0
3	30.568092010290396	32.0	32.0	32.0	32.0	32.0
4	30.645077156437317	32.0	32.0	32.0	32.0	32.0
5	30.39079595451196	32.0	32.0	32.0	32.0	32.0
6	34.03861376965539	36.0	36.0	36.0	32.0	36.0
7	33.895849582652836	36.0	36.0	36.0	32.0	36.0
8	33.88706633169727	36.0	36.0	36.0	32.0	36.0
9	34.07519682324906	36.0	36.0	36.0	32.0	36.0
10	33.66522810812674	36.0	36.0	36.0	27.0	36.0
11	34.08308914466455	36.0	36.0	36.0	32.0	36.0
12	33.804287450282466	36.0	36.0	36.0	32.0	36.0
13	33.95652867134073	36.0	36.0	36.0	32.0	36.0
14	33.82597894519113	36.0	36.0	36.0	32.0	36.0
15	33.76440677235727	36.0	36.0	36.0	32.0	36.0
16	33.76605720047746	36.0	36.0	36.0	32.0	36.0
17	33.66411309957296	36.0	36.0	36.0	27.0	36.0
18	33.69155308302558	36.0	36.0	36.0	27.0	36.0
19	33.67437225557073	36.0	36.0	36.0	27.0	36.0
20	33.64792015892373	36.0	36.0	36.0	27.0	36.0
21	33.62788340134706	36.0	36.0	36.0	27.0	36.0
22	33.564715742843475	36.0	36.0	36.0	27.0	36.0
23	33.53200774796282	36.0	36.0	36.0	27.0	36.0
24	33.47919189351076	36.0	36.0	36.0	27.0	36.0
25	32.99066516993377	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	42.0
5	92.0
6	174.0
7	54.0
8	150.0
9	144.0
10	115.0
11	47.0
12	64.0
13	51.0
14	260.0
15	246.0
16	395.0
17	572.0
18	801.0
19	1155.0
20	1922.0
21	2832.0
22	4183.0
23	6154.0
24	8157.0
25	11070.0
26	14474.0
27	18091.0
28	23719.0
29	30004.0
30	37653.0
31	49935.0
32	69508.0
33	95569.0
34	199510.0
35	351097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.75644495393075	17.78406495375837	12.23129864421096	25.228191448099913
2	15.53369503387172	19.98000448175409	39.56516211883543	24.92113836553876
3	19.345417014115583	24.63536717539652	28.2251339698382	27.794081840649692
4	12.469776963689258	16.16668462450167	36.29899795280681	35.06454045900227
5	14.17796002823565	37.167705745739056	33.608828489985505	15.045505736039788
6	33.10810155370048	35.818858895110395	17.492414729705697	13.580624821483426
7	29.431461563257006	30.701851789963015	20.98095169304509	18.885734953734897
8	27.08532819198951	34.68455353984973	19.495484071323204	18.734634196837554
9	27.11447856108374	15.257912950267926	18.801284145845667	38.82632434280267
10	16.102098027308546	27.159697511295867	31.470948939121506	25.26725552227408
11	36.58601831576904	21.584777848490404	22.744237220490362	19.084966615250195
12	24.9307416469248	23.543309125273474	29.458749207088946	22.06720002071278
13	29.593605557383327	20.175286255939508	25.325681863535603	24.905426323141562
14	23.283295830375884	20.733993644273482	25.03176784554678	30.950942679803852
15	24.8627478897151	28.15604351512092	22.770259921520918	24.210948673643067
16	24.837431739666442	26.1790715408203	25.19875446125704	23.784742258256227
17	23.28010881489064	26.52686556377612	25.933496498140933	24.259529123192305
18	24.09364181928609	25.235091625592382	27.73881608968488	22.93245046543665
19	25.331351231226307	25.508920538274495	25.293161963489336	23.866566267009865
20	25.424480220046924	25.00563600571691	25.776339562602814	23.793544211633364
21	26.189489899611	24.738239945458115	24.504365724049997	24.56790443088089
22	25.21549980092771	25.64968240147001	25.00682456498119	24.127993232621098
23	23.591275021523312	25.373987757066015	25.774893138648963	25.259844082761717
24	24.256834533150222	26.03093812267584	25.496819127738696	24.215408216435243
25	24.484881959757146	25.586533358073382	25.478655234462316	24.44992944770716
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	180.0
1	180.0
2	391.5
3	603.0
4	603.0
5	603.0
6	1188.0
7	1773.0
8	1773.0
9	1773.0
10	1893.5
11	2014.0
12	2014.0
13	2014.0
14	2352.0
15	2690.0
16	2690.0
17	2690.0
18	4383.5
19	6077.0
20	6077.0
21	6077.0
22	10294.0
23	14511.0
24	14511.0
25	14511.0
26	22641.5
27	30772.0
28	30772.0
29	30772.0
30	40209.0
31	49646.0
32	49646.0
33	49646.0
34	58219.5
35	66793.0
36	66793.0
37	66793.0
38	73380.5
39	79968.0
40	79968.0
41	79968.0
42	90854.5
43	101741.0
44	101741.0
45	101741.0
46	115569.5
47	129398.0
48	129398.0
49	129398.0
50	125915.5
51	122433.0
52	122433.0
53	122433.0
54	110601.5
55	98770.0
56	98770.0
57	98770.0
58	89858.5
59	80947.0
60	80947.0
61	80947.0
62	70555.5
63	60164.0
64	60164.0
65	60164.0
66	49168.0
67	38172.0
68	38172.0
69	38172.0
70	28827.0
71	19482.0
72	19482.0
73	19482.0
74	15191.0
75	10900.0
76	10900.0
77	10900.0
78	8718.0
79	6536.0
80	6536.0
81	6536.0
82	4554.0
83	2572.0
84	2572.0
85	2572.0
86	1901.0
87	1230.0
88	1230.0
89	1230.0
90	818.5
91	407.0
92	407.0
93	407.0
94	247.5
95	88.0
96	88.0
97	88.0
98	232.5
99	377.0
100	377.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008187502423931639
2	0.0038782906218623554
3	0.005386514752586604
4	0.015513162487449422
5	0.036520570022537176
6	0.04944820542874503
7	0.07056334325888451
8	0.09264805374448959
9	0.1007278258733695
10	0.1222738848837159
11	0.12647536639073348
12	0.13789477766621708
13	0.12809132081650945
14	0.1299227358323889
15	0.12044246986783647
16	0.11796467308164663
17	0.12550579373526788
18	0.1362788232404411
19	0.13811023825632054
20	0.1259367149154748
21	0.1346628688146651
22	0.15567027634975286
23	0.14457405595942446
24	0.13972619268209652
25	0.1370329353058032
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	928244.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.56420699133016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.77915782591896	47.893315264937755
2	11.632293641423932	13.624721052005695
3	3.050699661012694	5.359854192477845
4	1.162263483502011	2.722681569051048
5	0.6122046671428667	1.7926640423806608
6	0.3529439505117004	1.2401929544463004
7	0.2480383568179864	1.0168318769334572
8	0.18639801156545951	0.8733001385673539
9	0.12064163942113726	0.6358753748549608
>10	0.6777157492271808	7.600803007359897
>50	0.08467403470948057	3.515342186589996
>100	0.08374586586415327	9.54867084818595
>500	0.008485263851823544	3.379250719299259
>1k	7.378490305933516E-4	0.7964967729098634
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	2351	0.25327392366662216	No Hit
TCCATGTACTCTGCGTTGATACCAC	2046	0.22041618367584387	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1812	0.19520729463373854	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1164	0.12539806344021615	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	956	0.10299016206945588	No Hit
GAGTACATGGAAGCAGTGGTATCAA	946	0.10191285911893856	No Hit
GTCCTACAGTGGACATTTCTAAATT	934	0.10062009557831778	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1546059010346417E-4
2	0.0	0.0	0.0	0.0	2.1546059010346417E-4
3	0.0	0.0	0.0	0.0	2.1546059010346417E-4
4	0.0	0.0	0.0	0.0	2.1546059010346417E-4
5	0.0	0.0	0.0	0.0	3.2319088515519626E-4
6	0.0	0.0	0.0	0.0	4.3092118020692834E-4
7	0.0	0.0	0.0	0.0	4.3092118020692834E-4
8	0.0	0.0	0.0	0.0	4.3092118020692834E-4
9	0.0	0.0	0.0	0.0	4.3092118020692834E-4
10	0.0	0.0	0.0	1.0773029505173208E-4	4.3092118020692834E-4
11	0.0	0.0	0.0	1.0773029505173208E-4	4.3092118020692834E-4
12	0.0	0.0	0.0	1.0773029505173208E-4	5.386514752586605E-4
13	0.0	0.0	0.0	1.0773029505173208E-4	6.463817703103925E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGGAC	55	1.9626331E-4	13.815006	3
TAGACTG	85	2.6990529E-7	13.410128	5
GTTTTCG	50	0.0014976934	13.301246	15
GATAACG	50	0.0014989118	13.299811	11
ATACCGT	155	1.8189894E-12	12.257229	6
AACGAAC	55	0.0030659253	12.091391	14
ATAACGA	55	0.0030659253	12.091391	12
ACGCGCG	55	0.0030659253	12.091391	13
AAGCTCG	95	1.0384374E-6	11.99983	7
CGTCGTA	160	3.6379788E-12	11.875472	10
CCTACAC	105	2.723209E-7	11.7592	3
CCGTCAA	170	1.8189894E-12	11.738928	18
CCGTCGT	155	2.5465852E-11	11.645624	9
TCGCGTA	90	7.448065E-6	11.611573	9
GGTCGCG	90	7.4525396E-6	11.610947	7
TACCGTC	180	0.0	11.610947	7
TTTAAGA	90	7.4794407E-6	11.60719	2
CCAGTAC	115	7.081326E-8	11.562557	3
CGGTCCA	140	6.7666406E-10	11.536173	10
ACCGTCG	165	7.2759576E-12	11.514988	8
>>END_MODULE