FastQCFastQC Report
Thu 2 Feb 2017
SRR4062709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928244
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10690.11516368541030161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC752.0635416E-623.46649436
TTAACGG856.1113005E-620.7057335
GATATAC4350.019.7293051
CCGGTTT1950.019.17934622
CGCAATA2350.018.72326736
AGTCCGT600.00741115218.3331996
GTAGAAC6750.018.2517173
GTCCTAC7800.018.0560131
AATGCCG1951.8189894E-1218.0511518
GTAGGAC8450.017.9643653
GCATTCG3100.017.74180622
GTATTAG3000.017.6046121
CTATATA1002.4764233E-517.6046121
CTGTAGG7800.017.4917621
ATACACT5350.017.27094
GGACGTG8200.017.1706076
TAGGACG7700.017.142734
ATACGAA2700.017.11098740
TGTAGGA8800.017.000792
TGTAGAA7250.016.9938952
Produced by FastQC (version 0.11.5)