##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062709_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	928244
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238662463748756	32.0	32.0	32.0	32.0	32.0
2	31.366342254838166	32.0	32.0	32.0	32.0	32.0
3	31.475392246004283	32.0	32.0	32.0	32.0	32.0
4	31.58324104438057	32.0	32.0	32.0	32.0	32.0
5	31.52725791925399	32.0	32.0	32.0	32.0	32.0
6	35.09830712614356	36.0	36.0	36.0	36.0	36.0
7	35.114672435264865	36.0	36.0	36.0	36.0	36.0
8	35.056954852387946	36.0	36.0	36.0	36.0	36.0
9	35.17813419747394	36.0	36.0	36.0	36.0	36.0
10	35.06383019981815	36.0	36.0	36.0	36.0	36.0
11	35.195413059497284	36.0	36.0	36.0	36.0	36.0
12	35.1069363227772	36.0	36.0	36.0	36.0	36.0
13	35.14689564381779	36.0	36.0	36.0	36.0	36.0
14	35.10772490853698	36.0	36.0	36.0	36.0	36.0
15	35.06904865531046	36.0	36.0	36.0	36.0	36.0
16	35.079645007131745	36.0	36.0	36.0	36.0	36.0
17	35.05513528770452	36.0	36.0	36.0	36.0	36.0
18	35.0717279077484	36.0	36.0	36.0	36.0	36.0
19	35.04294021830467	36.0	36.0	36.0	36.0	36.0
20	35.03270907218361	36.0	36.0	36.0	36.0	36.0
21	35.021504044195275	36.0	36.0	36.0	36.0	36.0
22	34.99868784500627	36.0	36.0	36.0	36.0	36.0
23	34.95393560313883	36.0	36.0	36.0	32.0	36.0
24	34.93657055687944	36.0	36.0	36.0	32.0	36.0
25	34.89938960014824	36.0	36.0	36.0	32.0	36.0
26	34.846281796596585	36.0	36.0	36.0	32.0	36.0
27	34.82244646881639	36.0	36.0	36.0	32.0	36.0
28	34.79020602341626	36.0	36.0	36.0	32.0	36.0
29	34.77290238342505	36.0	36.0	36.0	32.0	36.0
30	34.754233800595536	36.0	36.0	36.0	32.0	36.0
31	34.74508103472794	36.0	36.0	36.0	32.0	36.0
32	34.7090721836069	36.0	36.0	36.0	32.0	36.0
33	34.68703702905702	36.0	36.0	36.0	32.0	36.0
34	34.66179366632049	36.0	36.0	36.0	32.0	36.0
35	34.62788986516476	36.0	36.0	36.0	32.0	36.0
36	34.60245905171485	36.0	36.0	36.0	32.0	36.0
37	34.57176130413986	36.0	36.0	36.0	32.0	36.0
38	34.529461003787794	36.0	36.0	36.0	32.0	36.0
39	34.48181081698347	36.0	36.0	36.0	32.0	36.0
40	34.46433157661132	36.0	36.0	36.0	32.0	36.0
41	34.44257436622267	36.0	36.0	36.0	32.0	36.0
42	34.382641848479494	36.0	36.0	36.0	32.0	36.0
43	34.3800530894894	36.0	36.0	36.0	32.0	36.0
44	34.34894058027846	36.0	36.0	36.0	32.0	36.0
45	34.28126656353287	36.0	36.0	36.0	32.0	36.0
46	34.28904361353265	36.0	36.0	36.0	32.0	36.0
47	34.24283916728791	36.0	36.0	36.0	32.0	36.0
48	34.200307246801486	36.0	36.0	36.0	32.0	36.0
49	34.19498967943773	36.0	36.0	36.0	32.0	36.0
50	33.6944025493297	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	34.0
22	125.0
23	340.0
24	806.0
25	1871.0
26	3634.0
27	6784.0
28	10940.0
29	16310.0
30	23147.0
31	32455.0
32	46842.0
33	73475.0
34	158388.0
35	553087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.36475988320098	18.286265340645844	12.782488767253176	25.566486008899997
2	15.073103620651947	20.33359082011155	39.26102372002676	25.33228183920974
3	18.85548872650255	24.727898741595645	28.709739625247376	27.70687290665443
4	12.271463399131477	16.556117309799266	36.50778944737531	34.664629843693945
5	13.92080099628977	37.276836693800334	33.59526159070245	15.20710071920745
6	33.254869430319744	35.95600275790744	17.220977333448246	13.56815047832457
7	29.413925648859568	30.825623435217466	20.885349110794145	18.875101805128825
8	27.238527800879943	34.53229969706241	19.40351890235757	18.82565359970008
9	27.1832621595184	14.966108049176723	18.82877777825658	39.02185201304829
10	16.139829613765347	27.177013802405405	31.375586591456557	25.3075699923727
11	36.71814738366205	21.311853348903952	22.81576826782613	19.15423099960786
12	24.967357720599324	23.54219364735996	29.357798165137616	22.132650466903097
13	29.761463580696457	20.086098051805344	25.331701578464283	24.82073678903392
14	23.346663161841068	20.42727989623418	25.171183438837204	31.05487350308755
15	24.87923433924701	28.00944579227014	22.718164620509263	24.393155247973596
16	24.962725317912103	26.01869767000918	25.109561710067613	23.90901530201111
17	23.344077634759827	26.365481489780706	25.951258505306797	24.339182370152677
18	24.295012949181466	25.018098689568692	27.75283222945691	22.934056131792936
19	25.48651001245362	25.12701401786599	25.560197534269008	23.82627843541138
20	25.502885017301484	24.735522125648	26.041428762265095	23.720164094785424
21	26.274772581347143	24.429244896815923	24.640827196297526	24.655155325539404
22	25.206949896794377	25.361327409603508	25.209535423875618	24.222187269726493
23	23.71693218593387	25.028225337303557	25.89814746984629	25.356695006916286
24	24.465550006248357	25.665342302239498	25.658232102766082	24.210875588746063
25	24.73735354066388	25.002370066491135	25.68602651888943	24.574249873955555
26	23.592180504263965	25.875955029065633	26.653336838158932	23.87852762851147
27	24.74123183128574	25.506547847333245	25.673852995548586	24.07836732583243
28	23.771335984934996	25.537143251127937	25.774042169946693	24.91747859399037
29	23.87174061992321	25.139510732091992	25.94027001521152	25.04847863277328
30	24.00058605280508	25.388906365136755	26.288992980293973	24.32151460176419
31	24.696416028544217	24.965634035878498	25.40614321234503	24.931806723232253
32	24.05897587272312	25.662218123682994	25.316619337157043	24.962186666436843
33	23.6007989278681	25.355402243375664	26.178892618751103	24.864906210005127
34	24.274975114301846	25.500299490220247	26.521474957015613	23.7032504384623
35	24.76299335088619	25.41853219627598	26.199684565696092	23.61878988714174
36	23.815613136201257	25.84428232232042	25.852577555039407	24.48752698643891
37	24.811364253364417	25.606198370256095	25.21190549036676	24.37053188601273
38	24.1913764053417	25.6249434415951	25.483816755077328	24.699863397985876
39	24.878264766591542	24.70837409129496	25.72394758274764	24.689413559365857
40	25.472289613506792	25.141880798583134	25.718453337700005	23.667376250210072
41	24.0918336127139	25.0435230392009	26.852206962824432	24.01243638526077
42	25.39687840697058	25.852469824744357	25.70735711730967	23.04329465097539
43	24.043570440530722	25.077565812437246	26.00684733755349	24.872016409478544
44	23.701203562856318	25.700139187541204	25.855917194186013	24.742740055416466
45	24.072549889899637	25.723408931272377	25.783845626796403	24.42019555203158
46	24.073166343654286	24.878668363065195	25.99820520748383	25.049960085796684
47	24.63048508797256	24.911014776287267	26.225539836508503	24.232960299231667
48	24.548610063733243	25.88511210414503	25.018421880453843	24.54785595166788
49	24.11176371729847	25.54425345060135	25.762838219261315	24.581144612838866
50	23.722749621866665	26.609813798958033	24.977807559219343	24.689629019955962
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	13.0
2	16.0
3	63.0
4	110.0
5	135.0
6	160.0
7	142.5
8	125.0
9	179.0
10	233.0
11	342.5
12	452.0
13	830.0
14	1208.0
15	1697.5
16	2187.0
17	2320.5
18	2454.0
19	2623.5
20	2793.0
21	3125.0
22	3457.0
23	3874.5
24	4292.0
25	5211.5
26	6131.0
27	8321.0
28	10511.0
29	12667.0
30	14823.0
31	17229.5
32	19636.0
33	23090.0
34	26544.0
35	30966.5
36	35389.0
37	38949.5
38	42510.0
39	43655.5
40	44801.0
41	46928.0
42	49055.0
43	49645.0
44	50235.0
45	56000.0
46	61765.0
47	66291.0
48	70817.0
49	71431.5
50	72046.0
51	68325.5
52	64605.0
53	62759.5
54	60914.0
55	60830.0
56	60746.0
57	58148.0
58	55550.0
59	50696.0
60	45842.0
61	40370.0
62	34898.0
63	30147.0
64	25396.0
65	21592.0
66	17788.0
67	15356.0
68	12924.0
69	11903.5
70	10883.0
71	8448.5
72	6014.0
73	5294.5
74	4575.0
75	3282.0
76	1989.0
77	1768.5
78	1548.0
79	1346.0
80	1144.0
81	915.5
82	687.0
83	570.0
84	453.0
85	375.5
86	298.0
87	224.0
88	150.0
89	97.0
90	44.0
91	27.5
92	11.0
93	11.0
94	11.0
95	7.5
96	4.0
97	5.5
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01659046543796674
2	0.004416942097121016
3	5.386514752586605E-4
4	1.0773029505173208E-4
5	0.0
6	4.3092118020692834E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	3.2319088515519626E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	928244.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.675577535378245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.92027116191197	51.34400306927625
2	11.865055572512661	14.872984209931781
3	2.998544621979156	5.638065478444381
4	1.1217693323879583	2.8123016307556385
5	0.5731192861314931	1.796029112747751
6	0.3172693449896494	1.1931023658898465
7	0.21573443964476957	0.9464896419304992
8	0.15056492781241876	0.7549395045772703
9	0.10873221711728888	0.6133369054075398
>10	0.5888202546388721	6.953080347200339
>50	0.06911319251797833	3.074900092761066
>100	0.06616828073824778	7.9985568172932435
>500	0.0046646044883789034	1.8864591948500178
>1k	1.7276312919921864E-4	0.1157516289343743
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1069	0.11516368541030161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0773029505173208E-4
2	0.0	0.0	0.0	0.0	1.0773029505173208E-4
3	0.0	0.0	0.0	0.0	1.0773029505173208E-4
4	0.0	0.0	0.0	0.0	1.0773029505173208E-4
5	0.0	0.0	0.0	0.0	1.0773029505173208E-4
6	0.0	0.0	0.0	0.0	1.0773029505173208E-4
7	0.0	0.0	0.0	0.0	1.0773029505173208E-4
8	0.0	0.0	0.0	0.0	1.0773029505173208E-4
9	0.0	0.0	0.0	0.0	1.0773029505173208E-4
10	0.0	0.0	0.0	0.0	1.0773029505173208E-4
11	0.0	0.0	0.0	0.0	1.0773029505173208E-4
12	0.0	0.0	0.0	0.0	3.2319088515519626E-4
13	0.0	0.0	0.0	0.0	3.2319088515519626E-4
14	0.0	0.0	0.0	0.0	3.2319088515519626E-4
15	0.0	0.0	0.0	0.0	5.386514752586605E-4
16	0.0	0.0	0.0	0.0	6.463817703103925E-4
17	0.0	0.0	0.0	0.0	6.463817703103925E-4
18	0.0	0.0	0.0	0.0	6.463817703103925E-4
19	0.0	0.0	0.0	2.1546059010346417E-4	6.463817703103925E-4
20	0.0	0.0	0.0	2.1546059010346417E-4	7.541120653621246E-4
21	0.0	0.0	0.0	2.1546059010346417E-4	8.618423604138567E-4
22	0.0	0.0	0.0	4.3092118020692834E-4	8.618423604138567E-4
23	0.0	0.0	0.0	0.0017236847208277133	8.618423604138567E-4
24	0.0	0.0	0.0	0.0034473694416554267	8.618423604138567E-4
25	0.0	0.0	0.0	0.0040937512119658195	8.618423604138567E-4
26	0.0	0.0	0.0	0.005709705637741801	8.618423604138567E-4
27	0.0	0.0	0.0	0.006679278293207389	8.618423604138567E-4
28	0.0	0.0	0.0	0.012065793045793994	8.618423604138567E-4
29	0.0	0.0	0.0	0.01809868956869099	8.618423604138567E-4
30	0.0	0.0	0.0	0.02984129172932979	8.618423604138567E-4
31	0.0	0.0	0.0	0.06108307729433209	8.618423604138567E-4
32	0.0	0.0	0.0	0.09609542318614502	8.618423604138567E-4
33	0.0	0.0	0.0	0.13369329615919953	8.618423604138567E-4
34	0.0	0.0	0.0	0.17215301149266787	9.695726554655887E-4
35	0.0	0.0	0.0	0.21933888072532653	9.695726554655887E-4
36	0.0	0.0	0.0	0.2890403816237972	9.695726554655887E-4
37	0.0	0.0	0.0	0.3901991286773736	9.695726554655887E-4
38	0.0	0.0	0.0	0.5268011428029699	0.001077302950517321
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGC	75	2.0635416E-6	23.466494	36
TTAACGG	85	6.1113005E-6	20.70573	35
GATATAC	435	0.0	19.729305	1
CCGGTTT	195	0.0	19.179346	22
CGCAATA	235	0.0	18.723267	36
AGTCCGT	60	0.007411152	18.333199	6
GTAGAAC	675	0.0	18.251717	3
GTCCTAC	780	0.0	18.056013	1
AATGCCG	195	1.8189894E-12	18.05115	18
GTAGGAC	845	0.0	17.964365	3
GCATTCG	310	0.0	17.741806	22
GTATTAG	300	0.0	17.604612	1
CTATATA	100	2.4764233E-5	17.604612	1
CTGTAGG	780	0.0	17.491762	1
ATACACT	535	0.0	17.2709	4
GGACGTG	820	0.0	17.170607	6
TAGGACG	770	0.0	17.14273	4
ATACGAA	270	0.0	17.110987	40
TGTAGGA	880	0.0	17.00079	2
TGTAGAA	725	0.0	16.993895	2
>>END_MODULE