FastQCFastQC Report
Thu 2 Feb 2017
SRR4062708_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062708_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149752
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG62464.170895881190235No Hit
TCCATGTACTCTGCGTTGATACCAC52523.5071317912281637No Hit
GTATCAACGCAGAGTACTTTTTTTT37772.522169987713019No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35202.350552914151397No Hit
GAGTACATGGAAGCAGTGGTATCAA27461.8336983813237888No Hit
TATCAACGCAGAGTACTTTTTTTTT27301.8230140498958276No Hit
CATGTACTCTGCGTTGATACCACTG25881.728190608472675No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25241.6854532827608313No Hit
CCCATGTACTCTGCGTTGATACCAC17741.1846252470751641No Hit
GTACATGGGAAGCAGTGGTATCAAC17171.1465623163630536No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17041.1378812970778354No Hit
GCTTCCATGTACTCTGCGTTGATAC16411.0958117420802393No Hit
GCGTTGATACCACTGCTTCCATGTA14620.9762807842299268No Hit
CATGGAAGCAGTGGTATCAACGCAG13900.9282012928041029No Hit
GGTATCAACGCAGAGTACTTTTTTT13560.9054970885196859No Hit
GTATCAACGCAGAGTACATGGAAGC11030.7365510978150542No Hit
ACTCTGCGTTGATACCACTGCTTCC10700.714514664244885No Hit
GTACATGGGGTGGTATCAACGCAAA10200.6811261285325071No Hit
ACGCAGAGTACATGGAAGCAGTGGT9360.6250333885357123No Hit
CAGTGGTATCAACGCAGAGTACATG9160.6116779742507613No Hit
GTATCAACGCAGAGTACATGGGAAG8740.5836316042523639No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8260.5515786099684813No Hit
TATCAACGCAGAGTACATGGAAGCA7480.4994924942571719No Hit
GAGTACATGGGAAGCAGTGGTATCA6200.4140178428334847No Hit
GCGTTGATACCACTGCTTCCCATGT5910.39465249212030556No Hit
GTACATGGGTGGTATCAACGCAAAA5700.3806293071211069No Hit
TATCAACGCAGAGTACATGGGAAGC5660.3779582242641167No Hit
ATACCACTGCTTCCATGTACTCTGC5420.3619317271221753No Hit
GGTATCAACGCAGAGTACATGGAAG5380.3592606442651851No Hit
AAGCAGTGGTATCAACGCAGAGTAC5160.34456968855173886No Hit
GGTATCAACGCAGAGTACATGGGAA4870.3252043378385598No Hit
GTATCAACGCAGAGTACATGGGGTG4850.32386879641006466No Hit
GTACTCTGCGTTGATACCACTGCTT4830.32253325498156954No Hit
GCTTCCCATGTACTCTGCGTTGATA4660.3111811528393611No Hit
GTACATGGGAGTGGTATCAACGCAA4490.2998290506971526No Hit
ACGCAGAGTACATGGGAAGCAGTGG4260.28447032426945884No Hit
AAAAAGTACTCTGCGTTGATACCAC4250.2838025535552113No Hit
CCACTGCTTCCATGTACTCTGCGTT4090.2731182221272504No Hit
ATCAACGCAGAGTACTTTTTTTTTT4080.27245045141300284No Hit
GTGGTATCAACGCAGAGTACATGGA3530.23572306212938723No Hit
CTGCTTCCATGTACTCTGCGTTGAT3340.2230354185586837No Hit
GAAGCAGTGGTATCAACGCAGAGTA3340.2230354185586837No Hit
TATCAACGCAGAGTACATGGGGTGG3330.22236764784443613No Hit
GTTGATACCACTGCTTCCATGTACT3220.215022169987713No Hit
GTATCAACGCAGAGTACATGGGCAG3180.21235108713072282No Hit
ACATGGAAGCAGTGGTATCAACGCA3120.20834446284523747No Hit
TGGAAGCAGTGGTATCAACGCAGAG2930.19565681927453388No Hit
CCATGTACTCTGCGTTGATACCACT2640.17629146856135477No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2510.16761044927613655No Hit
GTATCAACGCAGAGTACATGGGAGT2500.166942678561889No Hit
GATACCACTGCTTCCATGTACTCTG2480.16560713713339387No Hit
GCAGAGTACATGGAAGCAGTGGTAT2380.15892942999091833No Hit
GCAGTGGTATCAACGCAGAGTACAT2350.15692611784817564No Hit
GGTATCAACGCAGAGTACATGGGGT2340.15625834713392808No Hit
CCCCATGTACTCTGCGTTGATACCA2320.154922805705433No Hit
CTTCCATGTACTCTGCGTTGATACC2310.15425503499118542No Hit
ACGCAGAGTACATGGGGTGGTATCA2310.15425503499118542No Hit
GTGGTATCAACGCAGAGTACATGGG2080.13889630856349164No Hit
CTGCTTCCCATGTACTCTGCGTTGA1990.13288637213526364No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1970.1315508307067685No Hit
GAGTACATGGGGTGGTATCAACGCA1880.12554089427854054No Hit
ATCAACGCAGAGTACATGGAAGCAG1770.1181954164218174No Hit
AAAGTACTCTGCGTTGATACCACTG1750.1168598749933223No Hit
TATCAACGCAGAGTACATGGGAGTG1720.11485656285057963No Hit
GTATCAACGCAGAGTACATGGGTGG1710.11418879213633208No Hit
GATACCACTGCTTCCCATGTACTCT1700.1135210214220845No Hit
CAACGCAGAGTACTTTTTTTTTTTT1650.11018216785084672No Hit
TGATACCACTGCTTCCATGTACTCT1620.10817885570810405No Hit
CACTGCTTCCATGTACTCTGCGTTG1500.1001656071371334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCCG250.00599799219.0050216
CTGCCGA250.00599799219.0050217
AGGATCG350.002158896816.2845695
AAAAAGT807.536073E-915.4364131
AAAAGTA902.237357E-914.7717952
AGTTCAT400.005252072614.2489976
GGAGTGG907.3408555E-611.6102938
GGGAGTG1105.931768E-610.3629067
AAGTACT1303.743953E-710.2300484
GGGAAGC4900.010.0809237
GAGTGGT856.5232156E-410.0581159
AAAGTAC1356.338032E-79.8445713
AGTACTC1401.0319854E-69.4993315
TCCATGT11250.09.3726731
TGGGAGT1356.674201E-58.443856
TGGGAAG6100.08.4092446
CTCTGCG18900.08.3433289
TCTGCGT19050.08.22776710
ACATGGA17100.08.21614553
TGCGTTG18950.08.17092812
Produced by FastQC (version 0.11.5)