##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062708_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	149752
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.309217906939473	32.0	32.0	32.0	21.0	32.0
2	30.506350499492495	32.0	32.0	32.0	32.0	32.0
3	30.520994711255945	32.0	32.0	32.0	32.0	32.0
4	30.311862278967894	32.0	32.0	32.0	21.0	32.0
5	30.405463699983972	32.0	32.0	32.0	32.0	32.0
6	33.72401036380148	36.0	36.0	36.0	32.0	36.0
7	33.39968080559859	36.0	36.0	36.0	21.0	36.0
8	33.34454297772317	36.0	36.0	36.0	21.0	36.0
9	33.33489369090229	36.0	36.0	36.0	21.0	36.0
10	33.348923553608635	36.0	36.0	36.0	21.0	36.0
11	33.72819060847267	36.0	36.0	36.0	32.0	36.0
12	33.603270740958386	36.0	36.0	36.0	27.0	36.0
13	33.613140392114964	36.0	36.0	36.0	27.0	36.0
14	33.62195496554303	36.0	36.0	36.0	27.0	36.0
15	33.652018003098455	36.0	36.0	36.0	27.0	36.0
16	33.48631737806507	36.0	36.0	36.0	27.0	36.0
17	33.40134355467707	36.0	36.0	36.0	27.0	36.0
18	33.49753592606443	36.0	36.0	36.0	27.0	36.0
19	33.492968374378975	36.0	36.0	36.0	27.0	36.0
20	33.33831267695924	36.0	36.0	36.0	21.0	36.0
21	33.16909290026177	36.0	36.0	36.0	21.0	36.0
22	33.18817778727496	36.0	36.0	36.0	21.0	36.0
23	33.087431219616434	36.0	36.0	36.0	14.0	36.0
24	33.1191369731289	36.0	36.0	36.0	21.0	36.0
25	32.56488060259629	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	5.0
5	17.0
6	27.0
7	8.0
8	20.0
9	22.0
10	28.0
11	1.0
12	11.0
13	6.0
14	61.0
15	77.0
16	121.0
17	164.0
18	253.0
19	399.0
20	609.0
21	902.0
22	1257.0
23	1656.0
24	1898.0
25	2238.0
26	2599.0
27	3117.0
28	3700.0
29	4824.0
30	6122.0
31	8183.0
32	11376.0
33	15792.0
34	30489.0
35	53769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.37777659048714	15.599663403101498	13.654213471890152	19.368346534521216
2	11.381272287740574	24.13353278217782	37.69583160818987	26.789363321891734
3	21.32091221743631	32.51861497879729	26.57985241577348	19.580620387992923
4	11.84331797235023	17.326521071261606	31.90676551125359	38.923395445134574
5	10.469675816137716	40.924789076747345	32.42707032111103	16.178464786003914
6	29.89344289674984	30.961686207702844	21.136386411464077	18.008484484083244
7	24.369040380079248	33.973926349622126	23.294554736624058	18.36247853367457
8	32.25364445127698	26.55352881806819	23.623931395418786	17.568895335236046
9	28.85998449322247	15.332326283987916	20.06991417800711	35.7377750447825
10	17.028445365511246	28.355115820436538	31.56599926463215	23.050439549420062
11	32.62735659847573	24.23719748629496	18.957079823505815	24.178366091723493
12	22.1782416539071	26.593162656040008	30.761361584905156	20.467234105147732
13	33.728665120103756	21.464242975283966	26.293129383135334	18.513962521476945
14	27.166246598787264	17.525855902232266	28.576872421930887	26.731025077049587
15	27.373304543722547	21.038698852203673	34.54619596098696	17.041800643086816
16	23.706130088909685	25.058493214787088	30.364329166388128	20.871047529915103
17	20.837567524201745	23.7959298283147	33.24998662887094	22.11651601861261
18	24.185999866283346	19.77602460386441	37.4440061509661	18.59396937888614
19	20.021128361482194	31.50666613177144	31.864377315093407	16.60782819165296
20	21.483680318771977	18.204367069143032	41.32268977228662	18.98926283979836
21	21.869713766105253	25.413370954046123	29.238514873330974	23.478400406517654
22	20.407262513792755	26.063463403216637	30.61022503092921	22.91904905206139
23	18.488850255758752	25.601283808632274	30.88763331215941	25.022232623449568
24	17.940916871723548	30.450946827859205	30.73914090082379	20.86899539959345
25	24.52898308484322	22.9899043925921	28.395400147088317	24.085712375476366
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	116.0
1	116.0
2	348.5
3	581.0
4	581.0
5	581.0
6	2193.5
7	3806.0
8	3806.0
9	3806.0
10	3412.0
11	3018.0
12	3018.0
13	3018.0
14	1757.5
15	497.0
16	497.0
17	497.0
18	1050.0
19	1603.0
20	1603.0
21	1603.0
22	2279.5
23	2956.0
24	2956.0
25	2956.0
26	4160.5
27	5365.0
28	5365.0
29	5365.0
30	5952.5
31	6540.0
32	6540.0
33	6540.0
34	6066.0
35	5592.0
36	5592.0
37	5592.0
38	6052.0
39	6512.0
40	6512.0
41	6512.0
42	10936.0
43	15360.0
44	15360.0
45	15360.0
46	30247.5
47	45135.0
48	45135.0
49	45135.0
50	34334.5
51	23534.0
52	23534.0
53	23534.0
54	16878.0
55	10222.0
56	10222.0
57	10222.0
58	8623.5
59	7025.0
60	7025.0
61	7025.0
62	5970.0
63	4915.0
64	4915.0
65	4915.0
66	4051.0
67	3187.0
68	3187.0
69	3187.0
70	2504.5
71	1822.0
72	1822.0
73	1822.0
74	1540.5
75	1259.0
76	1259.0
77	1259.0
78	847.5
79	436.0
80	436.0
81	436.0
82	281.0
83	126.0
84	126.0
85	126.0
86	86.5
87	47.0
88	47.0
89	47.0
90	29.5
91	12.0
92	12.0
93	12.0
94	12.0
95	12.0
96	12.0
97	12.0
98	43.0
99	74.0
100	74.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012019872856456008
2	0.004006624285485336
3	0.004674394999732892
4	0.014690955713446231
5	0.035391847855120465
6	0.04474063785458625
7	0.06610930071050804
8	0.09415567070890539
9	0.09348789999465783
10	0.11151770927934185
11	0.11485656285057963
12	0.12754420642128317
13	0.11552433356482718
14	0.11552433356482718
15	0.1075110849938565
16	0.10817885570810405
17	0.11752764570756985
18	0.12153426999305518
19	0.12687643570703563
20	0.11886318713606496
21	0.12620866499278807
22	0.14490624499171964
23	0.1315508307067685
24	0.1335541428495112
25	0.12153426999305518
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	149752.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.15588439553395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.27037282990229	21.193039158074683
2	13.015842899155677	7.329451359581174
3	4.3567972678114035	3.6800844062182807
4	2.004079309363438	2.257065014156739
5	1.1099516174935964	1.5625834713392808
6	0.6593302343231192	1.1138415513649234
7	0.46722322360307367	0.9208558149473797
8	0.4055592448534295	0.9135103370906567
9	0.246655914998577	0.6250333885357123
>10	1.9637605540271323	10.832576526523853
>50	0.2751162128830282	5.327474758267002
>100	0.1541599468741106	9.611891660879321
>500	0.028460297884451192	5.504433997542604
>1k	0.03794706384593492	21.45013088305999
>5k	0.004743382980741865	7.6780276724183985
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6246	4.170895881190235	No Hit
TCCATGTACTCTGCGTTGATACCAC	5252	3.5071317912281637	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3777	2.522169987713019	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3520	2.350552914151397	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2746	1.8336983813237888	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2730	1.8230140498958276	No Hit
CATGTACTCTGCGTTGATACCACTG	2588	1.728190608472675	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2524	1.6854532827608313	No Hit
CCCATGTACTCTGCGTTGATACCAC	1774	1.1846252470751641	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1717	1.1465623163630536	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1704	1.1378812970778354	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1641	1.0958117420802393	No Hit
GCGTTGATACCACTGCTTCCATGTA	1462	0.9762807842299268	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1390	0.9282012928041029	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1356	0.9054970885196859	No Hit
GTATCAACGCAGAGTACATGGAAGC	1103	0.7365510978150542	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1070	0.714514664244885	No Hit
GTACATGGGGTGGTATCAACGCAAA	1020	0.6811261285325071	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	936	0.6250333885357123	No Hit
CAGTGGTATCAACGCAGAGTACATG	916	0.6116779742507613	No Hit
GTATCAACGCAGAGTACATGGGAAG	874	0.5836316042523639	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	826	0.5515786099684813	No Hit
TATCAACGCAGAGTACATGGAAGCA	748	0.4994924942571719	No Hit
GAGTACATGGGAAGCAGTGGTATCA	620	0.4140178428334847	No Hit
GCGTTGATACCACTGCTTCCCATGT	591	0.39465249212030556	No Hit
GTACATGGGTGGTATCAACGCAAAA	570	0.3806293071211069	No Hit
TATCAACGCAGAGTACATGGGAAGC	566	0.3779582242641167	No Hit
ATACCACTGCTTCCATGTACTCTGC	542	0.3619317271221753	No Hit
GGTATCAACGCAGAGTACATGGAAG	538	0.3592606442651851	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	516	0.34456968855173886	No Hit
GGTATCAACGCAGAGTACATGGGAA	487	0.3252043378385598	No Hit
GTATCAACGCAGAGTACATGGGGTG	485	0.32386879641006466	No Hit
GTACTCTGCGTTGATACCACTGCTT	483	0.32253325498156954	No Hit
GCTTCCCATGTACTCTGCGTTGATA	466	0.3111811528393611	No Hit
GTACATGGGAGTGGTATCAACGCAA	449	0.2998290506971526	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	426	0.28447032426945884	No Hit
AAAAAGTACTCTGCGTTGATACCAC	425	0.2838025535552113	No Hit
CCACTGCTTCCATGTACTCTGCGTT	409	0.2731182221272504	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	408	0.27245045141300284	No Hit
GTGGTATCAACGCAGAGTACATGGA	353	0.23572306212938723	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	334	0.2230354185586837	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	334	0.2230354185586837	No Hit
TATCAACGCAGAGTACATGGGGTGG	333	0.22236764784443613	No Hit
GTTGATACCACTGCTTCCATGTACT	322	0.215022169987713	No Hit
GTATCAACGCAGAGTACATGGGCAG	318	0.21235108713072282	No Hit
ACATGGAAGCAGTGGTATCAACGCA	312	0.20834446284523747	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	293	0.19565681927453388	No Hit
CCATGTACTCTGCGTTGATACCACT	264	0.17629146856135477	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	251	0.16761044927613655	No Hit
GTATCAACGCAGAGTACATGGGAGT	250	0.166942678561889	No Hit
GATACCACTGCTTCCATGTACTCTG	248	0.16560713713339387	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	238	0.15892942999091833	No Hit
GCAGTGGTATCAACGCAGAGTACAT	235	0.15692611784817564	No Hit
GGTATCAACGCAGAGTACATGGGGT	234	0.15625834713392808	No Hit
CCCCATGTACTCTGCGTTGATACCA	232	0.154922805705433	No Hit
CTTCCATGTACTCTGCGTTGATACC	231	0.15425503499118542	No Hit
ACGCAGAGTACATGGGGTGGTATCA	231	0.15425503499118542	No Hit
GTGGTATCAACGCAGAGTACATGGG	208	0.13889630856349164	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	199	0.13288637213526364	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	197	0.1315508307067685	No Hit
GAGTACATGGGGTGGTATCAACGCA	188	0.12554089427854054	No Hit
ATCAACGCAGAGTACATGGAAGCAG	177	0.1181954164218174	No Hit
AAAGTACTCTGCGTTGATACCACTG	175	0.1168598749933223	No Hit
TATCAACGCAGAGTACATGGGAGTG	172	0.11485656285057963	No Hit
GTATCAACGCAGAGTACATGGGTGG	171	0.11418879213633208	No Hit
GATACCACTGCTTCCCATGTACTCT	170	0.1135210214220845	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	165	0.11018216785084672	No Hit
TGATACCACTGCTTCCATGTACTCT	162	0.10817885570810405	No Hit
CACTGCTTCCATGTACTCTGCGTTG	150	0.1001656071371334	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	6.67770714247556E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTGCCG	25	0.005997992	19.00502	16
CTGCCGA	25	0.005997992	19.00502	17
AGGATCG	35	0.0021588968	16.284569	5
AAAAAGT	80	7.536073E-9	15.436413	1
AAAAGTA	90	2.237357E-9	14.771795	2
AGTTCAT	40	0.0052520726	14.248997	6
GGAGTGG	90	7.3408555E-6	11.610293	8
GGGAGTG	110	5.931768E-6	10.362906	7
AAGTACT	130	3.743953E-7	10.230048	4
GGGAAGC	490	0.0	10.080923	7
GAGTGGT	85	6.5232156E-4	10.058115	9
AAAGTAC	135	6.338032E-7	9.844571	3
AGTACTC	140	1.0319854E-6	9.499331	5
TCCATGT	1125	0.0	9.372673	1
TGGGAGT	135	6.674201E-5	8.44385	6
TGGGAAG	610	0.0	8.409244	6
CTCTGCG	1890	0.0	8.343328	9
TCTGCGT	1905	0.0	8.227767	10
ACATGGA	1710	0.0	8.2161455	3
TGCGTTG	1895	0.0	8.170928	12
>>END_MODULE