FastQCFastQC Report
Thu 2 Feb 2017
SRR4062707_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062707_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302725
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG33690.2586117561265808No Hit
TCCATGTACTCTGCGTTGATACCAC27940.2144735074555259No Hit
GTATCAACGCAGAGTACTTTTTTTT23870.18323130361357923No Hit
TATCAACGCAGAGTACTTTTTTTTT15170.11644821431998312No Hit
GAGTACATGGAAGCAGTGGTATCAA14330.110000191905429No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13740.10547122378092076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT1257.2759576E-1213.6786994
GCGTTAT707.305096E-613.5633621
AAGACGG2200.013.3856065
TAGATCG500.001499958313.2992475
CTAGGAT655.4638964E-513.1505743
GTATTAG1650.012.6591391
CCAACGA1301.9645086E-1012.42428319
CAAGACG2150.012.3709174
AACCGCG1001.4391662E-712.3516747
CCGCTCA701.0892582E-412.2159419
TACCGTG550.003064870412.0925487
GCATCGC2250.011.82291514
CTTATAC658.0386415E-411.6893993
CGCATCG2200.011.66022313
CGCCAGT2050.011.58649218
CGCAAGA2050.011.580262
ACGCTAT1501.7644197E-1011.40154510
ATACGCT1501.7644197E-1011.4015458
ATATACG1501.7644197E-1011.4011076
ACGCTTA752.0714513E-411.40110717
Produced by FastQC (version 0.11.5)