##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062707_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1302725 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.151573816423266 32.0 32.0 32.0 32.0 32.0 2 30.922583814696118 32.0 32.0 32.0 32.0 32.0 3 30.921031683586328 32.0 32.0 32.0 32.0 32.0 4 30.89929954518413 32.0 32.0 32.0 32.0 32.0 5 30.857777351321268 32.0 32.0 32.0 32.0 32.0 6 34.3827484695542 36.0 36.0 36.0 32.0 36.0 7 34.3134874973613 36.0 36.0 36.0 32.0 36.0 8 34.307957550519106 36.0 36.0 36.0 32.0 36.0 9 34.39297319081157 36.0 36.0 36.0 32.0 36.0 10 34.19169586827611 36.0 36.0 36.0 32.0 36.0 11 34.403731409161566 36.0 36.0 36.0 32.0 36.0 12 34.23612504557754 36.0 36.0 36.0 32.0 36.0 13 34.318528469170396 36.0 36.0 36.0 32.0 36.0 14 34.24933581531022 36.0 36.0 36.0 32.0 36.0 15 34.20760099023202 36.0 36.0 36.0 32.0 36.0 16 34.20946017002821 36.0 36.0 36.0 32.0 36.0 17 34.14861233184287 36.0 36.0 36.0 32.0 36.0 18 34.16284403845785 36.0 36.0 36.0 32.0 36.0 19 34.140057187817845 36.0 36.0 36.0 32.0 36.0 20 34.10918574526473 36.0 36.0 36.0 32.0 36.0 21 34.072907942965706 36.0 36.0 36.0 32.0 36.0 22 34.03396649331209 36.0 36.0 36.0 32.0 36.0 23 34.001028613099464 36.0 36.0 36.0 32.0 36.0 24 33.97495250340632 36.0 36.0 36.0 32.0 36.0 25 33.41392772841544 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 45.0 5 116.0 6 200.0 7 57.0 8 180.0 9 246.0 10 164.0 11 45.0 12 98.0 13 67.0 14 281.0 15 396.0 16 684.0 17 996.0 18 1295.0 19 1634.0 20 2183.0 21 2802.0 22 3639.0 23 5101.0 24 6780.0 25 9287.0 26 12648.0 27 16967.0 28 23181.0 29 31830.0 30 42278.0 31 60634.0 32 91032.0 33 136239.0 34 321314.0 35 530301.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.823644429977726 17.04950149968486 10.832507425493644 26.294346644843763 2 16.93158125838653 20.246736737382204 36.78006331543183 26.041618688799435 3 19.576391799586386 23.802878097685824 28.30932871511193 28.31140138761586 4 12.958053785308651 15.267832534515016 35.52572686325044 36.24838681692589 5 14.615543503550205 36.40943578339869 33.761159900147355 15.213860812903752 6 34.22593975175456 34.98681824848965 16.868187860501514 13.91905413925428 7 29.737655909386284 30.36674711455298 21.036149316602632 18.859447659458105 8 27.99710511475823 32.832516137755974 19.940365978644845 19.230012768840954 9 27.52567996490206 14.08020431793752 18.344511452545945 40.04960426461447 10 15.682983511011104 26.88851795836561 32.12693816321763 25.301560367405656 11 37.60452875314038 20.97922143995106 22.030099977174864 19.386149829733696 12 24.137737553083145 23.696393228416056 29.32979765953767 22.836071558963127 13 30.039432740318002 19.512481910970077 25.007704408920052 25.44038093979187 14 23.352260238282252 19.45853885728427 25.137071473916073 32.052129430517404 15 25.207374334304177 27.054877158468265 22.58478255857739 25.152965948650163 16 25.736843480412574 25.523133127486947 24.147579255725454 24.592444136375025 17 24.05071852265164 25.531802063110714 25.638247016851352 24.7792323973863 18 24.73287289038373 24.978440755503563 26.56214212038436 23.72654423372834 19 25.462301457674407 25.208198025433404 25.255852026640124 24.07364849025207 20 25.44692297346629 24.42354026168655 25.5748842392453 24.55465252560186 21 26.30407447374327 24.59825559767454 24.121726939855932 24.975942988726256 22 25.39415189213664 24.934445750447214 24.839045194956146 24.83235716246 23 24.46357935581865 24.582647289878533 25.238942978066596 25.714830376236225 24 24.435328534029402 25.296728264395224 25.209335430146457 25.05860777142892 25 24.958110692303435 24.685100011682888 25.214980538765673 25.141808757248 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 120.0 1 120.0 2 369.5 3 619.0 4 619.0 5 619.0 6 1610.0 7 2601.0 8 2601.0 9 2601.0 10 2867.5 11 3134.0 12 3134.0 13 3134.0 14 3643.5 15 4153.0 16 4153.0 17 4153.0 18 6378.0 19 8603.0 20 8603.0 21 8603.0 22 13831.0 23 19059.0 24 19059.0 25 19059.0 26 28500.5 27 37942.0 28 37942.0 29 37942.0 30 48239.5 31 58537.0 32 58537.0 33 58537.0 34 70640.5 35 82744.0 36 82744.0 37 82744.0 38 94112.0 39 105480.0 40 105480.0 41 105480.0 42 120588.0 43 135696.0 44 135696.0 45 135696.0 46 155396.5 47 175097.0 48 175097.0 49 175097.0 50 172629.5 51 170162.0 52 170162.0 53 170162.0 54 158289.0 55 146416.0 56 146416.0 57 146416.0 58 133920.5 59 121425.0 60 121425.0 61 121425.0 62 108268.0 63 95111.0 64 95111.0 65 95111.0 66 79085.0 67 63059.0 68 63059.0 69 63059.0 70 48526.5 71 33994.0 72 33994.0 73 33994.0 74 26630.0 75 19266.0 76 19266.0 77 19266.0 78 15376.0 79 11486.0 80 11486.0 81 11486.0 82 8091.0 83 4696.0 84 4696.0 85 4696.0 86 3396.0 87 2096.0 88 2096.0 89 2096.0 90 1340.0 91 584.0 92 584.0 93 584.0 94 342.0 95 100.0 96 100.0 97 100.0 98 322.5 99 545.0 100 545.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009057936249016485 2 0.0031472490356752193 3 0.004528968124508243 4 0.013510142201922894 5 0.03116544167034485 6 0.0419121456946017 7 0.06501755934675392 8 0.08589687002245294 9 0.09395689804064557 10 0.11460592220153908 11 0.1172925982076033 12 0.13049569172311884 13 0.1168320251779923 14 0.12120746895929685 15 0.11168895968066937 16 0.10984666756222534 17 0.1215145176457042 18 0.12734844268744364 19 0.12896044829108216 20 0.11898136598284366 21 0.12658082097142528 22 0.14538755301387477 23 0.13717400065247845 24 0.13072597823792434 25 0.12873016177627666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1302725.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.19806718172732 #Duplication Level Percentage of deduplicated Percentage of total 1 80.22969436627085 45.889834483314104 2 12.220786089661031 13.980106875399006 3 3.3766441432072267 5.794125556558592 4 1.3783167265666219 3.1534821089542455 5 0.7075778265540258 2.0236042029768884 6 0.4660514913935424 1.5994346708923233 7 0.2848120570192039 1.1403489420085267 8 0.19538557448245358 0.8940541772470214 9 0.1540533341896445 0.7930397662693541 >10 0.8156824275440832 8.496809533645491 >50 0.08380504714025278 3.3762391355391523 >100 0.07993621786575043 9.141541096790789 >500 0.005373850448393416 2.0587132639281194 >1k 0.0018808476569376952 1.6586661864763712 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGAAGCAGTGGTATCAACG 3369 0.2586117561265808 No Hit TCCATGTACTCTGCGTTGATACCAC 2794 0.2144735074555259 No Hit GTATCAACGCAGAGTACTTTTTTTT 2387 0.18323130361357923 No Hit TATCAACGCAGAGTACTTTTTTTTT 1517 0.11644821431998312 No Hit GAGTACATGGAAGCAGTGGTATCAA 1433 0.110000191905429 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1374 0.10547122378092076 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.676217160183461E-5 7 0.0 0.0 0.0 0.0 7.676217160183461E-5 8 0.0 0.0 0.0 0.0 7.676217160183461E-5 9 0.0 0.0 0.0 0.0 7.676217160183461E-5 10 0.0 0.0 0.0 0.0 7.676217160183461E-5 11 0.0 0.0 0.0 0.0 7.676217160183461E-5 12 0.0 0.0 0.0 0.0 7.676217160183461E-5 13 0.0 0.0 0.0 7.676217160183461E-5 7.676217160183461E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATACT 125 7.2759576E-12 13.678699 4 GCGTTAT 70 7.305096E-6 13.563362 1 AAGACGG 220 0.0 13.385606 5 TAGATCG 50 0.0014999583 13.299247 5 CTAGGAT 65 5.4638964E-5 13.150574 3 GTATTAG 165 0.0 12.659139 1 CCAACGA 130 1.9645086E-10 12.424283 19 CAAGACG 215 0.0 12.370917 4 AACCGCG 100 1.4391662E-7 12.351674 7 CCGCTCA 70 1.0892582E-4 12.215941 9 TACCGTG 55 0.0030648704 12.092548 7 GCATCGC 225 0.0 11.822915 14 CTTATAC 65 8.0386415E-4 11.689399 3 CGCATCG 220 0.0 11.660223 13 CGCCAGT 205 0.0 11.586492 18 CGCAAGA 205 0.0 11.58026 2 ACGCTAT 150 1.7644197E-10 11.401545 10 ATACGCT 150 1.7644197E-10 11.401545 8 ATATACG 150 1.7644197E-10 11.401107 6 ACGCTTA 75 2.0714513E-4 11.401107 17 >>END_MODULE