FastQCFastQC Report
Thu 2 Feb 2017
SRR4062707_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062707_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302725
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17690.13579228156364542No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC14540.11161219750906753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA500.00258063621.9998654
TAGGACG3250.019.6306484
ATACGAA3950.018.37963540
CGCATAG600.00741102418.3339232
AGACCGT1203.1626405E-718.3332216
TACGAAT4000.018.14988941
CCTTATA1102.7959013E-618.0005822
CGTCGTA3200.017.87489110
ATACCGT3600.017.7221136
GTCCTAT3850.017.7182581
AGGACGT3500.017.5998945
GTCGTAG3400.017.4704811
CCGTCGT3300.017.3332279
GATATAC4200.017.289591
TACCGTC3700.017.2431377
GGACGTG3500.016.9713256
CTAGGAC2350.016.8509623
ACGTTTT3150.016.76180329
ACCGTCC1053.7749047E-516.76188
CGCAATA4200.016.761836
Produced by FastQC (version 0.11.5)