##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062707_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1302725
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24284557370128	32.0	32.0	32.0	32.0	32.0
2	31.282004260300525	32.0	32.0	32.0	32.0	32.0
3	31.404965744880922	32.0	32.0	32.0	32.0	32.0
4	31.541382870521407	32.0	32.0	32.0	32.0	32.0
5	31.439164827572974	32.0	32.0	32.0	32.0	32.0
6	35.0310433898175	36.0	36.0	36.0	36.0	36.0
7	35.04652939031645	36.0	36.0	36.0	36.0	36.0
8	34.97677637260358	36.0	36.0	36.0	36.0	36.0
9	35.12975033103687	36.0	36.0	36.0	36.0	36.0
10	34.98332111535435	36.0	36.0	36.0	36.0	36.0
11	35.13598418699265	36.0	36.0	36.0	36.0	36.0
12	35.03275595386594	36.0	36.0	36.0	36.0	36.0
13	35.0838480876624	36.0	36.0	36.0	36.0	36.0
14	35.04434934464296	36.0	36.0	36.0	36.0	36.0
15	34.99650885643555	36.0	36.0	36.0	36.0	36.0
16	35.01370435049608	36.0	36.0	36.0	36.0	36.0
17	34.99186935078394	36.0	36.0	36.0	36.0	36.0
18	35.00307893070295	36.0	36.0	36.0	36.0	36.0
19	34.99105183365637	36.0	36.0	36.0	36.0	36.0
20	34.97412961292675	36.0	36.0	36.0	36.0	36.0
21	34.96358479341381	36.0	36.0	36.0	32.0	36.0
22	34.94494156479687	36.0	36.0	36.0	32.0	36.0
23	34.901826555873264	36.0	36.0	36.0	32.0	36.0
24	34.88323629315473	36.0	36.0	36.0	32.0	36.0
25	34.85390162927709	36.0	36.0	36.0	32.0	36.0
26	34.81132702604157	36.0	36.0	36.0	32.0	36.0
27	34.79410466522098	36.0	36.0	36.0	32.0	36.0
28	34.76825730679921	36.0	36.0	36.0	32.0	36.0
29	34.75070947437103	36.0	36.0	36.0	32.0	36.0
30	34.7283432804314	36.0	36.0	36.0	32.0	36.0
31	34.72285708802702	36.0	36.0	36.0	32.0	36.0
32	34.68438081713332	36.0	36.0	36.0	32.0	36.0
33	34.66325356464334	36.0	36.0	36.0	32.0	36.0
34	34.648712506476805	36.0	36.0	36.0	32.0	36.0
35	34.617282235314434	36.0	36.0	36.0	32.0	36.0
36	34.58226717073826	36.0	36.0	36.0	32.0	36.0
37	34.574909516590225	36.0	36.0	36.0	32.0	36.0
38	34.524824502485174	36.0	36.0	36.0	32.0	36.0
39	34.50131992554069	36.0	36.0	36.0	32.0	36.0
40	34.48612178318525	36.0	36.0	36.0	32.0	36.0
41	34.460228367460516	36.0	36.0	36.0	32.0	36.0
42	34.411583411694714	36.0	36.0	36.0	32.0	36.0
43	34.411975666391605	36.0	36.0	36.0	32.0	36.0
44	34.38760905026003	36.0	36.0	36.0	32.0	36.0
45	34.32490894087394	36.0	36.0	36.0	32.0	36.0
46	34.345927191080236	36.0	36.0	36.0	32.0	36.0
47	34.31619796964056	36.0	36.0	36.0	32.0	36.0
48	34.268475695177415	36.0	36.0	36.0	32.0	36.0
49	34.267063271219946	36.0	36.0	36.0	32.0	36.0
50	33.68662380778752	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	6.0
21	27.0
22	104.0
23	300.0
24	807.0
25	1914.0
26	4233.0
27	8096.0
28	13693.0
29	21668.0
30	32185.0
31	47212.0
32	71497.0
33	117369.0
34	251305.0
35	732308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.22283657223402	17.63700615177448	11.380514083517651	26.75964319247385
2	16.37331863522541	20.75649708059343	36.30634807801256	26.563836206168602
3	18.93421129394281	24.12390728293811	28.662732322317375	28.27914910080171
4	12.864054753005819	15.700574872785698	35.69569279117664	35.73967758303185
5	14.47788289930722	36.53626053081042	33.5395421136464	15.446314456235966
6	34.47952137214022	35.26218907458525	16.482846711966616	13.775442841307905
7	29.768293385019863	30.367498896543783	21.00581473449884	18.858392983937517
8	28.036154982824463	32.999443474255884	19.71797578153486	19.24642576138479
9	27.550173674413248	13.901475752749045	18.1254677694832	40.42288280335451
10	15.580034159166361	26.90675315204667	32.158053311328175	25.355159377458786
11	37.89349248690245	20.843846552418967	21.908537872536414	19.354123088142163
12	24.125736437083805	23.565833157420023	29.285152276957916	23.02327812853826
13	30.205761000978722	19.34637010881038	25.00957608090733	25.438292809303576
14	23.499740927670842	19.289259053138615	24.987698862000805	32.22330115718974
15	25.43061659214339	26.98366884799171	22.268322170834214	25.317392389030687
16	25.886967702316298	25.497246157093784	23.97321000211096	24.642576138478958
17	24.258918804812986	25.472528737837997	25.501084265673875	24.767468191675142
18	25.017022011552708	24.87616342666334	26.32980867028728	23.777005891496668
19	25.70243144178549	25.106756990155247	25.09570323744459	24.09510833061467
20	25.54660423343376	24.45481586674087	25.37028152526435	24.628298374561016
21	26.445873073749254	24.49327371471339	24.028325241321077	25.03252797021628
22	25.5354737185515	24.923295400026866	24.807384520908098	24.73384636051354
23	24.563818150415475	24.402847876566426	25.107294325356467	25.926039647661632
24	24.657084188911703	25.219597382409948	25.011571897368977	25.11174653130937
25	25.222744631445625	24.373755013529333	25.142835210808112	25.260665144216933
26	24.33437601949759	25.063770174058224	25.64132875319043	24.960525053253757
27	25.3556199504884	24.948243105797463	25.080581089639026	24.61555585407511
28	24.548081905237098	24.78957569709647	25.44596902646376	25.21637337120267
29	24.483601681091557	24.935347060968354	25.391698171141265	25.18935308679883
30	24.803239363641598	25.072060488591223	25.44612255080696	24.67857759696022
31	24.88161354084707	24.829875837187434	24.841850735957323	25.446659886008177
32	24.77368592757489	25.144293692068548	24.95200445220595	25.130015928150605
33	24.51676293922355	24.598361127636302	25.390853787253644	25.494022145886508
34	24.796484292540637	24.950315684430713	25.762229173463318	24.490970849565336
35	25.561342570381317	24.811913489032605	25.207315434953653	24.419428505632425
36	24.460342743096202	25.32376364927364	25.236638584505556	24.979255023124605
37	25.322074881498395	24.883762881651922	25.021167168819208	24.772995068030472
38	24.698075188547083	24.836861194803202	25.079352894893397	25.385710721756315
39	25.030762440269434	24.609031069488957	25.073518969851655	25.28668752038995
40	25.552668444990307	24.67489301272333	25.082653668272275	24.689784874014087
41	24.267976741062007	24.844230363276978	25.90922873208083	24.978564163580188
42	25.57400832869562	25.160797558962944	25.279778924945788	23.98541518739565
43	24.658005334970927	24.240802932314956	25.578844345506536	25.522347387207585
44	24.396399854151873	24.772918305858873	25.628816519219328	25.201865320769922
45	24.46272237041586	25.04258381469612	25.46216584467175	25.03252797021628
46	24.303613044666406	24.42136630629358	25.628759430240024	25.646261218799992
47	24.584774223262777	24.397628048897506	26.044944251472874	24.972653476366848
48	24.68026636473546	25.39023968988083	25.011188086510966	24.918305858872746
49	24.1714866913585	24.840776065554895	25.916597900554606	25.071139342532
50	24.0545779040089	25.55896294306166	24.96605192960909	25.420407223320346
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	12.0
2	21.0
3	59.5
4	98.0
5	130.5
6	163.0
7	152.5
8	142.0
9	221.5
10	301.0
11	416.0
12	531.0
13	957.0
14	1383.0
15	1894.0
16	2405.0
17	2892.5
18	3380.0
19	3718.0
20	4056.0
21	4721.5
22	5387.0
23	6066.0
24	6745.0
25	7771.0
26	8797.0
27	11367.5
28	13938.0
29	16224.5
30	18511.0
31	20674.5
32	22838.0
33	26260.0
34	29682.0
35	34450.5
36	39219.0
37	44882.0
38	50545.0
39	53884.0
40	57223.0
41	61527.0
42	65831.0
43	68337.5
44	70844.0
45	78108.0
46	85372.0
47	90907.5
48	96443.0
49	97454.0
50	98465.0
51	94456.5
52	90448.0
53	88159.0
54	85870.0
55	86172.0
56	86474.0
57	84964.5
58	83455.0
59	76827.5
60	70200.0
61	61942.5
62	53685.0
63	47561.5
64	41438.0
65	35769.5
66	30101.0
67	27326.0
68	24551.0
69	22228.0
70	19905.0
71	15853.5
72	11802.0
73	10259.0
74	8716.0
75	6758.0
76	4800.0
77	4163.5
78	3527.0
79	2979.0
80	2431.0
81	1869.0
82	1307.0
83	1084.5
84	862.0
85	680.0
86	498.0
87	365.0
88	232.0
89	147.5
90	63.0
91	45.0
92	27.0
93	16.5
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013356617858719224
2	0.004836016810915581
3	6.908595444165116E-4
4	1.5352434320366922E-4
5	0.0
6	7.676217160183461E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	7.676217160183461E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1302725.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.079731535074224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.95118494604272	49.444766439504306
2	12.188695562938605	14.889645054940809
3	3.1301656413210424	5.735690310966081
4	1.2552563697571244	3.066828882898281
5	0.6456238258291946	1.9717264977147364
6	0.40378774878931845	1.4797948375922136
7	0.24964190521792917	1.067364238543089
8	0.18548507007247803	0.9063502627033111
9	0.14157974658542688	0.7782887621017545
>10	0.7085176694424206	7.90026098928384
>50	0.0723731379582079	3.0690250230823364
>100	0.0632684443126154	7.596693538027928
>500	0.003662229150180158	1.5243949712356475
>1k	7.577025827958947E-4	0.5691701914055478
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1769	0.13579228156364542	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1454	0.11161219750906753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.676217160183461E-5
2	0.0	0.0	0.0	0.0	7.676217160183461E-5
3	0.0	0.0	0.0	0.0	7.676217160183461E-5
4	0.0	0.0	0.0	0.0	7.676217160183461E-5
5	0.0	0.0	0.0	0.0	7.676217160183461E-5
6	0.0	0.0	0.0	0.0	7.676217160183461E-5
7	0.0	0.0	0.0	0.0	7.676217160183461E-5
8	0.0	0.0	0.0	0.0	7.676217160183461E-5
9	0.0	0.0	0.0	0.0	7.676217160183461E-5
10	0.0	0.0	0.0	0.0	7.676217160183461E-5
11	0.0	0.0	0.0	0.0	7.676217160183461E-5
12	0.0	0.0	0.0	0.0	7.676217160183461E-5
13	0.0	0.0	0.0	0.0	7.676217160183461E-5
14	0.0	0.0	0.0	0.0	7.676217160183461E-5
15	0.0	0.0	0.0	0.0	7.676217160183461E-5
16	0.0	0.0	0.0	0.0	7.676217160183461E-5
17	0.0	0.0	0.0	0.0	7.676217160183461E-5
18	0.0	0.0	0.0	0.0	1.5352434320366922E-4
19	0.0	0.0	0.0	7.676217160183461E-5	1.5352434320366922E-4
20	0.0	0.0	0.0	4.605730296110077E-4	1.5352434320366922E-4
21	0.0	0.0	0.0	6.140973728146769E-4	1.5352434320366922E-4
22	0.0	0.0	0.0	9.9790823082385E-4	1.5352434320366922E-4
23	0.0	0.0	0.0	0.0011514325740275193	1.5352434320366922E-4
24	0.0	0.0	0.0	0.0016887677752403616	1.5352434320366922E-4
25	0.0	0.0	0.0	0.0023028651480550386	1.5352434320366922E-4
26	0.0	0.0	0.0	0.003377535550480723	1.5352434320366922E-4
27	0.0	0.0	0.0	0.0049895411541192505	1.5352434320366922E-4
28	0.0	0.0	0.0	0.010669941852655011	1.5352434320366922E-4
29	0.0	0.0	0.0	0.024640657084188913	1.5352434320366922E-4
30	0.0	0.0	0.0	0.0476693085647393	1.5352434320366922E-4
31	0.0	0.0	0.0	0.0980252931355428	1.5352434320366922E-4
32	0.0	0.0	0.0	0.16288932813909304	1.5352434320366922E-4
33	0.0	0.0	0.0	0.22591107102419927	1.5352434320366922E-4
34	0.0	0.0	0.0	0.3017520965668119	1.5352434320366922E-4
35	0.0	0.0	0.0	0.3828897119499511	1.5352434320366922E-4
36	0.0	0.0	0.0	0.5057091865128864	1.5352434320366922E-4
37	0.0	0.0	0.0	0.6841812354871519	1.5352434320366922E-4
38	0.0	0.0	0.0	0.9062541979312595	1.5352434320366922E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	50	0.002580636	21.999865	4
TAGGACG	325	0.0	19.630648	4
ATACGAA	395	0.0	18.379635	40
CGCATAG	60	0.007411024	18.333923	2
AGACCGT	120	3.1626405E-7	18.333221	6
TACGAAT	400	0.0	18.149889	41
CCTTATA	110	2.7959013E-6	18.000582	2
CGTCGTA	320	0.0	17.874891	10
ATACCGT	360	0.0	17.722113	6
GTCCTAT	385	0.0	17.718258	1
AGGACGT	350	0.0	17.599894	5
GTCGTAG	340	0.0	17.47048	11
CCGTCGT	330	0.0	17.333227	9
GATATAC	420	0.0	17.28959	1
TACCGTC	370	0.0	17.243137	7
GGACGTG	350	0.0	16.971325	6
CTAGGAC	235	0.0	16.850962	3
ACGTTTT	315	0.0	16.761803	29
ACCGTCC	105	3.7749047E-5	16.7618	8
CGCAATA	420	0.0	16.7618	36
>>END_MODULE