FastQCFastQC Report
Thu 2 Feb 2017
SRR4062706_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062706_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688215
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24230.14352437337661375No Hit
GCGCAAGACGGACCAGAGCGAAAGC19880.11775751311296251No Hit
GTCTTGCGCCGGTCCAAGAATTTCA17410.10312667521613064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAT3100.012.8727169
TACCGTG951.0393469E-612.0005637
GTATAGG951.0479453E-611.9923791
CGCATCG2950.011.91687313
CCGTCGT2950.011.9168739
ACACCGT1052.720626E-711.7617586
ATACCGT3150.011.7617586
TACCGTC2750.011.7460047
CGGTCCA3550.011.50894610
ACCGTCG3000.011.4008728
TGCACCG752.076541E-411.3985055
TAAGGGT1001.936205E-611.3968144
CGCAAGA3750.011.3930982
GACGGTA3350.011.34415158
GCGTTAT1604.5474735E-1111.2740861
AAGACGG3900.011.2036595
CAAGACG3900.011.2019984
CGTCGTA2900.011.13977410
AGAACCG1451.2205419E-911.1364715
CCTATTC3250.011.1016253
Produced by FastQC (version 0.11.5)