##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062706_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1688215
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20766549284303	32.0	32.0	32.0	32.0	32.0
2	30.85201351723566	32.0	32.0	32.0	32.0	32.0
3	30.836619150996764	32.0	32.0	32.0	32.0	32.0
4	30.91572992776394	32.0	32.0	32.0	32.0	32.0
5	30.71709764455357	32.0	32.0	32.0	32.0	32.0
6	34.367435427359666	36.0	36.0	36.0	32.0	36.0
7	34.26503259359738	36.0	36.0	36.0	32.0	36.0
8	34.26031873902317	36.0	36.0	36.0	32.0	36.0
9	34.427932461209025	36.0	36.0	36.0	32.0	36.0
10	34.101198603258474	36.0	36.0	36.0	32.0	36.0
11	34.38699691686189	36.0	36.0	36.0	32.0	36.0
12	34.16194560526947	36.0	36.0	36.0	32.0	36.0
13	34.29537233113081	36.0	36.0	36.0	32.0	36.0
14	34.194510177909805	36.0	36.0	36.0	32.0	36.0
15	34.13979795227504	36.0	36.0	36.0	32.0	36.0
16	34.137568970776826	36.0	36.0	36.0	32.0	36.0
17	34.072450487645234	36.0	36.0	36.0	32.0	36.0
18	34.079825140755176	36.0	36.0	36.0	32.0	36.0
19	34.07723305384681	36.0	36.0	36.0	32.0	36.0
20	34.07266076891865	36.0	36.0	36.0	32.0	36.0
21	34.05110071880655	36.0	36.0	36.0	32.0	36.0
22	34.00825546509183	36.0	36.0	36.0	32.0	36.0
23	33.98398959848124	36.0	36.0	36.0	32.0	36.0
24	33.935536646694885	36.0	36.0	36.0	32.0	36.0
25	33.50304967080615	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	88.0
5	153.0
6	302.0
7	75.0
8	248.0
9	320.0
10	208.0
11	58.0
12	127.0
13	100.0
14	238.0
15	323.0
16	518.0
17	715.0
18	988.0
19	1360.0
20	2023.0
21	2958.0
22	4551.0
23	6781.0
24	9607.0
25	13972.0
26	19510.0
27	25422.0
28	34735.0
29	46130.0
30	59473.0
31	81224.0
32	116216.0
33	168179.0
34	378038.0
35	713568.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.97040088101291	17.78452978779745	10.923209346603818	26.321859984585828
2	17.200910583129804	20.05456864293377	36.509632739764086	26.23488803417234
3	19.11354485500709	23.416445661053114	28.66554865569152	28.80446082824828
4	12.901738980522445	15.340683897501279	35.83948036159989	35.918096760376386
5	14.813495807003655	36.211939491910286	33.658217466946674	15.31634723413938
6	34.16331237791812	35.42040023894862	16.67923043371072	13.737056949422541
7	30.021748447111086	30.49957410032027	20.935355126830377	18.54332232573827
8	27.871679807812594	32.86282517147521	19.781441369975582	19.48405365073662
9	27.19856795654538	14.270865635641233	18.925066156114795	39.605500251698594
10	16.015756586594804	26.532839269532047	31.763980945964537	25.68742319790861
11	37.28125502252761	21.254417600300805	22.35058147155092	19.113745905620664
12	24.421951089309758	23.971695147485363	28.66566227542069	22.94069148778419
13	29.202777743181525	19.510745695583402	25.296405884051936	25.990070677183137
14	23.07225589341412	20.08852501991309	25.02050618091582	31.818712905756975
15	24.939229815373682	27.554705026653487	22.162748455022946	25.34331670294988
16	25.562435249599268	25.969840175107024	23.87357282259265	24.59415175270106
17	23.997824542341355	26.031474151316488	25.31638437513715	24.654316931205006
18	24.766024483278738	24.886489474586394	26.336636482208725	24.010849559926143
19	25.331564986737398	25.243483769330417	25.40570742563217	24.019243818300016
20	25.350262411385376	24.74329707791919	25.106740850000563	24.799699660694873
21	26.369527849646428	24.45825667014246	24.5262303774854	24.64598510272571
22	25.53160690248025	24.865136803162322	24.866916588010064	24.736339706347366
23	24.20177947194802	24.55722303193594	25.676763470462653	25.56423402565339
24	24.573345639263827	25.3044650123257	25.31674326238442	24.80544608602605
25	24.538121166826816	24.91476565002313	25.39408282619785	25.153030356952204
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	178.0
1	178.0
2	461.5
3	745.0
4	745.0
5	745.0
6	1848.5
7	2952.0
8	2952.0
9	2952.0
10	3447.5
11	3943.0
12	3943.0
13	3943.0
14	4921.0
15	5899.0
16	5899.0
17	5899.0
18	9437.0
19	12975.0
20	12975.0
21	12975.0
22	20349.5
23	27724.0
24	27724.0
25	27724.0
26	40422.0
27	53120.0
28	53120.0
29	53120.0
30	64932.0
31	76744.0
32	76744.0
33	76744.0
34	94157.5
35	111571.0
36	111571.0
37	111571.0
38	124507.5
39	137444.0
40	137444.0
41	137444.0
42	154790.5
43	172137.0
44	172137.0
45	172137.0
46	192667.5
47	213198.0
48	213198.0
49	213198.0
50	216238.5
51	219279.0
52	219279.0
53	219279.0
54	205363.5
55	191448.0
56	191448.0
57	191448.0
58	175669.0
59	159890.0
60	159890.0
61	159890.0
62	141425.5
63	122961.0
64	122961.0
65	122961.0
66	102271.5
67	81582.0
68	81582.0
69	81582.0
70	62350.5
71	43119.0
72	43119.0
73	43119.0
74	33892.5
75	24666.0
76	24666.0
77	24666.0
78	20015.5
79	15365.0
80	15365.0
81	15365.0
82	10844.5
83	6324.0
84	6324.0
85	6324.0
86	4687.0
87	3050.0
88	3050.0
89	3050.0
90	2020.0
91	990.0
92	990.0
93	990.0
94	602.0
95	214.0
96	214.0
97	214.0
98	455.5
99	697.0
100	697.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009358997521050341
2	0.003909454660691914
3	0.005804947829512236
4	0.016111691934972736
5	0.037850629214880804
6	0.048749714935597654
7	0.0709625255077108
8	0.09050979881117038
9	0.0991579863939131
10	0.12220007522738513
11	0.1221408410658595
12	0.1353500590860761
13	0.12427327088078237
14	0.12664263734180778
15	0.11515121000583456
16	0.11444040006752694
17	0.12634646653417958
18	0.13315839510962763
19	0.13452078082471722
20	0.1256948907573976
21	0.13440231250166595
22	0.15507503487411262
23	0.14417594915339577
24	0.1366532106396401
25	0.13475771747081977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1688215.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.02077484508212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.9052502329675	47.273216070092325
2	10.740853926104528	12.249036267286453
3	2.7477196191470403	4.7003130522239465
4	1.1540964631312507	2.6322989829485066
5	0.6014873287441149	1.7148636772244037
6	0.37316623531324894	1.276693673015022
7	0.2615575916062593	1.0439951578001716
8	0.17647969285497322	0.8050407064810141
9	0.13177881093606766	0.6762716916964343
>10	0.7263129453210094	7.68779659019621
>50	0.08215536041067978	3.2957290263323227
>100	0.0856938251435003	10.427373094860211
>500	0.01084177290908681	4.105008664509832
>1k	0.002606195410838176	2.1123633453332182
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2423	0.14352437337661375	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1988	0.11775751311296251	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1741	0.10312667521613064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.923416152563507E-5
2	0.0	0.0	0.0	0.0	5.923416152563507E-5
3	0.0	0.0	0.0	0.0	5.923416152563507E-5
4	0.0	0.0	0.0	0.0	5.923416152563507E-5
5	0.0	0.0	0.0	0.0	5.923416152563507E-5
6	0.0	0.0	0.0	0.0	1.1846832305127013E-4
7	0.0	0.0	0.0	0.0	1.1846832305127013E-4
8	0.0	0.0	0.0	0.0	1.1846832305127013E-4
9	0.0	0.0	0.0	5.923416152563507E-5	1.1846832305127013E-4
10	0.0	0.0	0.0	5.923416152563507E-5	1.1846832305127013E-4
11	0.0	0.0	0.0	5.923416152563507E-5	1.1846832305127013E-4
12	0.0	0.0	0.0	5.923416152563507E-5	2.3693664610254027E-4
13	0.0	0.0	0.0	5.923416152563507E-5	2.9617080762817533E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGTAT	310	0.0	12.872716	9
TACCGTG	95	1.0393469E-6	12.000563	7
GTATAGG	95	1.0479453E-6	11.992379	1
CGCATCG	295	0.0	11.916873	13
CCGTCGT	295	0.0	11.916873	9
ACACCGT	105	2.720626E-7	11.761758	6
ATACCGT	315	0.0	11.761758	6
TACCGTC	275	0.0	11.746004	7
CGGTCCA	355	0.0	11.508946	10
ACCGTCG	300	0.0	11.400872	8
TGCACCG	75	2.076541E-4	11.398505	5
TAAGGGT	100	1.936205E-6	11.396814	4
CGCAAGA	375	0.0	11.393098	2
GACGGTA	335	0.0	11.3441515	8
GCGTTAT	160	4.5474735E-11	11.274086	1
AAGACGG	390	0.0	11.203659	5
CAAGACG	390	0.0	11.201998	4
CGTCGTA	290	0.0	11.139774	10
AGAACCG	145	1.2205419E-9	11.136471	5
CCTATTC	325	0.0	11.101625	3
>>END_MODULE