FastQCFastQC Report
Thu 2 Feb 2017
SRR4062706_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062706_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688215
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18770.11118252118361702No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC18190.10774693981513019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23500.021.3502121
ACCGTCG4050.018.4698
ATACCGT4900.017.9590516
CGCCGGT5900.016.7795377
CCGTCGT4350.016.6895319
TAGGACG4250.016.5645834
CTACAGT7450.016.536794
CGCAATA6000.016.49987836
ATACGAA5900.016.40665840
CGTCGTA4600.016.2607510
GCCGGTC6550.016.1220198
TCTAGCG5750.016.06944728
CGGTCCA6100.015.86873510
GTCCTAC6800.015.857051
AACGCAG31650.015.7092046
TACCGTC5050.015.6830537
GGCGTTA4350.015.67804442
CTAGCGG5900.015.66090129
GTCGTAG4650.015.61278811
GATATAC1704.4788976E-815.5334371
Produced by FastQC (version 0.11.5)