##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062706_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1688215
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31210361239534	32.0	32.0	32.0	32.0	32.0
2	31.41401065622566	32.0	32.0	32.0	32.0	32.0
3	31.485835038783566	32.0	32.0	32.0	32.0	32.0
4	31.581588245572988	32.0	32.0	32.0	32.0	32.0
5	31.521148668860306	32.0	32.0	32.0	32.0	32.0
6	35.101229405022465	36.0	36.0	36.0	36.0	36.0
7	35.1137870472659	36.0	36.0	36.0	36.0	36.0
8	35.05880115980488	36.0	36.0	36.0	36.0	36.0
9	35.18554331053805	36.0	36.0	36.0	36.0	36.0
10	35.06057403825935	36.0	36.0	36.0	36.0	36.0
11	35.19411686307728	36.0	36.0	36.0	36.0	36.0
12	35.09983207115207	36.0	36.0	36.0	36.0	36.0
13	35.155721279576355	36.0	36.0	36.0	36.0	36.0
14	35.116611332087444	36.0	36.0	36.0	36.0	36.0
15	35.07037906901668	36.0	36.0	36.0	36.0	36.0
16	35.08359302576982	36.0	36.0	36.0	36.0	36.0
17	35.05804177785412	36.0	36.0	36.0	36.0	36.0
18	35.074906335982085	36.0	36.0	36.0	36.0	36.0
19	35.05655322337498	36.0	36.0	36.0	36.0	36.0
20	35.054724072467074	36.0	36.0	36.0	36.0	36.0
21	35.05191341150268	36.0	36.0	36.0	36.0	36.0
22	35.033752809920536	36.0	36.0	36.0	36.0	36.0
23	35.00015104711189	36.0	36.0	36.0	36.0	36.0
24	34.970171453280535	36.0	36.0	36.0	36.0	36.0
25	34.94959113620007	36.0	36.0	36.0	32.0	36.0
26	34.90323448139011	36.0	36.0	36.0	32.0	36.0
27	34.88981261273001	36.0	36.0	36.0	32.0	36.0
28	34.87030147226508	36.0	36.0	36.0	32.0	36.0
29	34.845445633405696	36.0	36.0	36.0	32.0	36.0
30	34.84087394081915	36.0	36.0	36.0	32.0	36.0
31	34.84097760060182	36.0	36.0	36.0	32.0	36.0
32	34.80770458738964	36.0	36.0	36.0	32.0	36.0
33	34.79488216844418	36.0	36.0	36.0	32.0	36.0
34	34.780963917510505	36.0	36.0	36.0	32.0	36.0
35	34.760670293771824	36.0	36.0	36.0	32.0	36.0
36	34.726189496006135	36.0	36.0	36.0	32.0	36.0
37	34.728563008858465	36.0	36.0	36.0	32.0	36.0
38	34.70501091389426	36.0	36.0	36.0	32.0	36.0
39	34.7093142757291	36.0	36.0	36.0	32.0	36.0
40	34.695006264012584	36.0	36.0	36.0	32.0	36.0
41	34.66622260790243	36.0	36.0	36.0	32.0	36.0
42	34.64681749658663	36.0	36.0	36.0	32.0	36.0
43	34.632872590280265	36.0	36.0	36.0	32.0	36.0
44	34.60527420974224	36.0	36.0	36.0	32.0	36.0
45	34.57832148156485	36.0	36.0	36.0	32.0	36.0
46	34.58177542552341	36.0	36.0	36.0	32.0	36.0
47	34.56553104906662	36.0	36.0	36.0	32.0	36.0
48	34.529956788679165	36.0	36.0	36.0	32.0	36.0
49	34.52065761766126	36.0	36.0	36.0	32.0	36.0
50	33.99124637560974	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	14.0
21	34.0
22	117.0
23	376.0
24	1058.0
25	2580.0
26	5142.0
27	9592.0
28	16314.0
29	25500.0
30	36990.0
31	53513.0
32	78954.0
33	131356.0
34	290368.0
35	1036305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.31754099205604	18.380709369773612	11.49756301878789	26.80418661938246
2	16.527667449185167	20.54516837772245	36.25459751833232	26.672566654760065
3	18.47360773556261	23.785054285533196	28.944682600374485	28.796655378529717
4	12.60682153259097	15.842432204940964	36.058838547031684	35.49190771543638
5	14.458229550146159	36.51501734080079	33.63351231922474	15.393240789828312
6	34.374577953849176	35.561815989062936	16.479465751769336	13.58414030531855
7	30.003364502367592	30.52338151442886	20.93318738027288	18.540066602930672
8	27.89504174233835	33.033786001063845	19.58614251510768	19.48502974149012
9	27.24664808688467	14.040628711390433	18.92709163228617	39.78563156943873
10	15.911362000693039	26.64583598653015	31.821657786478617	25.621144226298192
11	37.58324620975409	21.0726121969062	22.28797872308918	19.05616287025053
12	24.381965567181904	23.897370891740685	28.62644864546281	23.0942148956146
13	29.303317409216245	19.438223212090875	25.310875688226915	25.947583690465965
14	23.1291038167532	19.925424190639227	24.99865242282529	31.946819569782285
15	25.02536702967335	27.561181484585788	22.052345228540204	25.361106257200657
16	25.717814377908027	25.87448873514333	23.789742420248604	24.617954466700034
17	24.04332386573985	26.083170686198144	25.155622950868224	24.717882497193784
18	24.854772644479525	24.8967696650012	26.241740536602272	24.006717153917005
19	25.501195049208782	25.167173612365723	25.36317945285405	23.968451885571447
20	25.527850421895316	24.676122413318208	24.94083988117627	24.855187283610203
21	26.51889717838072	24.487165438051434	24.46643348151746	24.52750390205039
22	25.594370385288606	24.851218594787987	24.856786605971397	24.697624413952017
23	24.31982893174151	24.438771128084987	25.68357703254621	25.557822907627287
24	24.702600083520167	25.192644301821748	25.31537748450286	24.789378130155225
25	24.776346614619584	24.750935159325085	25.416194027419493	25.056524198635838
26	24.31811114105727	25.526073397049547	25.79363410466084	24.362181357232345
27	25.169424510503696	25.100712883133962	24.889957736425753	24.83990486993659
28	24.348439031758396	24.958669363795487	25.738013226988272	24.95487837745785
29	24.535026640564144	25.053858661367183	25.44190165352162	24.96921304454705
30	24.39126533054143	25.206919734749423	25.76875575682007	24.633059177889074
31	24.93082930787844	24.95784008553413	24.8477830134195	25.263547593167935
32	24.529458629380738	25.02436004892742	24.957129275595822	25.489052046096024
33	24.228015981376778	24.706154133211705	25.664918271665638	25.40091161374588
34	24.920344861288402	24.81940985004872	25.81560997858685	24.444635310076027
35	25.44788430383571	24.738199814597074	25.461982034278808	24.35193384728841
36	24.26385264909979	25.146145485024125	25.446403449797568	25.14359841607852
37	25.19122268194513	24.834810732045383	25.01440870979111	24.959557876218373
38	24.30620507459062	24.685185240031633	25.76449089719023	25.244118788187524
39	24.894400298540173	24.568375473503078	25.123399566998277	25.41382466095847
40	25.08187641976881	24.656101266722544	25.914116389203983	24.347905924304662
41	24.17387595774235	24.790266642578107	26.283085981347163	24.75277141833238
42	25.16462654342012	24.98822721039678	25.682036944346542	24.165109301836555
43	24.17974013973339	24.225646614915757	26.108285970685014	25.486327274665843
44	23.91804361411313	24.57637208530904	25.86323424445346	25.642350056124368
45	23.920472214735682	24.846065222735255	26.120488207959298	25.11297435456977
46	23.798077492637184	24.43940689913352	26.18646236373157	25.576053244497725
47	24.000260630310713	24.33522981373818	26.537378236776714	25.127131319174396
48	24.48923863370483	25.044558898007658	25.753532577307986	24.712669890979523
49	23.591485681622302	24.999600169409703	26.006284744537872	25.402629404430122
50	23.286635039535888	25.431684272067567	25.99405406782201	25.28762662057454
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	35.5
2	38.0
3	116.5
4	195.0
5	220.5
6	246.0
7	229.0
8	212.0
9	318.5
10	425.0
11	613.5
12	802.0
13	1283.5
14	1765.0
15	2295.5
16	2826.0
17	3319.5
18	3813.0
19	4290.0
20	4767.0
21	5833.5
22	6900.0
23	7726.0
24	8552.0
25	11020.0
26	13488.0
27	16678.5
28	19869.0
29	22547.0
30	25225.0
31	27544.5
32	29864.0
33	34443.5
34	39023.0
35	45034.5
36	51046.0
37	60002.5
38	68959.0
39	73290.0
40	77621.0
41	84273.5
42	90926.0
43	91818.0
44	92710.0
45	100868.5
46	109027.0
47	114831.5
48	120636.0
49	123554.5
50	126473.0
51	121611.5
52	116750.0
53	114764.0
54	112778.0
55	113799.0
56	114820.0
57	111638.0
58	108456.0
59	99023.5
60	89591.0
61	79124.5
62	68658.0
63	60228.0
64	51798.0
65	44575.0
66	37352.0
67	32957.0
68	28562.0
69	26070.5
70	23579.0
71	18502.5
72	13426.0
73	11978.0
74	10530.0
75	7851.5
76	5173.0
77	4672.5
78	4172.0
79	3586.5
80	3001.0
81	2362.5
82	1724.0
83	1448.5
84	1173.0
85	979.0
86	785.0
87	554.5
88	324.0
89	194.0
90	64.0
91	48.0
92	32.0
93	20.5
94	9.0
95	9.0
96	9.0
97	7.5
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015756286965818926
2	0.004501796275948265
3	7.108099383076207E-4
4	1.1846832305127012E-4
5	0.0
6	6.515757767819857E-4
7	5.923416152563506E-5
8	5.923416152563506E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.7770248457690517E-4
47	0.0
48	0.0
49	0.0
50	1.1846832305127012E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1688215.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.66478031476761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.79842069430585	50.8361278214454
2	10.409954685976704	12.630352282229246
3	2.5580835636446326	4.655567322459584
4	1.0246632642433082	2.4864388728773186
5	0.5390615810807181	1.635102619619653
6	0.3502034040120317	1.2747007541924225
7	0.2324989989112195	0.9873150484646768
8	0.16638976851712192	0.807519900297299
9	0.12198544797000622	0.6660198362429047
>10	0.6503258701430814	7.277283210934003
>50	0.06873125092317657	2.957373496204254
>100	0.06996695818904675	9.0702592844874
>500	0.007653858004913964	3.066890581456144
>1k	0.002060654078246067	1.6490489690896373
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1877	0.11118252118361702	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1819	0.10774693981513019	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1846832305127013E-4
2	0.0	0.0	0.0	0.0	1.1846832305127013E-4
3	0.0	0.0	0.0	0.0	1.1846832305127013E-4
4	0.0	0.0	0.0	0.0	1.1846832305127013E-4
5	0.0	0.0	0.0	0.0	1.1846832305127013E-4
6	0.0	0.0	0.0	0.0	1.1846832305127013E-4
7	0.0	0.0	0.0	0.0	1.1846832305127013E-4
8	0.0	0.0	0.0	0.0	1.1846832305127013E-4
9	0.0	0.0	0.0	0.0	1.1846832305127013E-4
10	0.0	0.0	0.0	0.0	1.1846832305127013E-4
11	0.0	0.0	0.0	0.0	1.1846832305127013E-4
12	0.0	0.0	0.0	0.0	3.554049691538104E-4
13	0.0	0.0	0.0	0.0	4.1463913067944547E-4
14	0.0	0.0	0.0	0.0	4.1463913067944547E-4
15	0.0	0.0	0.0	0.0	4.7387329220508053E-4
16	0.0	0.0	0.0	0.0	4.7387329220508053E-4
17	0.0	0.0	0.0	0.0	4.7387329220508053E-4
18	0.0	0.0	0.0	0.0	4.7387329220508053E-4
19	0.0	0.0	0.0	0.0	4.7387329220508053E-4
20	0.0	0.0	0.0	5.923416152563507E-5	5.923416152563507E-4
21	0.0	0.0	0.0	5.923416152563507E-5	5.923416152563507E-4
22	0.0	0.0	0.0	5.331074537307155E-4	5.923416152563507E-4
23	0.0	0.0	0.0	8.88512422884526E-4	5.923416152563507E-4
24	0.0	0.0	0.0	0.0015400881996665116	6.515757767819857E-4
25	0.0	0.0	0.0	0.0017177906842434168	6.515757767819857E-4
26	0.0	0.0	0.0	0.0030801763993330233	7.108099383076208E-4
27	0.0	0.0	0.0	0.005804947829512236	7.700440998332558E-4
28	0.0	0.0	0.0	0.017000204357857263	7.700440998332558E-4
29	0.0	0.0	0.0	0.03909454660691914	7.700440998332558E-4
30	0.0	0.0	0.0	0.08381633855877361	7.700440998332558E-4
31	0.0	0.0	0.0	0.17865023116131534	7.700440998332558E-4
32	0.0	0.0	0.0	0.28503478526135595	7.700440998332558E-4
33	0.0	0.0	0.0	0.39704658470633186	7.700440998332558E-4
34	0.0	0.0	0.0	0.5257031835400112	7.700440998332558E-4
35	0.0	0.0	0.0	0.6630672041179589	7.700440998332558E-4
36	0.0	0.0	0.0	0.856999848952888	7.700440998332558E-4
37	0.0	0.0	0.0	1.1410276534683081	7.700440998332558E-4
38	0.0	0.0	0.0	1.5301960946917306	7.700440998332558E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2350	0.0	21.350212	1
ACCGTCG	405	0.0	18.469	8
ATACCGT	490	0.0	17.959051	6
CGCCGGT	590	0.0	16.779537	7
CCGTCGT	435	0.0	16.689531	9
TAGGACG	425	0.0	16.564583	4
CTACAGT	745	0.0	16.53679	4
CGCAATA	600	0.0	16.499878	36
ATACGAA	590	0.0	16.406658	40
CGTCGTA	460	0.0	16.26075	10
GCCGGTC	655	0.0	16.122019	8
TCTAGCG	575	0.0	16.069447	28
CGGTCCA	610	0.0	15.868735	10
GTCCTAC	680	0.0	15.85705	1
AACGCAG	3165	0.0	15.709204	6
TACCGTC	505	0.0	15.683053	7
GGCGTTA	435	0.0	15.678044	42
CTAGCGG	590	0.0	15.660901	29
GTCGTAG	465	0.0	15.612788	11
GATATAC	170	4.4788976E-8	15.533437	1
>>END_MODULE