FastQCFastQC Report
Thu 2 Feb 2017
SRR4062705_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062705_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603223
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG39110.24394610107265177No Hit
GTATCAACGCAGAGTACTTTTTTTT37270.2324692198153345No Hit
TCCATGTACTCTGCGTTGATACCAC31870.1987870682992946No Hit
TATCAACGCAGAGTACTTTTTTTTT24750.15437652778184943No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21000.13098614478459952No Hit
GTCTTGCGCCGGTCCAAGAATTTCA18330.11433219209055759No Hit
GCGCAAGACGGACCAGAGCGAAAGC17270.10772051049666828No Hit
GAGTACATGGAAGCAGTGGTATCAA17120.10678489517677828No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17060.10641064904882228No Hit
GAATAGGACCGCGGTTCTATTTTGT16870.10522553631029494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA3450.014.8704210
TCCAACG2100.014.02680718
CGAACGA1205.0931703E-1113.4612116
CGTCGTA2150.013.25657610
CGGTTCT2800.013.23290412
GCCGGTC3950.013.2290238
AACGAAC1301.4551915E-1113.15501414
ACGAACG1301.4551915E-1113.15501415
CGCATCG2400.013.063251513
CCGTCGT2200.012.9556979
CCGGTCC4150.012.8204159
GTCCTAC10050.012.7539621
CGCCGGT3800.012.7511327
CGGAATT751.4773004E-512.66779115
ACCGTCG2250.012.6677918
TCGCGTA1352.7284841E-1112.6677919
GCGCGCT1051.9910658E-812.6677915
AGGCCCG4150.012.59108510
AGAATTT4400.012.52579517
TCGCCAG2600.012.42612117
Produced by FastQC (version 0.11.5)