##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062705_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1603223
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95103675533597	32.0	32.0	32.0	32.0	32.0
2	30.468760116340647	32.0	32.0	32.0	32.0	32.0
3	30.478654560220257	32.0	32.0	32.0	32.0	32.0
4	30.55574240140018	32.0	32.0	32.0	32.0	32.0
5	30.28693138758613	32.0	32.0	32.0	27.0	32.0
6	33.92239382793286	36.0	36.0	36.0	32.0	36.0
7	33.7693464976488	36.0	36.0	36.0	32.0	36.0
8	33.72863226138846	36.0	36.0	36.0	27.0	36.0
9	33.95118520630006	36.0	36.0	36.0	32.0	36.0
10	33.50795616080857	36.0	36.0	36.0	27.0	36.0
11	33.96326524756693	36.0	36.0	36.0	32.0	36.0
12	33.66000799639227	36.0	36.0	36.0	27.0	36.0
13	33.829457286977544	36.0	36.0	36.0	32.0	36.0
14	33.69545471840162	36.0	36.0	36.0	27.0	36.0
15	33.63185720264742	36.0	36.0	36.0	27.0	36.0
16	33.6374821219506	36.0	36.0	36.0	27.0	36.0
17	33.53511894477562	36.0	36.0	36.0	27.0	36.0
18	33.56352609711812	36.0	36.0	36.0	27.0	36.0
19	33.54317958262824	36.0	36.0	36.0	27.0	36.0
20	33.516936820392424	36.0	36.0	36.0	27.0	36.0
21	33.489051117654874	36.0	36.0	36.0	27.0	36.0
22	33.43926078904806	36.0	36.0	36.0	27.0	36.0
23	33.392135092872294	36.0	36.0	36.0	27.0	36.0
24	33.360117712882115	36.0	36.0	36.0	21.0	36.0
25	32.86395903751381	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	7.0
4	74.0
5	175.0
6	264.0
7	101.0
8	240.0
9	285.0
10	179.0
11	57.0
12	107.0
13	73.0
14	415.0
15	428.0
16	681.0
17	1007.0
18	1531.0
19	2356.0
20	3743.0
21	5673.0
22	8347.0
23	11806.0
24	15893.0
25	20832.0
26	27253.0
27	33962.0
28	43227.0
29	54649.0
30	67819.0
31	89371.0
32	123310.0
33	168422.0
34	344134.0
35	576800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.56256978709867	17.520725598687534	11.786113069135233	25.130591545078566
2	15.703212912530034	19.887896124893647	39.5507985498604	24.85809241271592
3	19.708408472659475	24.299980101463824	27.97222688323427	28.019384542642438
4	12.132609589378657	15.63824696920262	36.40955706345944	35.81958637795929
5	13.98006796260684	37.40701315199988	33.738579823616696	14.874339061776586
6	33.673728557066646	35.755851504975816	17.167950845298858	13.402469092658684
7	29.842376714222674	30.588142769133974	20.592393410943437	18.977087105699916
8	27.232776650538476	34.680006243171526	19.41788668643671	18.669330419853285
9	27.074993194696606	14.93145998107002	18.77470824602982	39.21883857820355
10	15.904796162171007	27.256791575960364	31.715482582516195	25.122929679352435
11	37.17279710235434	21.368513083119964	22.45063385998876	19.00805595453694
12	24.61925985514886	23.883324630939384	29.41548643051118	22.081929083400578
13	29.595078415735482	20.10500395622733	25.413780161159277	24.886137466877916
14	22.867686458386675	20.71423307657431	25.398159678549526	31.019920786489486
15	24.5838846672857	28.476182685480815	22.907059301781732	24.03287334545175
16	24.72802229024527	26.32941555546397	25.303699596232835	23.638862558057923
17	22.998700387885194	26.7728838836232	26.123452534181173	24.104963194310432
18	23.96401550401153	25.437845926699453	27.834147547925408	22.76399102136361
19	24.911576802799562	25.753744128281248	25.566561135514366	23.768117933404824
20	25.0632324819483	25.171149251140058	26.085256745734853	23.680361521176792
21	25.998546025052338	24.96733552845244	24.642002388316236	24.392116058178985
22	24.77681457822	25.80542591543348	25.467407213522748	23.95035229282377
23	23.410784633998414	25.445372566386602	26.022150418217038	25.12169238139795
24	23.971041702970716	26.152282767489808	26.02667855878071	23.849996970758767
25	24.353575880823126	25.643863405274818	25.792634637340456	24.209926076561597
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	478.0
1	478.0
2	828.0
3	1178.0
4	1178.0
5	1178.0
6	2466.0
7	3754.0
8	3754.0
9	3754.0
10	4055.5
11	4357.0
12	4357.0
13	4357.0
14	4897.5
15	5438.0
16	5438.0
17	5438.0
18	8741.5
19	12045.0
20	12045.0
21	12045.0
22	19867.0
23	27689.0
24	27689.0
25	27689.0
26	41390.5
27	55092.0
28	55092.0
29	55092.0
30	71298.0
31	87504.0
32	87504.0
33	87504.0
34	101000.0
35	114496.0
36	114496.0
37	114496.0
38	126502.5
39	138509.0
40	138509.0
41	138509.0
42	155662.5
43	172816.0
44	172816.0
45	172816.0
46	196252.5
47	219689.0
48	219689.0
49	219689.0
50	213551.5
51	207414.0
52	207414.0
53	207414.0
54	187080.0
55	166746.0
56	166746.0
57	166746.0
58	152584.5
59	138423.0
60	138423.0
61	138423.0
62	121264.0
63	104105.0
64	104105.0
65	104105.0
66	85980.5
67	67856.0
68	67856.0
69	67856.0
70	51234.5
71	34613.0
72	34613.0
73	34613.0
74	26977.0
75	19341.0
76	19341.0
77	19341.0
78	16062.0
79	12783.0
80	12783.0
81	12783.0
82	8779.0
83	4775.0
84	4775.0
85	4775.0
86	3544.5
87	2314.0
88	2314.0
89	2314.0
90	1568.0
91	822.0
92	822.0
93	822.0
94	526.5
95	231.0
96	231.0
97	231.0
98	493.0
99	755.0
100	755.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008295789169691302
2	0.004179081762175319
3	0.00561369191933998
4	0.016342080920745274
5	0.0359900026384352
6	0.04815300179700516
7	0.06936028238117842
8	0.09187742441319767
9	0.09549513698343899
10	0.11857364820739225
11	0.11994588400989757
12	0.13291974977903884
13	0.12162999158569957
14	0.12356359658013888
15	0.11252333580543691
16	0.11258571016009625
17	0.12325172480684221
18	0.13030002688334685
19	0.13198413445914886
20	0.12406259141741355
21	0.13067427301130285
22	0.15119543569422345
23	0.14096604153009282
24	0.13485335477347815
25	0.13142276526721486
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1603223.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.7879450744807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.95486800584167	38.575916644201186
2	14.181245573613172	14.404726425607766
3	4.6780054666939686	7.127588541017213
4	1.931154476473588	3.923174699259125
5	0.9799190613241986	2.4884037731985043
6	0.5571527416186588	1.6977985703654868
7	0.33721307702828685	1.1988451464156484
8	0.22865263711893455	0.9290238060105294
9	0.15727925775561374	0.7189101273822484
>10	0.7982436786034423	7.40478112382431
>50	0.08718691684010883	3.0937747404436045
>100	0.09207032521485649	9.987250908867372
>500	0.0112159358731443	3.941932195962001
>1k	0.005792846000415188	4.507873297445056
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3911	0.24394610107265177	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3727	0.2324692198153345	No Hit
TCCATGTACTCTGCGTTGATACCAC	3187	0.1987870682992946	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2475	0.15437652778184943	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2100	0.13098614478459952	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1833	0.11433219209055759	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1727	0.10772051049666828	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1712	0.10678489517677828	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1706	0.10641064904882228	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1687	0.10522553631029494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.237435465933311E-5	0.0
8	0.0	0.0	0.0	1.2474870931866621E-4	0.0
9	0.0	0.0	0.0	1.2474870931866621E-4	0.0
10	6.237435465933311E-5	0.0	0.0	2.4949741863733243E-4	0.0
11	6.237435465933311E-5	0.0	0.0	2.4949741863733243E-4	0.0
12	6.237435465933311E-5	0.0	0.0	2.4949741863733243E-4	3.1187177329666555E-4
13	6.237435465933311E-5	0.0	0.0	3.1187177329666555E-4	3.1187177329666555E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCCA	345	0.0	14.87042	10
TCCAACG	210	0.0	14.026807	18
CGAACGA	120	5.0931703E-11	13.46121	16
CGTCGTA	215	0.0	13.256576	10
CGGTTCT	280	0.0	13.232904	12
GCCGGTC	395	0.0	13.229023	8
AACGAAC	130	1.4551915E-11	13.155014	14
ACGAACG	130	1.4551915E-11	13.155014	15
CGCATCG	240	0.0	13.0632515	13
CCGTCGT	220	0.0	12.955697	9
CCGGTCC	415	0.0	12.820415	9
GTCCTAC	1005	0.0	12.753962	1
CGCCGGT	380	0.0	12.751132	7
CGGAATT	75	1.4773004E-5	12.667791	15
ACCGTCG	225	0.0	12.667791	8
TCGCGTA	135	2.7284841E-11	12.667791	9
GCGCGCT	105	1.9910658E-8	12.66779	15
AGGCCCG	415	0.0	12.591085	10
AGAATTT	440	0.0	12.525795	17
TCGCCAG	260	0.0	12.426121	17
>>END_MODULE