FastQCFastQC Report
Thu 2 Feb 2017
SRR4062705_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062705_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603223
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA18790.1172014124048869No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC18770.11707666369556824No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17320.10803238226996494No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16980.1059116542115476No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16320.10179494680403163No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16170.10085933148414163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC11850.020.7963891
TTAGGAC6350.020.7872983
GGCGCGA550.00448436919.999939
GACGGTA4000.019.7999028
TAGGACG10050.019.482494
CGCAATA4650.019.39775536
CGACGGT4250.019.1528457
ACGACGG4250.019.1528456
TCCTACA13100.019.1461372
TAGCGGC4850.019.05145330
TGTAGGA11700.018.9925442
GACCGTT708.125176E-418.8570487
CGTCGTA3650.018.6848410
TAGGACC12250.018.677464
CTAGCGG4950.018.66657629
GTAGGAC12050.018.6223143
TACGACG4400.018.4999075
ATACGAA4900.018.40807240
TAGTACG600.00741334318.3332424
GTCCTAA6250.018.3067631
Produced by FastQC (version 0.11.5)